LOCUS       sequence-124016-        1059 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Golden Gate entry vector; 1st gRNA under OsU3 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Lowder LG, Paul JW, Baltes NJ, Voytas DF, Zhang Y, Zhang D, Tang X, 
            Zheng X, Hsieh TF, Qi Y
  TITLE     A CRISPR/Cas9 toolbox for multiplexed plant genome editing and 
            transcriptional regulation.
  JOURNAL   Plant Physiol. 2015 Aug 21. pii: pp.00636.2015.
  PUBMED    26297141
REFERENCE   2  (bases 1 to 1059)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2015 Aug 21. pii: pp.00636.2015."
COMMENT     Sequence Label: pYPQ132D
FEATURES             Location/Qualifiers
     source          1..1059
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        complement(187..262)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(295..672)
                     /label=OsU3 promoter
                     /note="Oryza sativa (rice) snRNA U3 promoter"
     primer_bind     761..777
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     761..777
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     782..801
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        782..800
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg acgcgtaact
       61 cacgttaagg gattttggtc atgggtggct cgagggtctc ggtccaagct tatcgatacc
      121 ctcgacctcg agcggccgcc agtgtgatgg atatctgcag aattgccctt cgaagggaca
      181 aaaaaagcac cgactcggtg ccactttttc aagttgataa cggactagcc ttattttaac
      241 ttgctatttc tagctctaaa acagagacgg tcgactcaga ctagatctcg tctctgccac
      301 ggatcatctg cacaactctt ttaaaccagc tttgatctat gtggatagcc gaggtggtac
      361 taatactagt ctttgttgtc gtccaattgc gtaatgggcc ggcccatact gcaatacatg
      421 tcctgaaagg cttcatggcc cactacgaaa tgcttttctc ctacagttta tcttactcca
      481 catcacgtgg tttccaacgt acccagtgtt cccggcttcc agcatttgct ggtagcacca
      541 gtagaagacg cctgtcctgt gctatggtcc ctgactgcac atctgattcc tccaagatcc
      601 atgcatgcct gataacttta agttgcttca gaagaacttt aagtgatctg ttcgtatgtt
      661 taaagatccc ttcatgggag acctctagag gaggcatgat gatatatttt tatcttgtgc
      721 aatgtaacat cagagatttt gagacacggg ccagagctgc caggaaacag ctatgaccat
      781 gtaatacgac tcactatagg ggatatttta ttttctccca atcaggcttg atccccagta
      841 agtcaaaaaa tagctcgaca tactgttctt ccccgatcaa gggttggttt gcgcattcac
      901 agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg
      961 ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg
     1021 aggcagacaa ggtatagggc ggcgcctaca atccatgcc
//
