LOCUS       sequence-124108-        1154 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 462, Emission: 492.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1154)
  AUTHORS   Ai HW, Olenych SG, Wong P, Davidson MW, Campbell RE
  TITLE     Hue-shifted monomeric variants of Clavularia cyan fluorescent 
            protein: identification of the molecular determinants of color and 
            applications in fluorescence imaging.
  JOURNAL   BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/1741-7007-6-13.
  PUBMED    18325109
REFERENCE   2  (bases 1 to 1154)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Biol.";
            date: "2008-03-6"; volume: "6:13. doi"; pages: " 
            10.1186/1741-7007-6-13"
COMMENT     Sequence Label: mTFP1-H4-23
FEATURES             Location/Qualifiers
     source          1..1154
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    338..390
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      398..407
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             404..1114
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
ORIGIN
        1 gacgtcagat ccgctagccg ccaccatgtc tggccgcggc aaaggcggga agggtcttgg
       61 caaaggcggc gctaagcgcc accgtaaagt actgcgcgac aatatccagg gcatcaccaa
      121 gccggccatc cggcgccttg ctcgccgcgg cggcgtgaag cgcatctccg gcctcatcta
      181 cgaggagact cgcggggtgc tgaaggtgtt cctggagaac gtgatccggg acgccgtgac
      241 ctatacagag cacgccaagc gcaagacggt caccgccatg gatgtggtct acgcgctcaa
      301 gcgccagggc cgcaccctct acggtttcgg tggggatctc gagctcaagc ttcgaattct
      361 gcagtcgacg gtaccgcggc cccgggatcc accggtcgcc accatggtga gcaagggcga
      421 ggagaccaca atgggcgtaa tcaagcccga catgaagatc aagctgaaga tggagggcaa
      481 cgtgaatggc cacgccttcg tgatcgaggg cgagggcgag ggcaagccct acgacggcac
      541 caacaccatc aacctggagg tgaaggaggg agcccccctg cccttctcct acgacattct
      601 gaccaccgcg ttcgcctacg gcaacagggc cttcaccaag taccccgacg acatccccaa
      661 ctacttcaag cagtccttcc ccgagggcta ctcttgggag cgcaccatga ccttcgagga
      721 caagggcatc gtgaaggtga agtccgacat ctccatggag gaggactcct tcatctacga
      781 gatacacctc aagggcgaga acttcccccc caacggcccc gtgatgcaga agaagaccac
      841 cggctgggac gcctccaccg agaggatgta cgtgcgcgac ggcgtgctga agggcgacgt
      901 caagcacaag ctgctgctgg agggcggcgg ccaccaccgc gttgacttca agaccatcta
      961 cagggccaag aaggcggtga agctgcccga ctatcacttt gtggaccacc gcatcgagat
     1021 cctgaaccac gacaaggact acaacaaggt gaccgtttac gagagcgccg tggcccgcaa
     1081 ctccaccgac ggcatggacg agctgtacaa gtaagcggcc gcgactctag atcataatca
     1141 gccataccac attg
//
