LOCUS       sequence-124030-        1071 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry vector with hSpCas9 (Human codon optimized).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Lowder LG, Paul JW, Baltes NJ, Voytas DF, Zhang Y, Zhang D, Tang X, 
            Zheng X, Hsieh TF, Qi Y
  TITLE     A CRISPR/Cas9 toolbox for multiplexed plant genome editing and 
            transcriptional regulation.
  JOURNAL   Plant Physiol. 2015 Aug 21. pii: pp.00636.2015.
  PUBMED    26297141
REFERENCE   2  (bases 1 to 1071)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2015 Aug 21. pii: pp.00636.2015."
COMMENT     Sequence Label: pYPQ158
FEATURES             Location/Qualifiers
     source          1..1071
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    70..94
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      122..131
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             131..196
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             203..223
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 atgattttat tttgactgat agtgacctgt tcgttgcaac aaattgatga gcaatgcttt
       61 tttataatgc caactttgta caaaaaagca ggctccgaat tcgcccttca ccatgaccgg
      121 tgccaccatg gactataagg accacgacgg agactacaag gatcatgata ttgattacaa
      181 agacgatgac gataagatgg ccccaaagaa gaagcggaag gtcggtatcc acggagtccc
      241 agcagccgac aagaagtaca gcatcggcct ggacatcggc accaactctg tgggctgggc
      301 cgtgatcacc gacgagtaca aggtgcccag caagaaattc aaggtgctgg gcaacaccga
      361 ccggcacagc atcaagaaga acctgatcgg agccctgctg ttcgacagcg gcgaaacagc
      421 cgaggccacc cggctgaaga gaaccgccag aagaagatac accagacgga agaaccggat
      481 ctgctatctg caagagatct tcagcaacga gatggccaag gtggacgaca gcttcttcca
      541 cagactggaa gagtccttcc tggtggaaga ggataagaag cacgagcggc accccatctt
      601 cggcaacatc gtggacgagg tggcctacca cgagaagtac cccaccatct accacctgag
      661 aaagaaactg gtggacagca ccgacaaggc cgacctgcgg ctgatctatc tggccctggc
      721 ccacatgatc aagttccggg gccacttcct gatcgagggc gacctgaacc ccgacaacag
      781 cgacgtggac aagctgttca tccagctggt gcagacctac aaccagctgt tcgaggaaaa
      841 ccccatcaac gccagcggcg tggacgccaa ggccatcctg tctgccagac tgagcaagag
      901 cagacggctg gaaaatctga tcgcccagct gcccggcgag aagaagaatg gcctgttcgg
      961 aaacctgatt gccctgagcc tgggcctgac ccccaacttc aagagcaact tcgacctggc
     1021 cgaggatgcc aaactgcagc tgagcaagga cacctacgac gacgacctgg a
//
