LOCUS       sequence-124066-        1428 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Human CDK8 W105M Mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1428)
  AUTHORS   Pelish HE, Liau BB, Nitulescu II, Tangpeerachaikul A, Poss ZC, Da 
            Silva DH, Caruso BT, Arefolov A, Fadeyi O, Christie AL, Du K, Banka 
            D, Schneider EV, Jestel A, Zou G, Si C, Ebmeier CC, Bronson RT, 
            Krivtsov AV, Myers AG, Kohl NE, Kung AL, Armstrong SA, Lemieux ME, 
            Taatjes DJ, Shair MD
  TITLE     Mediator kinase inhibition further activates 
            super-enhancer-associated genes in AML.
  JOURNAL   Nature. 2015 Sep 28. doi: 10.1038/nature14904.
  PUBMED    26416749
REFERENCE   2  (bases 1 to 1428)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Sep 28. doi: 10.1038/nature14904."
COMMENT     Sequence Label: pLVX-Flag-CDK8-W105M-IRES-mCherry
FEATURES             Location/Qualifiers
     source          1..1428
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..27
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atggactaca aagacgatga cgacaagaga tctatggact atgactttaa agtgaagctg
       61 agcagcgagc gggagcgggt cgaggacctg tttgaatacg agggctgcaa agttggccga
      121 ggcacttatg gtcacgtcta caaagccaag aggaaagatg ggaaggatga taaagactat
      181 gctttaaaac aaatagaagg aactgggatc tctatgtcgg catgtagaga aatagcatta
      241 cttcgagagc ttaagcatcc aaacgtcatt tctcttcaaa aggtgtttct gtctcatgct
      301 gataggaagg tgtggcttct gtttgactat gctgaacatg acctcatgca tataatcaag
      361 tttcacagag cttctaaagc aaacaagaag ccagttcagt tacctcgggg aatggtgaag
      421 tcactattat atcagatcct agatggtatt cactacctgc atgctaactg ggtgttgcac
      481 agagatttga aacctgctaa tattttagtt atgggtgaag gtcctgagcg aggaagagta
      541 aaaattgctg acatgggctt tgcccgatta tttaattcac ctttgaagcc tttagcagat
      601 ttggatccag tggttgttac attctggtac cgagcccctg aactacttct tggagcaagg
      661 cattatacca aagctattga tatttgggct atagggtgta tatttgcaga actactaacg
      721 tcagaaccaa tatttcactg tcgacaagag gacatcaaaa ctagtaatcc ttatcaccat
      781 gaccagctgg acagaatatt caatgtaatg ggatttcctg cagataaaga ttgggaagat
      841 ataaaaaaga tgcctgaaca ttcaacatta atgaaagatt tcagaagaaa tacgtatacc
      901 aactgcagcc ttatcaagta tatggaaaaa cataaagtta aaccagatag taaagcattc
      961 cacttgcttc agaagctgct taccatggac ccaataaagc gaattacctc agaacaggct
     1021 atgcaggacc cctatttctt agaagaccca cttcctacat cagacgtttt tgccggttgt
     1081 caaatccctt acccaaaacg agaattttta acggaagaag aacctgatga caaaggagac
     1141 aaaaagaacc agcagcagca gcagggcaat aaccacacta atggaactgg ccacccaggg
     1201 aatcaagaca gcagtcacac acagggaccc ccgttgaaga aagtgagagt tgttcctcct
     1261 accactacct caggtggact tatcatgacc tcagactatc agcgttccaa tccacatgct
     1321 gcctatccca accctggacc aagcacatca cagccgcaga gcagcatggg atactcagct
     1381 acctcccagc agcctccaca gtactcacat cagacacatc ggtactga
//
