LOCUS       sequence-124012-        1021 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Golden Gate entry vector; 1st gRNA under OsU6 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Lowder LG, Paul JW, Baltes NJ, Voytas DF, Zhang Y, Zhang D, Tang X, 
            Zheng X, Hsieh TF, Qi Y
  TITLE     A CRISPR/Cas9 toolbox for multiplexed plant genome editing and 
            transcriptional regulation.
  JOURNAL   Plant Physiol. 2015 Aug 21. pii: pp.00636.2015.
  PUBMED    26297141
REFERENCE   2  (bases 1 to 1021)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2015 Aug 21. pii: pp.00636.2015."
COMMENT     Sequence Label: pYPQ132C
FEATURES             Location/Qualifiers
     source          1..1021
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        complement(190..265)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     686..702
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     686..702
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     707..726
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        707..725
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgacgcgta
       61 actcacgtta agggattttg gtcatgggtg gctcgagggt ctcggtccaa gcttatcgat
      121 accctcgacc tcgagcggcc gccagtgtga tggatatctg cagaattgcc cttcgaaggg
      181 acaaaaaaag caccgactcg gtgccacttt ttcaagttga taacggacta gccttatttt
      241 aacttgctat ttctagctct aaaacagaga cggtcgactc agactagatc tcgtctccac
      301 acaagcgaca gcgcgcgggt ttataagttg gtcgcgttcg agttagctgg gcaatgtggt
      361 actaaactgt tcctcccgcc tctcgcgctc acactcgccc tgtgggccgc tcaccgtgca
      421 cgtacttggg cctcccgctc ccccgcatgc atccagccca tcacagcgaa gagaatcggg
      481 cttttcttct ccccatctcc ctacacaacc accaaataca gccaggccgt tggttcatga
      541 tccaagggca attccagcac actggcggcc gttactagtg gatccgagct cggtacccat
      601 gggagacctc tagaggaggc atgatgatat atttttatct tgtgcaatgt aacatcagag
      661 attttgagac acgggccaga gctgccagga aacagctatg accatgtaat acgactcact
      721 ataggggata ttttattttc tcccaatcag gcttgatccc cagtaagtca aaaaatagct
      781 cgacatactg ttcttccccg atcaagggtt ggtttgcgca ttcacagttc tccgcaagaa
      841 ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt
      901 caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat
      961 agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc
     1021 g
//
