LOCUS       sequence-124031-        1038 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry vector with hSpCas9 (Human codon optimized).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Lowder LG, Paul JW, Baltes NJ, Voytas DF, Zhang Y, Zhang D, Tang X, 
            Zheng X, Hsieh TF, Qi Y
  TITLE     A CRISPR/Cas9 toolbox for multiplexed plant genome editing and 
            transcriptional regulation.
  JOURNAL   Plant Physiol. 2015 Aug 21. pii: pp.00636.2015.
  PUBMED    26297141
REFERENCE   2  (bases 1 to 1038)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2015 Aug 21. pii: pp.00636.2015."
COMMENT     Sequence Label: pYPQ158
FEATURES             Location/Qualifiers
     source          1..1038
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(13..136)
                     /gene="mutant version of attR"
                     /label=attR5
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      complement(181..433)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(452..499)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 tcagctggat ggcatagtga ctggatatgt tgtgttttac agtattatgt agtctgtttt
       61 ttatgcaaaa tctaatttaa tatattgata tttatatcat tttacgtttc tcgttcaact
      121 tttgtataca aagttgcgaa ttcgcccttt ccggaagctg tcgagggggg atcaattccc
      181 gatctagtaa catagatgac accgcgcgcg ataatttatc ctagtttgcg cgctatattt
      241 tgttttctat cgcgtattaa atgtataatt gcgggactct aatcataaaa acccatctca
      301 taaataacgt catgcattac atgttaatta ttacatgctt aacgtaattc aacagaaatt
      361 atatgataat catcgcaaga ccggcaacag gattcaatct taagaaactt tattgccaaa
      421 tgtttgaacg atcggacgtt tatcacaatt cctttttctt ttttgcctgg ccggcctttt
      481 tcgtggccgc cggccttttg tcgcctccca gctgagacag gtcgatccgt gtctcgtaca
      541 ggccggtgat gctctggtgg atcagggtgg cgtccagcac ctctttggtg ctggtgtacc
      601 tcttccggtc gatggtggtg tcaaagtact tgaaggcggc aggggctccc agattggtca
      661 gggtaaacag gtggatgata ttctcggcct gctctctgat gggcttatcc cggtgcttgt
      721 tgtaggcgga cagcactttg tccagattag cgtcggccag gatcactctc ttggagaact
      781 cgctgatctg ctcgatgatc tcgtccaggt agtgcttgtg ctgttccaca aacagctgtt
      841 tctgctcatt atcctcgggg gagcccttca gcttctcata gtggctggcc aggtacagga
      901 agttcacata tttggagggc agggccagtt cgtttccctt ctgcagttcg ccggcagagg
      961 ccagcattct cttccggccg ttttccagct cgaacaggga gtacttaggc agcttgatga
     1021 tcaggtcctt tttcactt
//
