LOCUS       sequence-124049-       15674 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  dCirl promoter region was replaced with an optimized 20xUAS-IVS 
            promoter cassette .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15674)
  AUTHORS   Scholz N, Gehring J, Guan C, Ljaschenko D, Fischer R, Lakshmanan V, 
            Kittel RJ, Langenhan T
  TITLE     The adhesion GPCR latrophilin/CIRL shapes mechanosensation.
  JOURNAL   Cell Rep. 2015 May 12;11(6):866-74. doi: 
            10.1016/j.celrep.2015.04.008. Epub 2015 Apr 30.
  PUBMED    25937282
REFERENCE   2  (bases 1 to 15674)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2015.04.008"; journalName: "Cell Rep"; date: 
            "2015-05-12- 12"; volume: "11"; issue: "6"; pages: "866-74"
COMMENT     Sequence Label: pTL472
FEATURES             Location/Qualifiers
     source          1..15674
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        31..135
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(136..204,205..996)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(354..373)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1167..1755
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1656..1675
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    1842..1911
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     primer_bind     complement(2136..2154)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(2606..2677,3079..3352,3427..4081,4143..4458,4662..4793,
                     4864..5478)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    6508..6602
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    6636..6730
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    6764..6858
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    6892..6986
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        7011..7249
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     7196..7217
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     intron          7264..7330
                     /label=Mhc intron
                     /note="modified Drosophila myosin heavy chain intron 16 
                     (Pfeiffer et al., 2010)"
     regulatory      8105..8114
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             12399..12464
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     intron          14957..15022
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             15152..15172
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    15444..15578
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(15481..15500)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     15535..15554
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(15620..15638)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gactcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
       61 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
      121 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
      181 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
      241 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
      301 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
      361 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
      421 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
      481 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
      541 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
      601 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
      661 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
      721 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
      781 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
      841 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
      901 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
      961 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     1021 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     1081 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     1141 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     1201 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     1261 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     1321 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     1381 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     1441 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     1501 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     1561 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     1621 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     1681 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     1741 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     1801 ccttttgctc acatgttacc gtcgacgatg taggtcacgg tctcgaagcc gcggtgcggg
     1861 tgccagggcg tgcccttggg ctccccgggc gcgtactcca cctcacccat ctggtccatc
     1921 atgatgaacg ggtcgaggtg gcggtagttg atcccggcga acgcgcggcg caccgggaag
     1981 ccctcgccct cgaaaccgct gggcgcggtg gtcacggtga gcacgggacg tgcgacggcg
     2041 tcggcgggtg cggatacgcg gggcagcgtc agcgggttct cgacggtcac ggcgggcatg
     2101 tcgacaagcc gaacatatgg gcgcgcctag tatgtatgta agttaataaa acccattttt
     2161 gcggaaagta gataaaaaaa acattttttt tttttactgc actggatatc attgaactta
     2221 tctgatcagt tttaaattta cttcgatcca agggtatttg atgtaccagg ttctttcgat
     2281 tacctctcac tcaaaatgac attccactca aagtcagcgc tgtttgcctc cttctctgtc
     2341 cacagaaata tcgccgtctc tttcgccgct gcgtccgcta tctctttcgc caccgtttgt
     2401 agcgttacgt agcgtcaatg tccgccttca gttgcatttt gtcagcggtt tcgtgacgaa
     2461 gctccaagcg gtttacgcca tcaattaaac acaaagtgct gtgccaaaac tcctctcgct
     2521 tcttattttt gtttgttttt tgagtgattg gggtggtgat tggttttggg tgggtaagca
     2581 ggggaaagtg tgaaaaatcc cggcaatggg ccaagaggat caggagctat taattcgcgg
     2641 aggcagcaaa cacccatctg ccgagcatct gaacaatgtg agtagtacat gtgcatacat
     2701 cttaagttca cttgatctat aggaactgcg attgcaacat caaattgtat gcggcgtgag
     2761 aactgcgacc cacaaaaatc ccaaaccgca attgcacaaa caaatagtga cacgaaacag
     2821 attattctgg tagctgttct cgctatataa gacaattttt gagatcatat catgatcaag
     2881 acatctaaag gcattcattt tcgactatat tcttttttac aaaaaatata acaaccagat
     2941 attttaagct gatcctagat gcacaaaaaa taaataaaag tataaaccta cttcgtagga
     3001 tacttcgggg tactttttgt tcggggttag atgagcataa cgctagtagt tgatatttga
     3061 gatcccctat cattgcaggg tgacagcgga gcggcttcgc agagctgcat taaccagggc
     3121 ttcgggcagg ccaaaaacta cggcacgctc cggccaccca gtccgccgga ggactccggt
     3181 tcagggagcg gccaactagc cgagaacctc acctatgcct ggcacaatat ggacatcttt
     3241 ggggcggtca atcagccggg ctccggatgg cggcagctgg tcaaccggac acgcggacta
     3301 ttctgcaacg agcgacacat accggcgccc aggaaacatt tgctcaagaa cggtgagttt
     3361 ctattcgcag tcggctgatc tgtgtgaaat cttaataaag ggtccaatta ccaatttgaa
     3421 actcagtttg cggcgtggcc tatccgggcg aacttttggc cgtgatgggc agttccggtg
     3481 ccggaaagac gaccctgctg aatgcccttg cctttcgatc gccgcagggc atccaagtat
     3541 cgccatccgg gatgcgactg ctcaatggcc aacctgtgga cgccaaggag atgcaggcca
     3601 ggtgcgccta tgtccagcag gatgacctct ttatcggctc cctaacggcc agggaacacc
     3661 tgattttcca agccatggtg cggatgccac gacatctgac ctatcggcag cgagtggccc
     3721 gcgtggatca ggtgatccag gagctttcgc tcagcaaatg tcagcacacg atcatcggtg
     3781 tgcccggcag ggtgaaaggt ctgtccggcg gagaaaggaa gcgtctggca ttcgcctccg
     3841 aggctctaac cgatccgccg cttctgatct gcgatgagcc cacctccgga ctggactcct
     3901 ttaccgccca cagcgtcgtc caggtgctga agaagctgtc gcagaagggc aagaccgtca
     3961 tcctgaccat tcatcagccg tcttccgagc tgtttgagct ctttgacaag atccttctga
     4021 tggccgaggg cagggtagct ttcttgggca ctcccagcga agccgtcgac ttcttttcct
     4081 agtgagttcg atgtgtttat taagggtatc tagtattaca taacatctca actcctatcc
     4141 agcgtgggtg cccagtgtcc taccaactac aatccggcgg acttttacgt acaggtgttg
     4201 gccgttgtgc ccggacggga gatcgagtcc cgtgatcgga tcgccaagat atgcgacaat
     4261 tttgccatta gcaaagtagc ccgggatatg gagcagttgt tggccaccaa aaatctggag
     4321 aagccactgg agcagccgga gaatgggtac acctacaagg ccacctggtt catgcagttc
     4381 cgggcggtcc tgtggcgatc ctggctgtcg gtgctcaagg aaccactcct cgtaaaagtg
     4441 cgacttattc agacaacggt gagtggttcc agtggaaaca aatgatataa cgcttacaat
     4501 tcttggaaac aaattcgcta gattttagat agaattgcct gattccacac ccttcttagt
     4561 ttttttcaat gagatgtata gtttatagtt ttgcagaaga taaataaatt tcatttaact
     4621 cgcgaatatt aatgagatgc gagtaacatt ttaatttgca gatggttgcc atcttgattg
     4681 gcctcatctt tttgggccaa caactcacgc aagtgggtgt gatgaatatc aacggagcca
     4741 tcttcctctt cctgaccaac atgacctttc aaaacgtctt tgccacgata aatgtaagtc
     4801 atgtttagaa tacatttgca tttcaataat ttactaactt tctaatgaat cgattcgatt
     4861 taggtgttca cctcagagct gccagttttt atgagggagg cccgaagtcg actttatcgc
     4921 tgtgacacat actttctggg caaaacgatt gccgaattgc cgctttttct cacagtgcca
     4981 ctggtcttca cggcgattgc ctatccgatg atcggactgc gggccggagt gctgcacttc
     5041 ttcaactgcc tggcgctggt cactctggtg gccaatgtgt caacgtcctt cggatatcta
     5101 atatcctgcg ccagctcctc gacctcgatg gcgctgtctg tgggtccgcc ggttatcata
     5161 ccattcctgc tctttggcgg cttcttcttg aactcgggct cggtgccagt atacctcaaa
     5221 tggttgtcgt acctctcatg gttccgttac gccaacgagg gtctgctgat taaccaatgg
     5281 gcggacgtgg agccgggcga aattagctgc acatcgtcga acaccacgtg ccccagttcg
     5341 ggcaaggtca tcctggagac gcttaacttc tccgccgccg atctgccgct ggactacgtg
     5401 ggtctggcca ttctcatcgt gagcttccgg gtgctcgcat atctggctct aagacttcgg
     5461 gcccgacgca aggagtagcc gacatatatc cgaaataact gcttgttttt tttttttacc
     5521 attattacca tcgtgtttac tgtttattgc cccctcaaaa agctaatgta attatatttg
     5581 tgccaataaa aacaagatat gacctataga atacaagtat ttccccttcg aacatcccca
     5641 caagtagact ttggatttgt cttctaacca aaagacttac acacctgcat accttacatc
     5701 aaaaactcgt ttatcgctac ataaaacacc gggatatatt ttttatatac atacttttca
     5761 aatcgcgcgc cctcttcata attcacctcc accacaccac gtttcgtagt tgctctttcg
     5821 ctgtctccca cccgctctcc gcaacacatt caccttttgt tcgacgacct tggagcgact
     5881 gtcgttagtt ccgcgcgatt cggttcgctc aaatggttcc gagtggttca tttcgtctca
     5941 atagaaatta gtaataaata tttgtatgta caatttattt gctccaatat atttgtatat
     6001 atttccctca cagctatatt tattctaatt taatattatg actttttaag gtaatttttt
     6061 gtgacctgtt cggagtgatt agcgttacaa tttgaactga aagtgacatc cagtgtttgt
     6121 tccttgtgta gatgcatctc aaaaaaatgg tgggcataat agtgttgttt atatatatca
     6181 aaaataacaa ctataataat aagaatacat ttaatttaga aaatgcttgg atttcactgg
     6241 aactagggcg cgcctccgga acataatggt gcagggcgct gacttccgcg tttccagact
     6301 ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca
     6361 gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca
     6421 accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca
     6481 gggccgcaag cttgcatgcc tgcaggtcgg agtactgtcc tccgagcgga gtactgtcct
     6541 ccgagcggag tactgtcctc cgagcggagt actgtcctcc gagcggagta ctgtcctccg
     6601 agcggagact ctagccctag ggcatgcctg caggtcggag tactgtcctc cgagcggagt
     6661 actgtcctcc gagcggagta ctgtcctccg agcggagtac tgtcctccga gcggagtact
     6721 gtcctccgag cggagactct agcgctagcg catgcctgca ggtcggagta ctgtcctccg
     6781 agcggagtac tgtcctccga gcggagtact gtcctccgag cggagtactg tcctccgagc
     6841 ggagtactgt cctccgagcg gagactctag cactagtgca tgcctgcagg tcggagtact
     6901 gtcctccgag cggagtactg tcctccgagc ggagtactgt cctccgagcg gagtactgtc
     6961 ctccgagcgg agtactgtcc tccgagcgga gactctagcg acgtcgagcg ccggagtata
     7021 aatagaggcg cttcgtctac ggagcgacaa ttcaattcaa acaagcaaag tgaacacgtc
     7081 gctaagcgaa agctaagcaa ataaacaagc gcagctgaac aagctaaaca atctgcagta
     7141 aagtgcaagt taaagtgaat caattaaaag taaccagcaa ccaagtaaat caactgcaac
     7201 tactgaaatc tgccaagaag taattattga atacaagaag agaactctga atagatctaa
     7261 aaggtaggtt caaccactga tgcctaggca caccgaaacg actaacccta attcttatcc
     7321 tttacttcag gcggccgcag tatatcccag gcagtgtaaa agcgtatcat gctaccaacc
     7381 atattgtcaa tatcctatga gcatatatcg ttagatcgta agtgtctttg caggcccacc
     7441 aacaatccaa tccaaaccaa accaaactac aacaatcatc gccgctaacc aaaaaatttc
     7501 tttttatatg ccatatatat ataccatgtc catataaacc cataccctgc ccagcaccct
     7561 gtgacttatg aagccttcgc ctttcagtgt ccaagtacca aaccgcctac gcctgcgaag
     7621 gtaagaaact gaccatcgag tgcgatcccg gcgatgtgat caacctcatt cgggccaact
     7681 atggccgctt ctcgattacc atctgcaatg accacgggaa tgtggagtgg agtgttaact
     7741 gcatgtttcc caagtcactc agcgtactga actcaaggta atacatcttt taaagtttat
     7801 tggatcttca aatcattaaa catgtcattt ggaaatttca tactctggtt gcgaatatca
     7861 ataaactatt tcaacacaag tcaatcaatc aagcatctta aatatgtgaa aattacccaa
     7921 ctctgtttac tgatgaacgc gtatttttcc cattttccag atgtgcccac aagcagagct
     7981 gcggcgtgtt ggcagccacg agcatgttcg gggatccctg tcccggtacc cacaagtatc
     8041 tggaggcaca ctaccagtgc ataagtgcag cccaaacttc gacgacgacc aacaggccca
     8101 gtccgccgcc atgggtgctg agcaatggtc cgccgatctt tggcaacggc agtggactga
     8161 tccatccgcc cggggttgga gcgggtgcgc cgcccccgcc gagacttccc acacttcccg
     8221 gagtggtggg aatcagtggg aatcccggcc tgttcaacgt accaccgcaa cacaccgccg
     8281 tcacgcactc cacgccctcg agcagcacga cagccgtggg cggtggacgt ttgaagggtg
     8341 gggccacctc cacgacgacc accaagcatc cggctggccg ccatgatggt ctgccaccgc
     8401 cgccgcaact gcaccaccac cacaaccacc acggtgaaga cactgcctca cccaccaagc
     8461 cgagcagcaa gctgccggct ggcggtaatg ccacttcacc atccaacacg aggatactca
     8521 cgggcgtcgg aggttccgga actgatgacg gaaccctact gaccacaaag agctcaccca
     8581 accgcccacc gggcactgcg gccagtggat ccgttgtccc cgggaacggc agcgtggtgc
     8641 gcaccatcaa caatattaat ttgaacgcag ccgggatgtc cggaggcgat gatgagtcca
     8701 agttgttttg cggccccact catgcccgca atttgtactg gaacatgact cgagtgggtg
     8761 atgtgaatgt tcagccctgt cctggcggag cagccggcat cgccaagtgg cgttgcgttc
     8821 taatgaagag gatacccgac tccggctacg atgagtacga tgatgacatc agttcgacaa
     8881 ctccggcacc cagcggtggc gactgtctgc acaacagcag cagctgcgag ccgccggtga
     8941 gcatggccca caaggtaaac cagcgtctgc gcaactttga gcccacctgg catcccgcga
     9001 cacctgatct gacgcaatgc cgcagccttt ggctcaacaa tctggaaatg cgagtaaacc
     9061 agcgggactc ctccttgatc tccatcgcca acgatatgtc cgaagtgacc agtagcaaaa
     9121 cgctctacgg cggcgacatg ttggtcacca cgaagattat ccaaacagtg tccgagaaga
     9181 tgatgcacga caaggagacc ttcccggatc agcgacagcg cgaggctatg atcatggagt
     9241 tgttgcattg tgtggtcaaa accggctcca acctgctgga cgaatcgcag ctgtcctcgt
     9301 ggttggatct caatccggag gaccaaatgc gtgtagccac atccttgcta actggcctgg
     9361 aatacaatgc ctttctgctg gcggatacga tcatcaggga gcgcagcgtg gtgcaaaaag
     9421 tcaaaaatat atgtgagttc taaatgtttg cattgtttgc atttcaaaat acttataact
     9481 gtatatcacc atagtgctct ccgttcgagt tctggaaacc aagactatcc agtccagcgt
     9541 ggtcttccca gattcggatc agtggccctt gagttcggat cgtattgagc tgccacgagc
     9601 tgctctaata gataatagtg aaggcggtct ggtgcgaatt gtattcgccg ccttcgatcg
     9661 cctggaatcc attctaaagc ccagctatga tcacttcgat ctcaagagct cccgcagtta
     9721 cggtaagtcc acagagaact gaaaaacgtt agaatagtat aatctaaatg ttttcttagt
     9781 tcgcaataca gccatcctga gcaacgacag cgatgtcaac gcgggggaga tccaacagcg
     9841 cctacgcatc ctgaacagca aggtgatctc ggccagcttg ggcaaggggc gtcacataca
     9901 actctcccag cccataaccc tgacactgaa acatctgaag accgagaatg taacgaatcc
     9961 cacctgcgtg ttctggaact atattgacca gtaagactca tatgctcata tgcctaataa
    10021 tttatacgaa ttaatgtttt tgttttagtg cgtggtctgc caacggatgc agtctggagt
    10081 ccactaaccg cacgcacagc gtctgcagtt gcaaccacct gacaaacttt gccatactaa
    10141 tggacgttgt ggatgagcac cagcattcgt tgttcaccat gttcgatgga aacatgcgca
    10201 tattcatcta cataagcatc ggcatctgcg tggtcttcat agttatcgcc ctgctaacgc
    10261 tgaagctgtt caatggggtc tttgtgaagg taagaaacgg ctccaatccc ttgccgcatc
    10321 agcggtcggg cagcagacgc cagcaaaaca atattcgcga ccagacccac gagtccttga
    10381 ccctgaccac gccaaccagt cagtccaatg tgccgccgcc cagtcatggg aacacgaact
    10441 ttatccaaca caattccatc cgcaactcac accgcaacaa tctgaactac aatgtccaac
    10501 agcagcagca gcaacagcaa caacaagttg ttgcggcagc tgctgctgct gttgtgctgt
    10561 ccaatcagcc gcagcgaaat atgcaacatg ccatgaacaa cttgaatctg aatctgcatc
    10621 agcacggtca gcagacggct gctgctgcag ctgctgctgc ctcaatagcc gctgcactgc
    10681 agcaacatgc agttcaggcc agcaacgcca gcaataacct caacatcagc cacaactatc
    10741 tgcagcagca gcatgtccag cagcagcagc agcagcagcg tggccagcag ccgcagccac
    10801 atccgcaccg caatcacaac cttaatgtgg acggcaatgg attggataac acgaacaaca
    10861 tcattatgca ggccaacatg gacgacttac aatataagtg gtggtggtgg tgaaaaaaca
    10921 gataaacaca attttaggct aggctaggct ttctagacat atgtatttat gaccgagaga
    10981 cacatgtgat ataatgctta agtaacttaa attgttaatg aacctctctc tggcaggatt
    11041 caaagataag actcaaaagc tgtgctgggc agcgatgatc actgttgcgt cccagtaaca
    11101 gcttgcacgc gaagactccc atttatattt atgtatcgta gtaatttgtc atagcttaag
    11161 attagcacac aaaaccaata atgaaatatc ttcatatgat agactcattt ttctttttgt
    11221 tacaaatcat cactctggca tgttttactt aatcgtttaa tcatttttga aataccataa
    11281 ccccttttat gtgcattgat tgtttaacaa atttaacata gacatcgtac aactaaatac
    11341 ctagaacact tctgactttc ttttgtattt cattaattaa taaaatatac aaatgttgct
    11401 aattttgtcc tgtaaatatt gcattttgtg tttgttcatt tagtttgtat gtatattacc
    11461 tatgtgttgt gcttgtttcg tgttctgttc attgttttat tcgtattcgt attactctta
    11521 gtctttggat tttcaccacc acccaccaaa attattgtaa atcaagttct aaattaagtt
    11581 tgactatcaa atctgacacc tattcttcaa aacgtttaag attatcaatt tccttaaaat
    11641 gtctaaaagc tccctgatga tcgaatcttt agggtccttc tgctggtgct gcttaaagaa
    11701 aaaatttgca ctgacttctc gaacttatat taacttttca ctctgtcttc tctttaactg
    11761 tccactccag tccgcgcgca cctcgatcta taccagcatt tacctttgcc tcctggccat
    11821 cgagctgctc tttctcctgg gcattgaaca gaccgaaaca agcattttct gcggcttcat
    11881 tactattttc ctacactgtg ccatcctatc gggcaccgcc tggttctgtt acgaaggtaa
    11941 gaaggccgaa tttcatctac actttgcagc cctaaccctt tgcctctgca gccttccatt
    12001 cgtactcaac gctcacctcg gacgagctcc tgctggaggt ggaccagacg cccaaggtga
    12061 actgctacta cctcttgtcc tacggactgt cgctgagcgt ggtggccatc tcgctggtca
    12121 tcgatcccag cacctatacc caaaacgatt attgcgtgct gatggaggcg aatgccttgt
    12181 tttatgccac ctttgtaata ccagtgcttg tcttctttgt ggtaagttac ataatatgag
    12241 atgaagcttc aaaagcgcag ttaccatttt aaattaattc acttttacag gctgccattg
    12301 gttacacatt cctctcctgg attataatgt gccgcaaaag tcgcacgggt ctaaagacca
    12361 aggaacatac tcgcctcgct agcgctgcgg ctgcagctga ctacaaagac catgacggtg
    12421 attataaaga tcatgacatc gattacaagg atgacgatga caaggctgct gcagcggcta
    12481 gcgtgcggta agatattgaa aatccatata cactttaaag atccttcgat gcccatgtac
    12541 ctcaaatcct aggttcgaca tacgctgctc ctttgtgttc ctcttgctgc tcagcgctgt
    12601 ttggtgctcg gcctacttct atttgcgagg agccaaaatg gacgatgaca cggctgatgt
    12661 gtatggatac tgcttcatct gcttcaacac attgctgggg ctctatatct tcgtgttcca
    12721 ttgcattcaa aacgaaaaga tccggcggga gtatcggaag tatgtgagac agcacgcttg
    12781 gctgcccaag tgcttgcgct gctcgaaaac atcaatttcc tcgggcattg ttaccggcaa
    12841 tggacccaca gccggaaccc tttgcagcgt ctccacgtcc aagaagccca agctgccgtt
    12901 aggagtgagc gaagaggcgc atgacgatcc ccagcagcaa cagcagacac cagtgcccat
    12961 cacagaggat gccattatgg gagccacctc tgattgtgaa ctgaacgagg cccagcaaag
    13021 aagaacccta aaaagtggcc taatgacggg cacactacag gctccaccgc agacccttgg
    13081 tggccatgtt gtgctcgaaa gaggtagcac tctccgctcc actggtcatg cctcacccac
    13141 cagctctgcc gggtccacac acctgatttt tgcgcacaag caacaacaac aacagcagca
    13201 acagggacct ttgggcgagt cttactacca tcagccggac tactacagct ggaagcaacc
    13261 atcaactgga acaggaggat tgaaaacacc gcgggagtac tacaataatg cgggtgctgc
    13321 tgcatcatcg ccgcaacagg cgcacgaggt attctactgg actcaaaagc cgaacagcgg
    13381 ccacaatggc aaaaagaaga ggggcgccgg aggagttccc gcctcgccta gtggatctct
    13441 gcacagtcgc acggccgccg cctcccaggt gcttttctat ccatcgtaca agaagaccaa
    13501 gcctggccag ccaacaggct atccgcaata cgcggaggcg ttggacccac cactagccac
    13561 tggcaatgcg gctgcctact accagcagca gcaacagttg cgtcgccagc agctacatca
    13621 gcagcagcaa cagcagcagc agcagcaact ctcctcggac gaggagcagg ccgagcaaca
    13681 tgctcacctg ttgcacctgc aacgacgagc tggtagccag cagcagctcc ctgctccacc
    13741 gccacacatg gcgcagtacc agcaggagtt tatgcagcgc cagtatagaa ataagcattc
    13801 caactgtgat ctgggcatgg gcgatgccta ctacaaccaa ggcagcgtcg gcggcgcgga
    13861 tggtgggccg gtctacgagg agatcctcag caaccgcaac tcggatgtgc agcattacga
    13921 ggtgggtgac ttcgatgtgg acgaggtgta caacaatagc gttggcactg gcgtcttcaa
    13981 caacatgaga gcggcggtgg ccgccggcgg tagtcggtac ggcggcggaa gcctgagtgg
    14041 cggcagtgtc tcgtccagga gccaacagca gcagctcaag aagcagcagc aacaacagtc
    14101 gctggctcag caaagatcgg ctcgacgctg cacggcggat gacgatgacg acgaagacga
    14161 ggaggaggat gaggaggcaa cggccgcgga gcaattgcac gacagcgtct gtgatgagga
    14221 tgaggaggag gacgagagcg acttggagga tgatgctcat ggattgccac cgcagagcga
    14281 tgagcggatg cgtcgtctga tggcgatgca ggacgaggat tttaagcggc ggtttcagta
    14341 agtacttcta atgtttgact atatcaacgt tacgaaaaaa ttacatttat cccctttaca
    14401 gacgtcagct gcgcaaacat ggagcgcctc ttgactacgg ggctttgcca ccaggagcag
    14461 gaccgcaacc cgagcacaac ggtgcggttt ttggggttag cggcggcgtt ggtgagggct
    14521 ccctgcgtgg cgcattccgg cagcagcagc agcaagcact gaatgccaag tcgccaggcg
    14581 gccgtttggc ggtaaatgag ctattcggtc acggcaactc gggaccaccg ctgccgccgg
    14641 cgaatcagac gcccgcgcag aagcgccagc agctacagaa actgtcacca cagtccacca
    14701 catcatcgtc gtcgcataca tcacacagca atcccaatcc gcatccccac cagctaaccc
    14761 atccgcatcc ccatcagcat cctccgcacc atcagcagcg ccacctgtcg gccatgctcg
    14821 atgagaacaa cacggtgcga tgttatctgg aaccactggc taagtgaacc ggtactagag
    14881 gatctttgtg aaggaacctt acttctgtgg tgtgacataa ttggacaaac tacctacaga
    14941 gatttaaagc tctaaggtaa atataaaatt tttaagtgta taatgtgtta aactactgat
    15001 tctaattgtt tgtgtatttt agattccaac ctatggaact gatgaatggg agcagtggtg
    15061 gaatgccttt aatgaggaaa acctgttttg ctcagaagaa atgccatcta gtgatgatga
    15121 ggctactgct gactctcaac attctactcc tccaaaaaag aagagaaagg tagaagaccc
    15181 caaggacttt ccttcagaat tgctaagttt tttgagtcat gctgtgttta gtaatagaac
    15241 tcttgcttgc tttgctattt acaccacaaa ggaaaaagct gcactgctat acaagaaaat
    15301 tatggaaaaa tatttgatgt atagtgcctt gactagagat cataatcagc cataccacat
    15361 ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata
    15421 aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa
    15481 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
    15541 tgtccaaact catcaatgta tcttatcatg tctggatcga tctggccggc cgtttaaacg
    15601 aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
    15661 ataatggttt ctta
//
