LOCUS       sequence-124040-        1084 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  express in mammalian cells for unnatural amino acid incorporation 
            into VAMP2-GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Vreja IC, Nikic I, Gottfert F, Bates M, Krohnert K, Outeiro TF, Hell
            SW, Lemke EA, Rizzoli SO
  TITLE     Super-resolution Microscopy of Clickable Amino Acids Reveals the 
            Effects of Fluorescent Protein Tagging on Protein Assemblies.
  JOURNAL   ACS Nano. 2015 Nov 24;9(11):11034-41. doi: 10.1021/acsnano.5b04434. 
            Epub 2015 Oct 28.
  PUBMED    26498474
REFERENCE   2  (bases 1 to 1084)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acsnano.5b04434"; journalName: "ACS Nano"; date: 
            "2015-11-24- 24"; volume: "9"; issue: "11"; pages: "11034-41"
COMMENT     Sequence Label: pEGFP-N1 VAMP2 R125TAG
FEATURES             Location/Qualifiers
     source          1..1084
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      387..396
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(393..395,396..398,399..911)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     complement(438..459)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(699..718)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1046..1067
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ctaccgctgc caccgtcccg cctgccgccc cggccggcga gggtggcccc cctgcacctc
       61 ctccaaatct tactagtaac aggagactgc agcagaccca ggcccaggtg gatgaggtgg
      121 tggacatcat gagggtgaat gtggacaagg tcctggagcg ggaccagaag ttgtcggagc
      181 tggatgaccg tgcagatgcc ctccaggcag gggcctccca gtttgaaaca agtgcagcca
      241 agctcaagcg caaatactgg tggaaaaacc tcaagatgat gatcatcttg ggagtgatct
      301 gcgccatcat cctcatcatc atcatcgttt acttcagcac tcgaattctg cagtcgacgg
      361 taccgtaggc ccgggatcca ccggtcgcca ccatggtgag caagggcgag gagctgttca
      421 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
      481 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
      541 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc
      601 agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
      661 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
      721 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
      781 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
      841 acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc
      901 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
      961 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca
     1021 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
     1081 tcac
//
