LOCUS       sequence-124021-         807 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  construction of CYC1 promoter in HCKan-P receiving vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 807)
  AUTHORS   Guo Y, Dong J, Zhou T, Auxillos J, Li T, Zhang W, Wang L, Shen Y, 
            Luo Y, Zheng Y, Lin J, Chen GQ, Wu Q, Cai Y, Dai J
  TITLE     YeastFab: the design and construction of standard biological parts 
            for metabolic engineering in Saccharomyces cerevisiae.
  JOURNAL   Nucleic Acids Res. 2015 May 8. pii: gkv464.
  PUBMED    25956650
REFERENCE   2  (bases 1 to 807)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 May 8. pii: gkv464."
COMMENT     Sequence Label: HCKan-P-CYC1
FEATURES             Location/Qualifiers
     source          1..807
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      414..445
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        446..548
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(622..641)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 aaggcccacc cgtgaaggtg agccagtgag ttgattgcag tccagttacg ctggagtccg
       61 tctcgggctg agctcatttg gcgagcgttg gttggtggat caagcccacg cgtaggcaat
      121 cctcgagcag atccgccagg cgtgtatata tagcgtggat ggccaggcaa ctttagtgct
      181 gacacataca ggcatatata tatgtgtgcg acgacacatg atcatatggc atgcatgtgc
      241 tctgtatgta tataaaactc ttgttttctt cttttctcta aatattcttt ccttatacat
      301 taggaccttt gcagcataaa ttactatact tctatagaca cgcaaacaca aatacacaca
      361 ctaagatgcg agacgactga ccatttaaat catacctgac ctccatagca gaaagtcaaa
      421 agcctccgac cggaggcttt tgacttgatc ggcacgtaag aggttccaac tttcaccata
      481 atgaaataag atcactaccg ggcgtatttt ttgagttatc gagattttca ggagctaagg
      541 aagctaaaat gagccatatt caacgggaaa cgtcttgctc gaggccgcga ttaaattcca
      601 acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg caatcaggtg
      661 cgacaatcta tcgattgtat gggaagcccg atgcgccaga gttgtttctg aaacatggca
      721 aaggtagcgt tgccaatgat gttacagatg agatggtcag gctaaactgg ctgacggaat
      781 ttatgcctct tccgaccatc aagcatt
//
