LOCUS       sequence-124014-        1107 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Golden Gate entry vector; 1st gRNA under OsU6 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Lowder LG, Paul JW, Baltes NJ, Voytas DF, Zhang Y, Zhang D, Tang X, 
            Zheng X, Hsieh TF, Qi Y
  TITLE     A CRISPR/Cas9 toolbox for multiplexed plant genome editing and 
            transcriptional regulation.
  JOURNAL   Plant Physiol. 2015 Aug 21. pii: pp.00636.2015.
  PUBMED    26297141
REFERENCE   2  (bases 1 to 1107)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2015 Aug 21. pii: pp.00636.2015."
COMMENT     Sequence Label: pYPQ133C
FEATURES             Location/Qualifiers
     source          1..1107
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        complement(188..263)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     684..700
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     684..700
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     705..724
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        705..723
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gacgcgtaac
       61 tcacgttaag ggattttggt catgggtggc tcgagggtct ccctggaagc ttatcgatac
      121 cctcgacctc gagcggccgc cagtgtgatg gatatctgca gaattgccct tcgaagggac
      181 aaaaaaagca ccgactcggt gccacttttt caagttgata acggactagc cttattttaa
      241 cttgctattt ctagctctaa aacagagacg gtcgactcag actagatctc gtctccacac
      301 aagcgacagc gcgcgggttt ataagttggt cgcgttcgag ttagctgggc aatgtggtac
      361 taaactgttc ctcccgcctc tcgcgctcac actcgccctg tgggccgctc accgtgcacg
      421 tacttgggcc tcccgctccc ccgcatgcat ccagcccatc acagcgaaga gaatcgggct
      481 tttcttctcc ccatctccct acacaaccac caaatacagc caggccgttg gttcatgatc
      541 caagggcaat tccagcacac tggcggccgt tactagtgga tccgagctcg gtaccgtcct
      601 gagacctcta gaggaggcat gatgatatat ttttatcttg tgcaatgtaa catcagagat
      661 tttgagacac gggccagagc tgccaggaaa cagctatgac catgtaatac gactcactat
      721 aggggatatt ttattttctc ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg
      781 acatactgtt cttccccgat caagggttgg tttgcgcatt cacagttctc cgcaagaatt
      841 gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca
      901 ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag
      961 ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc
     1021 cgtgacgatc agcggtccaa tgatcgaagt taggctggta agagccgcga gcgatccttg
     1081 aagctgtccc tgatggtcgt catctac
//
