LOCUS       sequence-124046-       18440 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  a dCirl transcriptional reporter allele that contains an optimized 
            gal4.2::p65 cassette at the start codon of the genomic dCirl ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18440)
  AUTHORS   Scholz N, Gehring J, Guan C, Ljaschenko D, Fischer R, Lakshmanan V, 
            Kittel RJ, Langenhan T
  TITLE     The adhesion GPCR latrophilin/CIRL shapes mechanosensation.
  JOURNAL   Cell Rep. 2015 May 12;11(6):866-74. doi: 
            10.1016/j.celrep.2015.04.008. Epub 2015 Apr 30.
  PUBMED    25937282
REFERENCE   2  (bases 1 to 18440)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2015.04.008"; journalName: "Cell Rep"; date: 
            "2015-05-12- 12"; volume: "11"; issue: "6"; pages: "866-74"
COMMENT     Sequence Label: pTL464
FEATURES             Location/Qualifiers
     source          1..18440
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    join(1..31,32..233)
                     /label=P element 3' end
                     /note="P element 3' end"
     protein_bind    353..386
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     CDS             1982..2422
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     CDS             2867..3238
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
     primer_bind     complement(3507..3523)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3507..3523)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     regulatory      4273..4282
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    12735..12768
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    join(15812..16366,16367..16397)
                     /label=P element 5' end
     rep_origin      complement(16632..17220)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(16712..16731)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(17391..18182,18183..18251))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     18014..18033
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(18252..18356)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 catgatgaaa taacataagg tggtcccgtc ggcaagagac atccacttaa cgtatgcttg
       61 caataagtgc gagtgaaagg aatagtattc tgagtgtcgt attgagtctg agtgagacag
      121 cgatatgatt gttgattaac ccttagcatg tccgtggggt ttgaattaac tcataatatt
      181 aattagacga aattattttt aaagttttat ttttaataat ttgcgagtac gcaaagctag
      241 agttgttggt tggcacacca caaatatact gttgccgagc acaattccgc tcgaattgcc
      301 ggctgtacac ttaagaagtt cctactgcag gtaatgaatt gccgcggtgc gggtgccagg
      361 gcgtgccctt gggctccccg ggcgcgtact ccacgaattc ttgcatgcaa tgcggccgca
      421 gtaatttgtc ttcgatgtat gcataacaat caccacgtta cattcctatt tgaattgcgc
      481 gctcttccca gcagcaagtt ggcagtccga tagttgcgtt tttaggctgg caacaccctc
      541 cagcttcaac cagacattca agtatacaga atttttctaa aagaaagttg attgtgaatc
      601 gagctctcaa gcgaatagga tcgtctgcgt tgtatggagt ttaatccgaa atataagtgt
      661 aataattgcc caaagtgaag taaaacctag tgctaaccaa ggagcaaagc atccgtgaat
      721 aaaaaaaaac aaaaaagcaa cttaaagcca gtctttttcg tggctttggc atcatcaata
      781 actaagccaa caaatcgctc tgaaaatcgc caactggcga cagaaatcaa cgggaaaact
      841 tcattccgag tttaaaaatg ttttattctc gcggaattca agaaacgtcc acagttttca
      901 acgtgaaaaa aatgcccgga gttggaacaa agaaagacac ccaaactggg gctccgcggg
      961 gcagaaaaaa ctcgaagcgc agctgcagca acttttctct acatttacat ccacatgcat
     1021 atatgttcgt gaatagtgtg catcgggtgt acaaagaaaa cgggtctttg ttctgtccac
     1081 gaatcgatgg ccatgcaaca actacaacca cagtgtgggt gtacacagaa gggtgtgtat
     1141 tcttagctcc ctaccctacc ccctcccctg ccccatgcca tcttcttatc ttctatttgt
     1201 cttttgcctg cttgttcgtg tttgcccaag gagtcaatat gtaaacaatt atctaatgga
     1261 ttctctacct ttcagataca taagttgaat ggtacttttg agtccaactt tcatgaatat
     1321 gactccaaac ggaaatacat aagaggtatg tacatatgta cataggtgtg cttggcctgg
     1381 atatcatcat acataaatat aagaccattt taattattaa agatatataa atgcaattta
     1441 taattcttac aattaattat gttttcttga tatttgcatt gcttatatag tgttttaata
     1501 ggagttatac aaagaggttc tgaatattaa aataacttaa atttattaga atctacactt
     1561 ttgatattta ctgtaaccag gtcattagat tgactcaaca ttgatgtgat attgcacaag
     1621 taaattaaat ggaaccaata tcaataaaag aaaaggatag ttctcctttt cctttctaac
     1681 gaaatacaac atgtcttaag ttattagtta tgtgccttaa aataataata ataataatac
     1741 caaacattat aaatgttatc ttccctttca gtttgaatac ttcaaaccga gtgaaacact
     1801 gccgaatgag cattgccgat aagaaccaat gatggacgat cgatgattcg tgatctgggc
     1861 cggcaggatt tggtatttat gactactatt gcacttgcta ccaagctaac gacccacata
     1921 ctccaccatc aacctcagca ttactaagta tatcccaggc agtgtaaaaa ccggtatcaa
     1981 aatgaagctg ctgagtagta ttgaacaagc gtgtgacata tgccgactta agaagctgaa
     2041 gtgcagcaag gaaaagccga agtgcgccaa atgtcttaag aacaattggg agtgccggta
     2101 ctcgccaaag acgaagcgaa gccctttgac acgcgctcat ttgactgaag tggagagtcg
     2161 cctggagcgc cttgagcagt tgttcctcct catttttccc cgtgaagacc tcgacatgat
     2221 actgaagatg gatagtctcc aagatattaa ggccctgctc actggcctgt ttgttcagga
     2281 taacgtcaac aaggatgccg tgacggaccg ccttgcctcg gtggagacag atatgccact
     2341 taccttgcgg cagcatcgca tctcggctac cagcagtagt gaagaaagtt ccaataaggg
     2401 tcaacggcaa ctgacggtat cgggatccac gccgatggag ttccagtacc tgcccgatac
     2461 ggatgaccgt caccgtatcg aagaaaagcg gaagcgaacc tatgaaacct tcaagtccat
     2521 catgaaaaag tcccccttct cgggccccac ggacccgcgc cccccgcccc gtcgtattgc
     2581 ggttccttcg cgcagcagtg ccagcgtccc caaacccgca ccgcagccct acccgttcac
     2641 ttcctccctt agcacgatta actatgatga gttccccacg atggtgttcc ccagtggaca
     2701 aatttcccag gcatcggcac tggctccggc cccaccgcaa gtcctccccc aggcgcccgc
     2761 tccggcaccg gctcccgcaa tggtgagtgc tctggcccag gcccccgctc cagtccccgt
     2821 gctggcgcct ggacccccac aggcagttgc ccctcctgct ccgaaaccaa cgcaggcggg
     2881 cgaaggaacc ctgagcgagg ccctcttgca gcttcagttc gatgacgaag acttgggagc
     2941 cctgctgggt aacagcacag accctgccgt attcaccgat ctcgcatccg tggacaacag
     3001 cgagtttcag cagctcttga atcagggaat cccggtcgca cctcatacca cagagcccat
     3061 gctgatggaa tacccggagg ctatcacgcg actggtgacc ggcgcacagc gaccacccga
     3121 tccagcccct gccccactgg gtgccccggg tttgcccaat ggcctcctca gcggcgatga
     3181 ggatttctcc agcatcgctg atatggattt ctccgctttg ctgagccaga taagctccta
     3241 agtttaaaca agcttatcga taccgtcgac taaagccaaa tagaaaatta ttcagttcct
     3301 ggcttaagtt tttaaaagtg atattattta tttggttgta accaaccaaa agaatgtaaa
     3361 taactaatac ataattatgt tagttttaag ttagcaacaa attgatttta gctatattag
     3421 ctacttggtt aataaataga atatatttat ttaaagataa ttgcgttttt attgtcaggg
     3481 agtgagtttg cttaaaaact cgtttagatc cactagttct agagctagcg cgtatcatgc
     3541 taccaaccat attgtcaata tcctatgagc atatatcgtt agatcgtaag tgtctttgca
     3601 ggcccaccaa caatccaatc caaaccaaac caaactacaa caatcatcgc cgctaaccaa
     3661 aaaatttctt tttatatgcc atatatatat accatgtcca tataaaccca taccctgccc
     3721 agcaccctgt gacttatgaa gccttcgcct ttcagtgtcc aagtaccaaa ccgcctacgc
     3781 ctgcgaaggt aagaaactga ccatcgagtg cgatcccggc gatgtgatca acctcattcg
     3841 ggccaactat ggccgcttct cgattaccat ctgcaatgac cacgggaatg tggagtggag
     3901 tgttaactgc atgtttccca agtcactcag cgtactgaac tcaaggtaat acatctttta
     3961 aagtttattg gatcttcaaa tcattaaaca tgtcatttgg aaatttcata ctctggttgc
     4021 gaatatcaat aaactatttc aacacaagtc aatcaatcaa gcatcttaaa tatgtgaaaa
     4081 ttacccaact ctgtttactg atgaacgcgt atttttccca ttttccagat gtgcccacaa
     4141 gcagagctgc ggcgtgttgg cagccacgag catgttcggg gatccctgtc ccggtaccca
     4201 caagtatctg gaggcacact accagtgcat aagtgcagcc caaacttcga cgacgaccaa
     4261 caggcccagt ccgccgccat gggtgctgag caatggtccg ccgatctttg gcaacggcag
     4321 tggactgatc catccgcccg gggttggagc gggtgcgccg cccccgccga gacttcccac
     4381 acttcccgga gtggtgggaa tcagtgggaa tcccggcctg ttcaacgtac caccgcaaca
     4441 caccgccgtc acgcactcca cgccctcgag cagcacgaca gccgtgggcg gtggacgttt
     4501 gaagggtggg gccacctcca cgacgaccac caagcatccg gctggccgcc atgatggtct
     4561 gccaccgccg ccgcaactgc accaccacca caaccaccac ggtgaagaca ctgcctcacc
     4621 caccaagccg agcagcaagc tgccggctgg cggtaatgcc acttcaccat ccaacacgag
     4681 gatactcacg ggcgtcggag gttccggaac tgatgacgga accctactga ccacaaagag
     4741 ctcacccaac cgcccaccgg gcactgcggc cagtggatcc gttgtccccg ggaacggcag
     4801 cgtggtgcgc accatcaaca atattaattt gaacgcagcc gggatgtccg gaggcgatga
     4861 tgagtccaag ttgttttgcg gccccactca tgcccgcaat ttgtactgga acatgactcg
     4921 agtgggtgat gtgaatgttc agccctgtcc tggcggagca gccggcatcg ccaagtggcg
     4981 ttgcgttcta atgaagagga tacccgactc cggctacgat gagtacgatg atgacatcag
     5041 ttcgacaact ccggcaccca gcggtggcga ctgtctgcac aacagcagca gctgcgagcc
     5101 gccggtgagc atggcccaca aggtaaacca gcgtctgcgc aactttgagc ccacctggca
     5161 tcccgcgaca cctgatctga cgcaatgccg cagcctttgg ctcaacaatc tggaaatgcg
     5221 agtaaaccag cgggactcct ccttgatctc catcgccaac gatatgtccg aagtgaccag
     5281 tagcaaaacg ctctacggcg gcgacatgtt ggtcaccacg aagattatcc aaacagtgtc
     5341 cgagaagatg atgcacgaca aggagacctt cccggatcag cgacagcgcg aggctatgat
     5401 catggagttg ttgcattgtg tggtcaaaac cggctccaac ctgctggacg aatcgcagct
     5461 gtcctcgtgg ttggatctca atccggagga ccaaatgcgt gtagccacat ccttgctaac
     5521 tggcctggaa tacaatgcct ttctgctggc ggatacgatc atcagggagc gcagcgtggt
     5581 gcaaaaagtc aaaaatatat gtgagttcta aatgtttgca ttgtttgcat ttcaaaatac
     5641 ttataactgt atatcaccat agtgctctcc gttcgagttc tggaaaccaa gactatccag
     5701 tccagcgtgg tcttcccaga ttcggatcag tggcccttga gttcggatcg tattgagctg
     5761 ccacgagctg ctctaataga taatagtgaa ggcggtctgg tgcgaattgt attcgccgcc
     5821 ttcgatcgcc tggaatccat tctaaagccc agctatgatc acttcgatct caagagctcc
     5881 cgcagttacg gtaagtccac agagaactga aaaacgttag aatagtataa tctaaatgtt
     5941 ttcttagttc gcaatacagc catcctgagc aacgacagcg atgtcaacgc gggggagatc
     6001 caacagcgcc tacgcatcct gaacagcaag gtgatctcgg ccagcttggg caaggggcgt
     6061 cacatacaac tctcccagcc cataaccctg acactgaaac atctgaagac cgagaatgta
     6121 acgaatccca cctgcgtgtt ctggaactat attgaccagt aagactcata tgctcatatg
     6181 cctaataatt tatacgaatt aatgtttttg ttttagtgcg tggtctgcca acggatgcag
     6241 tctggagtcc actaaccgca cgcacagcgt ctgcagttgc aaccacctga caaactttgc
     6301 catactaatg gacgttgtgg atgagcacca gcattcgttg ttcaccatgt tcgatggaaa
     6361 catgcgcata ttcatctaca taagcatcgg catctgcgtg gtcttcatag ttatcgccct
     6421 gctaacgctg aagctgttca atggggtctt tgtgaaggta agaaacggct ccaatccctt
     6481 gccgcatcag cggtcgggca gcagacgcca gcaaaacaat attcgcgacc agacccacga
     6541 gtccttgacc ctgaccacgc caaccagtca gtccaatgtg ccgccgccca gtcatgggaa
     6601 cacgaacttt atccaacaca attccatccg caactcacac cgcaacaatc tgaactacaa
     6661 tgtccaacag cagcagcagc aacagcaaca acaagttgtt gcggcagctg ctgctgctgt
     6721 tgtgctgtcc aatcagccgc agcgaaatat gcaacatgcc atgaacaact tgaatctgaa
     6781 tctgcatcag cacggtcagc agacggctgc tgctgcagct gctgctgcct caatagccgc
     6841 tgcactgcag caacatgcag ttcaggccag caacgccagc aataacctca acatcagcca
     6901 caactatctg cagcagcagc atgtccagca gcagcagcag cagcagcgtg gccagcagcc
     6961 gcagccacat ccgcaccgca atcacaacct taatgtggac ggcaatggat tggataacac
     7021 gaacaacatc attatgcagg ccaacatgga cgacttacaa tataagtggt ggtggtggtg
     7081 aaaaaacaga taaacacaat tttaggctag gctaggcttt ctagacatat gtatttatga
     7141 ccgagagaca catgtgatat aatgcttaag taacttaaat tgttaatgaa cctctctctg
     7201 gcaggattca aagataagac tcaaaagctg tgctgggcag cgatgatcac tgttgcgtcc
     7261 cagtaacagc ttgcacgcga agactcccat ttatatttat gtatcgtagt aatttgtcat
     7321 agcttaagat tagcacacaa aaccaataat gaaatatctt catatgatag actcattttt
     7381 ctttttgtta caaatcatca ctctggcatg ttttacttaa tcgtttaatc atttttgaaa
     7441 taccataacc ccttttatgt gcattgattg tttaacaaat ttaacataga catcgtacaa
     7501 ctaaatacct agaacacttc tgactttctt ttgtatttca ttaattaata aaatatacaa
     7561 atgttgctaa ttttgtcctg taaatattgc attttgtgtt tgttcattta gtttgtatgt
     7621 atattaccta tgtgttgtgc ttgtttcgtg ttctgttcat tgttttattc gtattcgtat
     7681 tactcttagt ctttggattt tcaccaccac ccaccaaaat tattgtaaat caagttctaa
     7741 attaagtttg actatcaaat ctgacaccta ttcttcaaaa cgtttaagat tatcaatttc
     7801 cttaaaatgt ctaaaagctc cctgatgatc gaatctttag ggtccttctg ctggtgctgc
     7861 ttaaagaaaa aatttgcact gacttctcga acttatatta acttttcact ctgtcttctc
     7921 tttaactgtc cactccagtc cgcgcgcacc tcgatctata ccagcattta cctttgcctc
     7981 ctggccatcg agctgctctt tctcctgggc attgaacaga ccgaaacaag cattttctgc
     8041 ggcttcatta ctattttcct acactgtgcc atcctatcgg gcaccgcctg gttctgttac
     8101 gaaggtaaga aggccgaatt tcatctacac tttgcagccc taaccctttg cctctgcagc
     8161 cttccattcg tactcaacgc tcacctcgga cgagctcctg ctggaggtgg accagacgcc
     8221 caaggtgaac tgctactacc tcttgtccta cggactgtcg ctgagcgtgg tggccatctc
     8281 gctggtcatc gatcccagca cctataccca aaacgattat tgcgtgctga tggaggcgaa
     8341 tgccttgttt tatgccacct ttgtaatacc agtgcttgtc ttctttgtgg taagttacat
     8401 aatatgagat gaagcttcaa aagcgcagtt accattttaa attaattcac ttttacaggc
     8461 tgccattggt tacacattcc tctcctggat tataatgtgc cgcaaaagtc gcacgggtct
     8521 aaagaccaag gaacatactc gcctcgctag cgtgcggtaa gatattgaaa atccatatac
     8581 actttaaaga tccttcgatg cccatgtacc tcaaatccta ggttcgacat acgctgctcc
     8641 tttgtgttcc tcttgctgct cagcgctgtt tggtgctcgg cctacttcta tttgcgagga
     8701 gccaaaatgg acgatgacac ggctgatgtg tatggatact gcttcatctg cttcaacaca
     8761 ttgctggggc tctatatctt cgtgttccat tgcattcaaa acgaaaagat ccggcgggag
     8821 tatcggaagt atgtgagaca gcacgcttgg ctgcccaagt gcttgcgctg ctcgaaaaca
     8881 tcaatttcct cgggcattgt taccggcaat ggacccacag ccggaaccct ttgcagcgtc
     8941 tccacgtcca agaagcccaa gctgccgtta ggagtgagcg aagaggcgca tgacgatccc
     9001 cagcagcaac agcagacacc agtgcccatc acagaggatg ccattatggg agccacctct
     9061 gattgtgaac tgaacgaggc ccagcaaaga agaaccctaa aaagtggcct aatgacgggc
     9121 acactacagg ctccaccgca gacccttggt ggccatgttg tgctcgaaag aggtagcact
     9181 ctccgctcca ctggtcatgc ctcacccacc agctctgccg ggtccacaca cctgattttt
     9241 gcgcacaagc aacaacaaca acagcagcaa cagggacctt tgggcgagtc ttactaccat
     9301 cagccggact actacagctg gaagcaacca tcaactggaa caggaggatt gaaaacaccg
     9361 cgggagtact acaataatgc gggtgctgct gcatcatcgc cgcaacaggc gcacgaggta
     9421 ttctactgga ctcaaaagcc gaacagcggc cacaatggca aaaagaagag gggcgccgga
     9481 ggagttcccg cctcgcctag tggatctctg cacagtcgca cggccgccgc ctcccaggtg
     9541 cttttctatc catcgtacaa gaagaccaag cctggccagc caacaggcta tccgcaatac
     9601 gcggaggcgt tggacccacc actagccact ggcaatgcgg ctgcctacta ccagcagcag
     9661 caacagttgc gtcgccagca gctacatcag cagcagcaac agcagcagca gcagcaactc
     9721 tcctcggacg aggagcaggc cgagcaacat gctcacctgt tgcacctgca acgacgagct
     9781 ggtagccagc agcagctccc tgctccaccg ccacacatgg cgcagtacca gcaggagttt
     9841 atgcagcgcc agtatagaaa taagcattcc aactgtgatc tgggcatggg cgatgcctac
     9901 tacaaccaag gcagcgtcgg cggcgcggat ggtgggccgg tctacgagga gatcctcagc
     9961 aaccgcaact cggatgtgca gcattacgag gtgggtgact tcgatgtgga cgaggtgtac
    10021 aacaatagcg ttggcactgg cgtcttcaac aacatgagag cggcggtggc cgccggcggt
    10081 agtcggtacg gcggcggaag cctgagtggc ggcagtgtct cgtccaggag ccaacagcag
    10141 cagctcaaga agcagcagca acaacagtcg ctggctcagc aaagatcggc tcgacgctgc
    10201 acggcggatg acgatgacga cgaagacgag gaggaggatg aggaggcaac ggccgcggag
    10261 caattgcacg acagcgtctg tgatgaggat gaggaggagg acgagagcga cttggaggat
    10321 gatgctcatg gattgccacc gcagagcgat gagcggatgc gtcgtctgat ggcgatgcag
    10381 gacgaggatt ttaagcggcg gtttcagtaa gtacttctaa tgtttgacta tatcaacgtt
    10441 acgaaaaaat tacatttatc ccctttacag acgtcagctg cgcaaacatg gagcgcctct
    10501 tgactacggg gctttgccac caggagcagg accgcaaccc gagcacaacg gtgcggtttt
    10561 tggggttagc ggcggcgttg gtgagggctc cctgcgtggc gcattccggc agcagcagca
    10621 gcaagcactg aatgccaagt cgccaggcgg ccgtttggcg gtaaatgagc tattcggtca
    10681 cggcaactcg ggaccaccgc tgccgccggc gaatcagacg cccgcgcaga agcgccagca
    10741 gctacagaaa ctgtcaccac agtccaccac atcatcgtcg tcgcatacat cacacagcaa
    10801 tcccaatccg catccccacc agctaaccca tccgcatccc catcagcatc ctccgcacca
    10861 tcagcagcgc cacctgtcgg ccatgctcga tgagaacaac acggtgcgat gttatctgga
    10921 accactggct aagtgatcag ttaacgcaac tcccccggaa cagtcttagg caaaaattgt
    10981 agcttaggat ctctagctat acctagtatt agtcgttagt cgaaatatgg agcattgtgt
    11041 gaaacaacat gctgtcatgc tatacgtgca gtgattcgaa aatcccaaaa ctttataaat
    11101 gtatatgaaa taactaaaac ctagaccata gggcacatcc atacccctgc ggtgccgtgc
    11161 aagcttaacc aaaactcgag cgatgggggg ttggaattgg aattggaatc gggcccttcg
    11221 acttgcatgc attttatacc aacttggaaa tcgatattaa atataaatgt atgtaccata
    11281 tatgtatgat gtattgtaat acatgtcacc acatgccatt tttaaatact taaaaactaa
    11341 tttaaacaac cagaaaatca agtatatata tatggatgta ggtatatgta taagatctag
    11401 gaactgtatg tacatgatat atagaattca ggcgaattat gttttatgta ttttgtaaga
    11461 aatcaattgt ttttgattta cgctgattat gctcgtaaga tatatatata tatacacaca
    11521 ggcttaagat ttgttttgtg tttcataaac caaaaagata tcctacgttt tgaaagattg
    11581 tcatttcttg ctatccagct gccgttttcc acctatacaa ctaaaattcc ttccaactgc
    11641 gaatcaagag gaatataaga agtaaaggga aataatacat tgccattttg ttgctgcatt
    11701 tcagctacgt tgtaagcaaa ccagagcgaa gcatttgaag tgtacatttt catcaatcta
    11761 gatgctttaa gcacacacat aggggcattt actttatatt tttgagatac agagagagag
    11821 agagctcgcg aaatgcttta ttattttaca aaaccgatac agcatacgga aatgaaatac
    11881 ctatatagca aagaaagatt tcaaccaaac ctcacttgtt aactaggaac taactgaaaa
    11941 ctctttaaac actagccagc tgaaatccaa tcctaacact aaactgtact ctattcctac
    12001 tccccgaatg gcattctccc tagaatttca cgcatgtgta caaaaaccaa ataaaatata
    12061 gtgcaaacaa gatgattacc atatatgtcg tacataccaa gccaaggaag ctccgtgggg
    12121 gcagcaggcc cagctcgatc ccatttgatc tgatccgatt cgatccgatc cgatcagcaa
    12181 gttaaacttg aacgaaacat tagcacacaa tcgataaagt tagtaactaa gcacccgcag
    12241 catgccacac ataaagtgtc taaattggtt cggaaatcgc gtgtttgaaa gaggaataca
    12301 gaatagcaga tattgaatgt atagtgttgc gcctcctaga actttactta taaataataa
    12361 agcagattta tcagatacga gtatatatgt gcgtgtatat tgacgatgca agccattgca
    12421 atccccctct tttgtcgcca cattctcgcg aaattgattc tagacctagg cattgaattg
    12481 ataaatcaga gattttgaat aaagcagaaa ccgaaactga aaaccagacg cccaaatcaa
    12541 agttcataaa aagcccgatg aaaatgttaa tgtacacaat tttttctatt taagacaaaa
    12601 tacaagaaat ggattgcatt ttactgtaaa tgacaaataa gtgttttctt gggatattgg
    12661 gttcaaaatt taaaacgggt gtttaattta aatagtttgc tttcaaaaat ggctttaaat
    12721 ggcgcgccgg taccataact tcgtataatg tatgctatac gaagttatcg tacgactagc
    12781 acctgactgt cgagcccagt ggaaaccctt gaaatgcctt taagtcgagc ccagtggaaa
    12841 cccttgaaat gcctttaagt cgagcccagt ggaaaccctt gaaatgcctt taagtcgagc
    12901 ccagtggaaa cccttgaaat gcctttaagt cgagcccagt ggaaaccctt gaaatgcctt
    12961 taagtcgagt agcagtcgag gtaggtacgc catgccccgt tattctctat tcgttttgtg
    13021 actctccctc tctgtactat tgctctctca ctctgtcgca cagtaaacgg cacgctattc
    13081 tcgttgcttc gagagagcgc gcctcgaatg ttcgcgaaaa gagcgccgga gtataaatag
    13141 aggcgcttcg tcgacggagc gtcaattcaa ttcaaacaag caaagtgaac acatcgcgaa
    13201 gcaagctgag caaacaaaca agcgcagctg aacaagctaa acaatctgca ataaagtgca
    13261 agttaaagtg aatcaattaa aagtaaccaa caaccaagta attaaactaa aaactgcaac
    13321 tactgaaatc aaccaagaag tcattattga agacaagaag agaactctga ataggtcgat
    13381 agcgtcaatt cggcacgagg tttcgtgacg aagctccaag cggtttacgc catcaattaa
    13441 acacaaagtg ctgtgccaaa actcctctcg cttcttattt ttgtttgttt tttgagtgat
    13501 tgggtgggtg attggttttg ggtgggtaag caggggaaag tgtgaaaaat cccggcaatg
    13561 ggccaagagg atcaggagct attaattcgc ggaggcagca aacacccatc tgccgagcat
    13621 ctgaacaatg gtgacagcgg agcggcttcg cagagctgca ttaaccaggg cttcgggcag
    13681 gccaaaaact acggcacgct cctgccaccc agtccgccgg aggactccgg ttcagggagc
    13741 ggccaactag ccgagaacct cacctatgcc tggcacaata tggacatctt tggggcggtc
    13801 aatcagccgg gctccggatg gcggcagctg gtcaaccgga cacgcggact attctgcaac
    13861 gagcgacaca taccggcgcc caggaaacat ttgctcaaga acgtttgcgg cgtggcctat
    13921 ccgggcgaac ttttggccgt gatgggcagt tccggtgccg gaaagacgac cctgctgaat
    13981 gcccttgcct ttcgatcgcc gcagggcatc caagtatcgc catccgggat gcgactgctc
    14041 aatggccaac ctgtggacgc caaggagatg caggccaggt gcgcctatgt ccagcaggat
    14101 gacctcttta tcggctccct aacggccagg gaacacctga ttttccaggc catggtgcgg
    14161 atgccacgac atctgaccta tcggcagcga gtggcccgcg tggatcaggt gatccaggag
    14221 ctttcgctca gcaaatgtca gcacacgatc atcggtgtgc ccggcagggt gaaaggtctg
    14281 tccggcggag aaaggaagcg tctggcattc gcctccgagg cactaaccga tccgccgctt
    14341 ctgatctgcg atgagcccac ctccggactg gactcattta ccgcccacag cgtcgtccag
    14401 gtgctgaaga agctgtcgca gaagggcaag accgtcatcc tgaccattca tcagccgtct
    14461 tccgagctgt ttgagctctt tgacaagatc cttctgatgg ccgagggcag ggtagctttc
    14521 ttgggcactc ccagcgaagc cgtcgacttc ttttcctacg tgggtgccca gtgtcctacc
    14581 aactacaatc cggcggactt ttacgtacag gtgttggccg ttgtgcccgg acgggagatc
    14641 gagtcccgtg atcggatcgc caagatatgc gacaattttg ccattagcaa agtagcccgg
    14701 gatatggagc agttgttggc caccaaaaat ttggagaagc cactggagca gccggagaat
    14761 gggtacacct acaaggccac ctggttcatg cagttccggg cggtcctgtg gcgatcctgg
    14821 ctgtcggtgc tcaaggaacc actcctcgta aaagtgcgac ttattcagac aacgatggtt
    14881 gccatcttga ttggcctcat ctttttgggc caacaactca cgcaagtggg cgtgatgaat
    14941 atcaacggag ccatcttcct cttcctgacc aacatgacct ttcaaaacgt ctttgccacg
    15001 ataaatgtgt tcacctcaga gctgccagtt tttatgaggg aggcccgaag tcgactttat
    15061 cgctgtgaca catactttct gggcaaaacg attgccgaat tgccgctttt tctcacagtg
    15121 ccactggtct tcacggcgat tgcctatccg atgatcggac tgcgggccgg agtgctgcac
    15181 ttcttcaact gcctggcgct ggtcactctg gtggccaatg tgtcaacgtc cttcggatat
    15241 ctaatatcct gcgccagctc ctcgacctcg atggcgctgt ctgtgggtcc gccggttatc
    15301 ataccattcc tgctctttgg cggcttcttc ttgaactcgg gctcggtgcc agtatacctc
    15361 aaatggttgt cgtacctctc atggttccgt tacgccaacg agggtctgct gattaaccaa
    15421 tgggcggacg tggagccggg cgaaattagc tgcacatcgt cgaacaccac gtgccccagt
    15481 tcgggcaagg tcatcctgga gacgcttaac ttctccgccg ccgatctgcc gctggactac
    15541 gtgggtctgg ccattctcat cgtgagcttc cgggtgctcg catatctggc tctaagactt
    15601 cgggcccgac gcaaggagta gccgacatat atccgaaata actgtttgtt tttttttttt
    15661 accattatta ccatcgtgtt tactgtttat tgccccctca aaaagctaat gtaattatat
    15721 ttgtgccaat aaaaacaaga tatgacctat agaatacaaa aaaaaaaaaa aaaaaaaaaa
    15781 aaaaactcga caccgggatc caaacctagt ggctgctgct ctaaacgacg catttcgtac
    15841 tccaaagtac gaattttttc cctcaagctc ttattttcat taaacaatga acaggaccta
    15901 acgcacagtc acgttattgt ttacataaat gatttttttt actattcaaa cttactctgt
    15961 ttgtgtactc ccactggtat agccttcttt tatcttttct ggttcaggct ctatcacttt
    16021 actaggtacg gcatctgcgt tgagtcgcct ccttttaaat gtctgacctt ttgcaggtgc
    16081 agccttccac tgcgaatcat taaagtgggt atcacaaatt tgggagtttt caccaaggct
    16141 gcacccaagg ctctgctccc acaattttct cttaatagca cacttcggca cgtgaattaa
    16201 ttttactcca gtcacagctt tgcagcaaaa tttgcaatat ttcatttttt tttattccac
    16261 gtaagggtta atgttttcaa aaaaaaattc gtccgcacac aacctttcct ctcaacaagc
    16321 aaacgtgcac tgaatttaag tgtatacttc ggtaagcttc ggctatcgac gggaccacct
    16381 tatgttattt catcatgggc cagacccacg tagtccagcg gcagatcggc ggcggagaag
    16441 ttaagcgtct ccaggatgac cttgcccgaa ctggggcacg tggtgttcga cgatgtgcag
    16501 ctaatttcgc ccggctccac gtccgcccat tggttaatca gcagaccctc gttggcgtaa
    16561 cggaaccatg agaggtacga caaccatttg aggtatactg gcaccgagcc cgagttcaag
    16621 aagaaggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
    16681 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
    16741 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
    16801 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
    16861 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
    16921 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
    16981 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
    17041 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
    17101 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
    17161 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
    17221 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
    17281 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
    17341 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
    17401 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
    17461 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
    17521 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
    17581 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
    17641 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
    17701 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
    17761 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
    17821 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
    17881 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
    17941 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
    18001 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
    18061 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
    18121 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
    18181 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
    18241 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
    18301 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
    18361 tttccccgaa aagtgccacc tgcgaagtct tagagccaga tatgcgagca cccggaagct
    18421 cacgatgaga atggccagac
//
