LOCUS       sequence-124042-        1152 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  express in mammalian cells for unnatural amino acid incorporation 
            into VAMP2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1152)
  AUTHORS   Vreja IC, Nikic I, Gottfert F, Bates M, Krohnert K, Outeiro TF, Hell
            SW, Lemke EA, Rizzoli SO
  TITLE     Super-resolution Microscopy of Clickable Amino Acids Reveals the 
            Effects of Fluorescent Protein Tagging on Protein Assemblies.
  JOURNAL   ACS Nano. 2015 Nov 24;9(11):11034-41. doi: 10.1021/acsnano.5b04434. 
            Epub 2015 Oct 28.
  PUBMED    26498474
REFERENCE   2  (bases 1 to 1152)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acsnano.5b04434"; journalName: "ACS Nano"; date: 
            "2015-11-24- 24"; volume: "9"; issue: "11"; pages: "11034-41"
COMMENT     Sequence Label: pN1 VAMP2 R125TAG
FEATURES             Location/Qualifiers
     source          1..1152
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    546..667
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(583..602)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     637..656
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(811..832)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 cntnntntga cgtcagatcc gctagcgcta ccggactcag atctatgtcg gctaccgctg
       61 ccaccgtccc gcctgccgcc ccggccggcg agggtggccc ccctgcacct cctccaaatc
      121 ttactagtaa caggagactg cagcagaccc aggcccaggt ggatgaggtg gtggacatca
      181 tgagggtgaa tgtggacaag gtcctggagc gggaccagaa gttgtcggag ctggatgacc
      241 gtgcagatgc cctccaggca ggggcctccc agtttgaaac aagtgcagcc aagctcaagc
      301 gcaaatactg gtggaaaaac ctcaagatga tgatcatctt gggagtgatc tgcgccatca
      361 tcctcatcat catcatcgtt tacttcagca ctcgaattct gcagtcgacg gtaccgtagt
      421 aagcccggcc gcgactctag atcataatca gccataccac atttgtagag gttttacttg
      481 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg
      541 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
      601 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
      661 tatcttaagg cgtaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt
      721 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa
      781 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag
      841 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc aggcgatggc ccactacgtg
      901 aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc
      961 ctaaagggag cccccgattt agagcttgac gggaaagccn cgangtgnga gaaganggag
     1021 aagcgaaagg agcgggcgct aggcgctgca gtgtagcgnc acgctgcnnt aacaccacnn
     1081 cgcgngctan gcgncgctac ngnngtcagt gcactttnng annnnncgaa nncntnnntt
     1141 tnnnntcntc ga
//
