LOCUS       sequence-124025-        1078 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry vector with pco-dCas9-3X(SRDX).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Lowder LG, Paul JW, Baltes NJ, Voytas DF, Zhang Y, Zhang D, Tang X, 
            Zheng X, Hsieh TF, Qi Y
  TITLE     A CRISPR/Cas9 toolbox for multiplexed plant genome editing and 
            transcriptional regulation.
  JOURNAL   Plant Physiol. 2015 Aug 21. pii: pp.00636.2015.
  PUBMED    26297141
REFERENCE   2  (bases 1 to 1078)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2015 Aug 21. pii: pp.00636.2015."
COMMENT     Sequence Label: pYPQ153
FEATURES             Location/Qualifiers
     source          1..1078
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(13..136)
                     /gene="mutant version of attR"
                     /label=attR5
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      complement(181..433)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(594..641)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 tcagctggat ggcatagtga ctggatatgt tgtgttttac agtattatgt agtctgtttt
       61 ttatgcaaaa tctaatttaa tatattgata tttatatcat tttacgtttc tcgttcaact
      121 tttgtataca aagttgcgaa ttcgcccttt ccggaagctg tcgagggggg atcaattccc
      181 gatctagtaa catagatgac accgcgcgcg ataatttatc ctagtttgcg cgctatattt
      241 tgttttctat cgcgtattaa atgtataatt gcgggactct aatcataaaa acccatctca
      301 taaataacgt catgcattac atgttaatta ttacatgctt aacgtaattc aacagaaatt
      361 atatgataat catcgcaaga ccggcaacag gattcaatct taagaaactt tattgccaaa
      421 tgtttgaacg atcggacgtc ctaagcgaat ccgagtctaa gctcaagatc gaggtcaaga
      481 gcaaatccga gtctaagttc aaggtcgaga tcgagagcaa atccaagtct gagttcgagg
      541 tcaagatcaa ggtcgactga gccagaaccc gaccccgatc cagagccgtc tcccttcttc
      601 ttcttagcct gtccagcctt cttggtagca gctggtctct tatctcctcc aagctgagaa
      661 agatcgattc tggtctcgta aagtccggtg atagactggt ggataagggt agcatcaaga
      721 acctccttgg tagaggtgta tctttttcta tcgatggtgg tatcgaagta cttgaaagca
      781 gctggagcac caaggttggt aagggtgaaa aggtggatga tgttctcagc ctgctctctg
      841 attggcttat ctctgtgctt gttgtaagca gaaagaacct tatcaaggtt agcatcagca
      901 aggataactc tcttagagaa ctcagagatt tgctcgatga tctcatcaag gtagtgcttg
      961 tgctgctcaa cgaaaagctg cttctgctcg ttatcctctg gagatccctt caacttctcg
     1021 tagtgagaag caaggtaaag gaagttaacg tacttagatg gaagagcaag ctcgtttc
//
