LOCUS       sequence-124023-        1083 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry vector with pcoCas9D10A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1083)
  AUTHORS   Lowder LG, Paul JW, Baltes NJ, Voytas DF, Zhang Y, Zhang D, Tang X, 
            Zheng X, Hsieh TF, Qi Y
  TITLE     A CRISPR/Cas9 toolbox for multiplexed plant genome editing and 
            transcriptional regulation.
  JOURNAL   Plant Physiol. 2015 Aug 21. pii: pp.00636.2015.
  PUBMED    26297141
REFERENCE   2  (bases 1 to 1083)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2015 Aug 21. pii: pp.00636.2015."
COMMENT     Sequence Label: pYPQ151
FEATURES             Location/Qualifiers
     source          1..1083
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(13..136)
                     /gene="mutant version of attR"
                     /label=attR5
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      complement(181..433)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(444..491)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 tcagctggat ggcatagtga ctggatatgt tgtgttttac agtattatgt agtctgtttt
       61 ttatgcaaaa tctaatttaa tatattgata tttatatcat tttacgtttc tcgttcaact
      121 tttgtataca aagttgcgaa ttcgcccttt ccggaagctg tcgagggggg atcaattccc
      181 gatctagtaa catagatgac accgcgcgcg ataatttatc ctagtttgcg cgctatattt
      241 tgttttctat cgcgtattaa atgtataatt gcgggactct aatcataaaa acccatctca
      301 taaataacgt catgcattac atgttaatta ttacatgctt aacgtaattc aacagaaatt
      361 atatgataat catcgcaaga ccggcaacag gattcaatct taagaaactt tattgccaaa
      421 tgtttgaacg atcggacgtc tcacttcttc ttcttagcct gtccagcctt cttggtagca
      481 gctggtctct tatctcctcc aagctgagaa agatcgattc tggtctcgta aagtccggtg
      541 atagactggt ggataagggt agcatcaaga acctccttgg tagaggtgta tctttttcta
      601 tcgatggtgg tatcgaagta cttgaaagca gctggagcac caaggttggt aagggtgaaa
      661 aggtggatga tgttctcagc ctgctctctg attggcttat ctctgtgctt gttgtaagca
      721 gaaagaacct tatcaaggtt agcatcagca aggataactc tcttagagaa ctcagagatt
      781 tgctcgatga tctcatcaag gtagtgcttg tgctgctcaa cgaaaagctg cttctgctcg
      841 ttatcctctg gagatccctt caacttctcg tagtgagaag caaggtaaag gaagttaacg
      901 tacttagatg gaagagcaag ctcgtttccc ttctgaagct ctccagcaga agcaagcatt
      961 ctctttcttc cgttctcaag ctcgaaaaga gagtactttg gcaacttgat gataagatcc
     1021 ttcttaacct ccttgtatcc cttagcctca aggaaatcga ttgggttctt ctcgaaagaa
     1081 gaa
//
