LOCUS       sequence-124022-         915 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry vector with pcoCas9D10A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Lowder LG, Paul JW, Baltes NJ, Voytas DF, Zhang Y, Zhang D, Tang X, 
            Zheng X, Hsieh TF, Qi Y
  TITLE     A CRISPR/Cas9 toolbox for multiplexed plant genome editing and 
            transcriptional regulation.
  JOURNAL   Plant Physiol. 2015 Aug 21. pii: pp.00636.2015.
  PUBMED    26297141
REFERENCE   2  (bases 1 to 915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2015 Aug 21. pii: pp.00636.2015."
COMMENT     Sequence Label: pYPQ151
FEATURES             Location/Qualifiers
     source          1..915
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    22..46
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             68..115
                     /codon_start=1
                     /product="two tandem FLAG(R) epitope tags"
                     /label=2xFLAG
                     /translation="DYKDDDDKDYKDDDDK"
     CDS             122..142
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gagcaatgct tttttataat gccaactttg tacaaaaaag caggctccga attcgccctt
       61 caccatggat tacaaggatg atgatgataa ggattacaag gatgatgatg ataagatggc
      121 tccaaagaag aagagaaagg ttggaatcca cggagttcca gctgctgata agaagtactc
      181 tatcggactt gccatcggaa ccaactctgt tggatgggct gttatcaccg atgagtacaa
      241 ggttccatct aagaagttca aggttcttgg aaacaccgat agacactcta tcaagaagaa
      301 ccttatcggt gctcttcttt tcgattctgg agagaccgct gaggctacca gattgaagag
      361 aaccgctaga agaagataca ccagaagaaa gaacagaatc tgctaccttc aggaaatctt
      421 ctctaacgag atggctaagg ttgatgattc tttcttccac agacttgagg agtctttcct
      481 tgttgaggag gataagaagc acgagagaca cccaatcttc ggaaacatcg ttgatgaggt
      541 tgcttaccac gagaagtacc caaccatcta ccaccttaga aagaagttgg ttgattctac
      601 cgataaggct gatcttagac ttatctacct tgctcttgct cacatgatca agttcagagg
      661 acacttcctt atcgagggag accttaaccc agataactct gatgttgata agttgttcat
      721 ccagcttgtt cagacctaca accagctttt cgaggagaac ccaatcaacg cttctggagt
      781 tgatgctaag gctatccttt ctgctagact ttctaagtct cgtagacttg agaaccttat
      841 cgctcagctt ccaggagaga agaagaacgg acttttcgga aaccttatcg ctctttctct
      901 tggacttacc ccaaa
//
