LOCUS       sequence-124018-         996 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Golden Gate entry vector; 1st gRNA under OsU3 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Lowder LG, Paul JW, Baltes NJ, Voytas DF, Zhang Y, Zhang D, Tang X, 
            Zheng X, Hsieh TF, Qi Y
  TITLE     A CRISPR/Cas9 toolbox for multiplexed plant genome editing and 
            transcriptional regulation.
  JOURNAL   Plant Physiol. 2015 Aug 21. pii: pp.00636.2015.
  PUBMED    26297141
REFERENCE   2  (bases 1 to 996)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2015 Aug 21. pii: pp.00636.2015."
COMMENT     Sequence Label: pYPQ133D
FEATURES             Location/Qualifiers
     source          1..996
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(350..369)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(351..369)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(374..390)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(374..390)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        477..854
                     /label=OsU3 promoter
                     /note="Oryza sativa (rice) snRNA U3 promoter"
     misc_RNA        887..962
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 cgctcgcggc tcttaccagc ctaacttcga tcattggacc gctgatcgtc acggcgattt
       61 atgccgcctc ggcgagcaca tggaacgggt tggcatggat tgtaggcgcc gccctatacc
      121 ttgtctgcct ccccgcgttg cgtcgcggtg catggagccg ggccacctcg acctgaatgg
      181 aagccggcgg cacctcgcta acggattcac cactccaaga attggagcca atcaattctt
      241 gcggagaact gtgaatgcgc aaaccaaccc ttgatcgggg aagaacagta tgtcgagcta
      301 ttttttgact tactggggat caagcctgat tgggagaaaa taaaatatcc cctatagtga
      361 gtcgtattac atggtcatag ctgtttcctg gcagctctgg cccgtgtctc aaaatctctg
      421 atgttacatt gcacaagata aaaatatatc atcatgcctc ctctagaggt ctcaggacgg
      481 gatctttaaa catacgaaca gatcacttaa agttcttctg aagcaactta aagttatcag
      541 gcatgcatgg atcttggagg aatcagatgt gcagtcaggg accatagcac aggacaggcg
      601 tcttctactg gtgctaccag caaatgctgg aagccgggaa cactgggtac gttggaaacc
      661 acgtgatgtg gagtaagata aactgtagga gaaaagcatt tcgtagtggg ccatgaagcc
      721 tttcaggaca tgtattgcag tatgggccgg cccattacgc aattggacga caacaaagac
      781 tagtattagt accacctcgg ctatccacat agatcaaagc tggtttaaaa gagttgtgca
      841 gatgatccgt ggcagagacg agatctagtc tgagtcgacc gtctctgttt tagagctaga
      901 aatagcaagt taaaataagg ctagtccgtt atcaacttga aaaagtggca ccgagtcggt
      961 gctttttttg tcccttcgaa gggcaattct gcagat
//
