LOCUS       sequence-124002-         933 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  regulated expression of genes in Escherichia coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Stark MJ
  TITLE     Multicopy expression vectors carrying the lac repressor gene for 
            regulated high-level expression of genes in Escherichia coli.
  JOURNAL   Gene. 1987;51(2-3):255-67.
  PUBMED    3110013
REFERENCE   2  (bases 1 to 933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene."; 
            date: "1987"; volume: "51(2-3)"; pages: "255-67"
COMMENT     Sequence Label: pttq18
FEATURES             Location/Qualifiers
     source          1..933
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(49..71)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    60..76
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(84..90,91..106,107..112))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     primer_bind     complement(383..400)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(634..653)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcgactctag aggatccccg ggtaccgagc tcgaattcat cgctgtttcc tgtgtgaaat
       61 tgttatccgc tcacaattcc acacattata cgagccgatg attaattgtc aacagctcat
      121 ttcagaatat ttgccagaac cgttatgatg tcggcgcaaa aaacattatc cagaacggga
      181 gtgcgccttg agcgacacga attatgcagt gatttacgac ctgcacagcc ataccacagc
      241 ttccgatggc tgcctgacgc cagaagcatt ggtgcaccgt gcagtcgatg ataagctgtc
      301 aaacatgaga attaattctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
      361 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
      421 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
      481 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
      541 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
      601 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
      661 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
      721 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
      781 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
      841 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
      901 cagcagccac tggtaacagg attagcagag cga
//
