LOCUS       sequence-124048-        8179 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8179)
  AUTHORS   Li L, Zhang L, Zhang X, Yan Q, Minamishima YA, Olumi AF, Mao M, 
            Bartz S, Kaelin WG
  TITLE     Hypoxia-inducible factor linked to differential kidney cancer risk 
            seen with type 2A and type 2B VHL mutations.
  JOURNAL   Mol Cell Biol. 2007 Aug . 27(15):5381-92.
  PUBMED    17526729
REFERENCE   2  (bases 1 to 8179)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2007 Aug . 27(15):5381-92."
COMMENT     Sequence Label: HA-HIF2-alpha P405A/P531A-pBabe-Hygro
FEATURES             Location/Qualifiers
     source          1..8179
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             98..567
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    632..831
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1024..1440
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1408..1424
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(4099..4119)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4104..4433
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4284..4419
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4507..5532
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             4507..5532
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     LTR             6327..6796
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     CDS             complement(join(7281..8072,8073..8141))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7904..7923
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(8142..8179,1..67))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
       61 ttccgcgcac atttccccga aaagtgccac ctgcagcctg aatatgggcc aaacaggata
      121 tctgtggtaa gcagttcctg ccccggctca gggccaagaa cagatggaac agctgaatat
      181 gggccaaaca ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat
      241 ggtccccaga tgcggtccag ccctcagcag tttctagaga accatcagat gtttccaggg
      301 tgccccaagg acctgaaatg accctgtgcc ttatttgaac taaccaatca gttcgcttct
      361 cgcttctgtt cgcgcgcttc tgctccccga gctcaataaa agagcccaca acccctcact
      421 cggggcgcca gtcctccgat tgactgagtc gcccgggtac ccgtgtatcc aataaaccct
      481 cttgcagttg catccgactt gtggtctcgc tgttccttgg gagggtctcc tctgagtgat
      541 tgactacccg tcagcggggg tctttcattt gggggctcgt ccgggatcgg gagacccctg
      601 cccagggacc accgacccac caccgggagg caagctggcc agcaacttat ctgtgtctgt
      661 ccgattgtct agtgtctatg actgatttta tgcgcctgcg tcggtactag ttagctaact
      721 agctctgtat ctggcggacc cgtggtggaa ctgacgagtt ctgaacaccc ggccgcaacc
      781 ctgggagacg tcccagggac tttgggggcc gtttttgtgg cccgacctga ggaagggagt
      841 cgatgtggaa tccgaccccg tcaggatatg tggttctggt aggagacgag aacctaaaac
      901 agttcccgcc tccgtctgaa tttttgcttt cggtttggaa ccgaagccgc gcgtcttgtc
      961 tgctgcagca tcgttctgtg ttgtctctgt ctgactgtgt ttctgtattt gtctgaaaat
     1021 tagggccaga ctgttaccac tcccttaagt ttgaccttag atcactggaa agatgtcgag
     1081 cggctcgctc acaaccagtc ggtagatgtc aagaagagac gttgggttac cttctgctct
     1141 gcagaatggc caacctttaa cgtcggatgg ccgcgagacg gcacctttaa ccgagacctc
     1201 atcacccagg ttaagatcaa ggtcttttca cctggcccgc atggacaccc agaccaggtc
     1261 ccctacatcg tgacctggga agccttggct tttgaccccc ctccctgggt caagcccttt
     1321 gtacacccta agcctccgcc tcctcttctt ccatccgcgc cgtctctccc ccttgaacct
     1381 cctctttcga ccccgcctca atcctccctt tatccagccc tcactccttc tctaggcgcc
     1441 ggccggatcc gccaccatga cagctgacaa ggagaagaaa aggagtagct cggagaggag
     1501 gaaggagaag tcccgggatg ctgcgcggtg ccggcggagc aaggagacgg aggtgttcta
     1561 tgagctggcc catgagctgc ctctgcccca cagtgtgagc tcccatctgg acaaggcctc
     1621 catcatgcga ctggcaatca gcttcctgcg aacacacaag ctcctctcct cagtttgctc
     1681 tgaaaacgag tccgaagccg aagctgacca gcagatggac aacttgtacc tgaaagcctt
     1741 ggagggtttc attgccgtgg tgacccaaga tggcgacatg atctttctgt cagaaaacat
     1801 cagcaagttc atgggactta cacaggtgga gctaacagga catagtatct ttgacttcac
     1861 tcatccctgc gaccatgagg agattcgtga gaacctgagt ctcaaaaatg gctctggttt
     1921 tgggaaaaaa agcaaagaca tgtccacaga gcgggacttc ttcatgagga tgaagtgcac
     1981 ggtcaccaac agaggccgta ctgtcaacct caagtcagcc acctggaagg tcttgcactg
     2041 cacgggccag gtgaaagtct acaacaactg ccctcctcac aatagtctgt gtggctacaa
     2101 ggagcccctg ctgtcctgcc tcatcatcat gtgtgaacca atccagcacc catcccacat
     2161 ggacatcccc ctggatagca agaccttcct gagccgccac agcatggaca tgaagttcac
     2221 ctactgtgat gacagaatca cagaactgat tggttaccac cctgaggagc tgcttggccg
     2281 ctcagcctat gaattctacc atgcgctaga ctccgagaac atgaccaaga gtcaccagaa
     2341 cttgtgcacc aagggtcagg tagtaagtgg ccagtaccgg atgctcgcaa agcatggggg
     2401 ctacgtgtgg ctggagaccc aggggacggt catctacaac cctcgcaacc tgcagcccca
     2461 gtgcatcatg tgtgtcaact acgtcctgag tgagattgag aagaatgacg tggtgttctc
     2521 catggaccag actgaatccc tgttcaagcc ccacctgatg gccatgaaca gcatctttga
     2581 tagcagtggc aagggggctg tgtctgagaa gagtaacttc ctattcacca agctaaagga
     2641 ggagcccgag gagctggccc agctggctgc taccccagga gacgccatca tctctctgga
     2701 tttcgggaat cagaacttcg aggagtcctc agcctatggc aaggccatcc tgcccccgag
     2761 ccagccatgg gccacggagt tgaggagcca cagcacccag agcgaggctg ggagcctgcc
     2821 tgccttcacc gtgccccagg cagctgcccc gggcagcacc acccccagtg ccaccagcag
     2881 cagcagcagc tgctccacgc ccaatagccc tgaagactat tacacatctt tggataacga
     2941 cctgaagatt gaagtgattg agaagctctt cgccatggac acagaggcca aggaccaatg
     3001 cagtacccag acggatttca atgagctgga cttggagaca ctggcagctt atatccccat
     3061 ggacggggaa gacttccagc taagccccat ctgccccgag gagcggctct tggcggagaa
     3121 cccacagtcc accccccagc actgcttcag tgccatgaca aacatcttcc agccactggc
     3181 ccctgtagcc ccgcacagtc ccttcctcct ggacaagttt cagcagcagc tggagagcaa
     3241 gaagacagag cccgagcacc ggcccatgtc ctccatcttc tttgatgccg gaagcaaagc
     3301 atccctgcca ccgtgctgtg gccaggccag cacccctctc tcttccatgg ggggcagatc
     3361 caatacccag tggcccccag atccaccatt acattttggg cccacaaagt gggccgtcgg
     3421 ggatcagcgc acagagttct tgggagcagc gccgttgggg ccccctgtct ctccacccca
     3481 tgtctccacc ttcaagacaa ggtctgcaaa gggttttggg gctcgaggcc cagacgtgct
     3541 gagtccggcc atggtagccc tctccaacaa gctgaagctg aagcgacagc tggagtatga
     3601 agagcaagcc ttccaggacc tgagcggggg ggacccacct ggtggcagca cctcacattt
     3661 gatgtggaaa cggatgaaga acctcagggg tgggagctgc cctttgatgc cggacaagcc
     3721 actgagcgca aatgtaccca atgataagtt cacccaaaac cccatgaggg gcctgggcca
     3781 tcccctgaga catctgccgc tgccacagcc tccatctgcc atcagtcccg gggagaacag
     3841 caagagcagg ttccccccac agtgctacgc cacccagtac caggactaca gcctgtcgtc
     3901 agcccacaag gtgtcaggca tggcaagccg gctgctcggg ccctcatttg agtcctacct
     3961 gctgcccgaa ctgaccagat atgactgtga ggtgaacgtg cccgtgctgg gaagctccac
     4021 gctcctgcaa ggaggggacc tcctcagagc cctggaccag gccacctgac aattcgccag
     4081 cacagtggtc gaccctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
     4141 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
     4201 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     4261 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     4321 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
     4381 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagcttg
     4441 ccagctgggg cgccctctgg taaggttggg aagccctgca gatctgatcc cggggggcaa
     4501 tgagatatga aaaagcctga actcaccgcg acgtctgtcg agaagtttct gatcgaaaag
     4561 ttcgacagcg tctccgacct gatgcagctc tcggagggcg aagaatctcg tgctttcagc
     4621 ttcgatgtag gagggcgtgg atatgtcctg cgggtaaata gctgcgccga tggtttctac
     4681 aaagatcgtt atgtttatcg gcactttgca tcggccgcgc tcccgattcc ggaagtgctt
     4741 gacattgggg aatttagcga gagcctgacc tattgcatct cccgccgtgc acagggtgtc
     4801 acgttgcaag acctgcctga aaccgaactg cccgctgttc tgcagccggt cgcggaggcc
     4861 atggatgcga tcgctgcggc cgatcttagc cagacgagcg ggttcggccc attcggaccg
     4921 caaggaatcg gtcaatacac tacatggcgt gatttcatat gcgcgattgc tgatccccat
     4981 gtgtatcact ggcaaactgt gatggacgac accgtcagtg cgtccgtcgc gcaggctctc
     5041 gatgagctga tgctttgggc cgaggactgc cccgaagtcc ggcacctcgt gcacgcggat
     5101 ttcggctcca acaatgtcct gacggacaat ggccgcataa cagcggtcat tgactggagc
     5161 gaggcgatgt tcggggattc ccaatacgag gtcgccaaca tcttcttctg gaggccgtgg
     5221 ttggcttgta tggagcagca gacgcgctac ttcgagcgga ggcatccgga gcttgcagga
     5281 tcgccgcggc tccgggcgta tatgctccgc attggtcttg accaactcta tcagagcttg
     5341 gttgacggca atttcgatga tgcagcttgg gcgcagggtc gatgcgacgc aatcgtccga
     5401 tccggagccg ggactgtcgg gcgtacacaa atcgcccgca gaagcgcggc cgtctggacc
     5461 gatggctgtg tagaagtact cgccgatagt ggaaaccgac gccccagcac tcgtccgagg
     5521 gcaaaggaat agagtagatg ccgaccggga tctatcgatg cggcgggtgt ggtggttacg
     5581 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     5641 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     5701 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     5761 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     5821 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     5881 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     5941 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     6001 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg aaacgcctgg
     6061 tatcttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt tgtgatgctc
     6121 gtcagggggg cggagcctat ggaaaaacgc cagcaacgca tcgataaaat aaaagatttt
     6181 atttagtctc cagaaaaagg ggggaatgaa agaccccacc tgtaggtttg gcaagctagc
     6241 ttaagtaacg ccattttgca aggcatggaa aaatacataa ctgagaatag agaagttcag
     6301 atcaaggtca ggaacagatg gaacagctga atatgggcca aacaggatat ctgtggtaag
     6361 cagttcctgc cccggctcag ggccaagaac agatggaaca gctgaatatg ggccaaacag
     6421 gatatctgtg gtaagcagtt cctgccccgg ctcagggcca agaacagatg gtccccagat
     6481 gcggtccagc cctcagcagt ttctagagaa ccatcagatg tttccagggt gccccaagga
     6541 cctgaaatga ccctgtgcct tatttgaact aaccaatcag ttcgcttctc gcttctgttc
     6601 gcgcgcttct gctccccgag ctcaataaaa gagcccacaa cccctcactc ggggcgccag
     6661 tcctccgatt gactgagtcg cccgggtacc cgtgtatcca ataaaccctc ttgcagttgc
     6721 atccgacttg tggtctcgct gttccttggg agggtctcct ctgagtgatt gactacccgt
     6781 cagcgggggt ctttcacatg cagcatgtat caaaattaat ttggtttttt ttcttaagta
     6841 tttacattaa atggccatag tgcggccgca aatcaatcta aagtatatat gagtaaactt
     6901 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     6961 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     7021 catctggccc cccaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     7081 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttacgctc
     7141 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     7201 cctagatcct tttaaattaa aaatgaagtt tgcggccgca aatcaatcta aagtatatat
     7261 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     7321 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     7381 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     7441 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     7501 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     7561 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     7621 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     7681 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     7741 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     7801 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     7861 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac gggataatac cgcgccacat
     7921 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     7981 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     8041 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     8101 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     8161 tattgaagca tttatcagg
//
