LOCUS       sequence-124055-         750 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  redox-sensitive red fluorescent protein rxRFP1.1, nuclear 
            localization.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Fan Y, Ai HW
  TITLE     Development of redox-sensitive red fluorescent proteins for imaging 
            redox dynamics in cellular compartments.
  JOURNAL   Anal Bioanal Chem. 2016 Jan 12.
  PUBMED    26758595
REFERENCE   2  (bases 1 to 750)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Anal 
            Bioanal Chem. 2016 Jan 12."
COMMENT     Sequence Label: pNuc-rxRFP1.1
FEATURES             Location/Qualifiers
     source          1..750
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             21..41
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             45..65
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     promoter        complement(105..123)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(106..123)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(143..160)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    149..373
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(506..525)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     716..737
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ggtgcaaaag tgtggctgat ccaaaaaaga agagaaaggt agacccgaag aaaaaacgaa
       61 aggtagatcc aaagaagaag agaaagtaat ctagagggcc ctattctata gtgtcaccta
      121 aatgctagag ctcgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
      181 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
      241 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
      301 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
      361 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac
      421 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
      481 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
      541 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
      601 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
      661 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
      721 ctcttgttcc aaactggaac aacactcaac
//
