LOCUS       sequence-123993-         977 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses tdTomato in pTREX-b vector which confers resistance to 
            blasticidin. This vector is used for cloning a specific sgRNA by 
            BamHI, to transfect Cas9-expressing Trypanosoma cruzi 
            epimastigotes..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 977)
  AUTHORS   Lander N, Li ZH, Niyogi S, Docampo R
  TITLE     CRISPR/Cas9-Induced Disruption of Paraflagellar Rod Protein 1 and 2 
            Genes in Trypanosoma cruzi Reveals Their Role in Flagellar 
            Attachment.
  JOURNAL   MBio. 2015 Jul 21;6(4). pii: e01012-15. doi: 10.1128/mBio.01012-15.
  PUBMED    26199333
REFERENCE   2  (bases 1 to 977)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "MBio."; 
            date: "2015-07-21"; volume: "6(4). pii: e01012-15. doi"; pages: " 
            10.1128/mBio.01012-15"
COMMENT     Sequence Label: tdTomato/pTREX-b
FEATURES             Location/Qualifiers
     source          1..977
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     702..718
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     703..719
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(814..837)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 aaggcttggc catgaattga tctcgcaatc ccaaattctg ttcaatgtaa ttgttgttgg
       61 tagatggctt tcaaagtaaa aatatcactt tctgcattcg tgcaggaaac gtgggaaaag
      121 agagacaagt ggaataatgg ggaaagaaaa gcagaaaaac taaaaagatg tggcaaaaag
      181 ggaagtgagc aaaaattacc acaagctgca tcacacaccc aattaaatca tctacatttt
      241 ccttgcaata ccccaaatcc tcgtacattg attaaacatt atttcactca tccttccgca
      301 tcattaaatt gctgcttttt gtttcccaaa gatgaccata aatataattt caaaaaaaaa
      361 aaaaaagaaa aaaaagttat atattttttt gttgttgtcc ttaaagggaa aagtaaaatt
      421 gttttgctct tgacgctgtg tcaattggat cgtggcagca ccggtaacga tcatcacggg
      481 acacgcatcg tgtattataa gatccctatt cgcaaaagaa agacctgaag tcatcgccac
      541 gcctacaacc ttgccgaacg atccttcgag gccatgtggc gtacaaggtc caccacgcgg
      601 tgggagtatc cccactcgtt gtcgtaccac gacacaatct tgaagaagcg gcgctccttc
      661 ggcaggttgt tctgcagcgt tgccttggag tcgtaaatgg ctcgaggtcg acggtatcga
      721 taagctttta cttgtacaac tcgtccatgc cgtacaggaa caggtggtgg cggccctcgg
      781 agcgctcgta ctgttccacg atggtgtagt cctcgttgtg ggaggtgatg tccagcttgg
      841 tgtccacgta gtagtagccg ggcagttgca cgggcttctt ggccatgtag atggtcttga
      901 actccaccag gtagtggccg ccgtccttca gcttcagggc ctggtggatc tcgcccttca
      961 gcacgccgtc gcggggg
//
