LOCUS       sequence-124112-        5099 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  N1 invertor plasmid for two-strain system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5099)
  AUTHORS   Chen Y, Kim JK, Hirning AJ, Josic K, Bennett MR
  TITLE     SYNTHETIC BIOLOGY. Emergent genetic oscillations in a synthetic 
            microbial consortium.
  JOURNAL   Science. 2015 Aug 28;349(6251):986-9. doi: 10.1126/science.aaa3794.
  PUBMED    26315440
REFERENCE   2  (bases 1 to 5099)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2015-08-28- 28"; volume: "349"; 
            issue: "6251"; pages: "986-9"
COMMENT     Sequence Label: pC235
FEATURES             Location/Qualifiers
     source          1..5099
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    873..891
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    903..921
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             complement(1075..1104)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(1295..1317)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1306..1322
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      2061..2088
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             2263..3141
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     CDS             complement(3267..4058)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATYDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     primer_bind     complement(4239..4256)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4373..4918)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(4453..4472)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4990..5009
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 ttattacgct gcaagggcgt aattttcgtc gttcgctgct atatattccg ggaacactct
       61 acaactcttt tcctgctcta tatcatgacc aaagaaaata attggtttct cttttttcac
      121 aacttctttt aaacgtttaa ttgaagataa agctaattct ggatcaaatc ctgcgaacgg
      181 cacttcatct tcaaaattct ctttcgtgta cgatgcatca atcgttaata aaactgaacc
      241 ggattgctcc gtctcaatga atagcgactg atggcctgga gaatgacctg gcgtatacaa
      301 taattgaaca cctggtacca cttcataatc cccttcaata attttgtagt tcaaatgcgg
      361 taatatacat tctttcatat attcttctct atgaagtgct gcctcatatt ccgttcgctg
      421 cacaataatt ggtgtatttg taaaagcacc gtttcctcct gcatgatcaa aatgtaagtg
      481 agaactaata atatataaaa ggtcgtccgg ctcataccct acacgcttta atatattcac
      541 gattctatct tcttcagtca ttttcggtaa gatctgtcct tcaacaaatg taccgttaaa
      601 aagcccttca ttattaactg cactttctgg catacctgtg tctactaaaa taggaccttc
      661 ttccgtctcc aaaagataac accacaccgg caagtttaat agtttccccg gtgttaacgc
      721 actgttaaca gacgaatgat ccaacatgca acgacctgct gggatgaaat aaagtttctt
      781 tactgtcata ctagtatttc tccttagcat gattaagatg tttcagtacg aaaattgctt
      841 tcattgttga tctccttttt aagtggcatg catctctatc actgataggg atgtgctcat
      901 tatctctatc actgataggg aagtaaattc cctcagatag gcccccccag atgaggggga
      961 tcagagcatg tcgcgcaaaa gtgtgcagcg gttttgcgat aacgacatgc gccacgcaca
     1021 agagttccgt agctgttcaa gtttgtgttt caactgttct cgtcgtttcc gcaacaagtc
     1081 ctcttcagaa atgagctttt gctcctctgc ttggacggac aggatgtatg ctgtggcttt
     1141 tttaaggata actaccttgg gggccttttc attgttttcc aactccggga tctggtcacg
     1201 cagggcaaaa aagctccgtt ttagctcgtt cctcctctgg cgctccaaga cgttgtgtgt
     1261 tcgcctcttg acattctcct cggtgtccga gggccctgtg tgaaattgtt atccgctcac
     1321 aattccacac agacgtctta acttgcttcc tagtgaaacc gttcaggaca tctacgggat
     1381 tgttgctaag aaagtcaacg agattctaca agcagacgca atcaatggga ccgataacga
     1441 agtagttacc gtgaccgatg agaacactgg tgaaatctct gagaaagtca agctgggcac
     1501 taaggcactg gctggtcaat ggctggctta cggtgttact cgcagtgtga ctaagcgttc
     1561 agtcatgacg ctggcttacg ggtccaaaga gttcggcttc cgtcaacaag tgctggaaga
     1621 taccattcag ccagctattg attccggcaa gggtctgatg ttcactcagc cgaatcaggc
     1681 tgctggatac atggctaagc tgatttggga atctgtgagc gtgacggtgg tagctgcggt
     1741 tgaagcaatg aactggctta agtctgctgc taagctgctg gctgctgagg tcaaagataa
     1801 gaagactgga gagattcttc gcaagcgttg cgctgtgcat tgggtaactc ctgatggttt
     1861 ccctgtgtgg caggaataca agaagcctat tcagacgcgc ttctcgagta ataaaagctt
     1921 cccctacgtg ctgctgaagt tgcccgcaac agagagtgga accaaccggt gataccacga
     1981 tactatgact gagagtcaac gccatgagcg gcctcatttc ttattctgag ttacaacagt
     2041 ccgcaccgct gtctcacact ggctcacctt cgggtgggcc tttctgcgtt tatatactag
     2101 agagagaata taaaaagcca gattattaat ccggcttttt tattatttcc tagcggccgc
     2161 ggtttggtta gcgagaagag ccagtaaaag acgcagtgac ggcaatgtct gatgcaatat
     2221 ggacaattgg tttcttctct gaatggcggg agtatgaaaa gtatggctga agcgcaaaat
     2281 gatcccctgc tgccgggata ctcgtttaat gcccatctgg tggcgggttt aacgccgatt
     2341 gaggccaacg gttatctcga tttttttatc gaccgaccgc tgggaatgaa aggttatatt
     2401 ctcaatctca ccattcgcgg tcagggggtg gtgaaaaatc agggacgaga atttgtttgc
     2461 cgaccgggtg atattttgct gttcccgcca ggagagattc atcactacgg tcgtcatccg
     2521 gaggctcgcg aatggtatca ccagtgggtt tactttcgtc cgcgcgccta ctggcatgaa
     2581 tggcttaact ggccgtcaat atttgccaat acggggttct ttcgcccgga tgaagcgcac
     2641 cagccgcatt tcagcgacct gtttgggcaa atcattaacg ccgggcaagg ggaagggcgc
     2701 tattcggagc tgctggcgat aaatctgctt gagcaattgt tactgcggcg catggaagcg
     2761 attaacgagt cgctccatcc accgatggat aatcgggtac gcgaggcttg tcagtacatc
     2821 agcgatcacc tggcagacag caattttgat atcgccagcg tcgcacagca tgtttgcttg
     2881 tcgccgtcgc gtctgtcaca tcttttccgc cagcagttag ggattagcgt cttaagctgg
     2941 cgcgaggacc aacgtatcag ccaggcgaag ctgcttttga gcaccacccg gatgcctatc
     3001 gccaccgtcg gtcgcaatgt tggttttgac gatcaactct atttctcgcg ggtatttaaa
     3061 aaatgcaccg gggccagccc gagcgagttc cgtgccggtt gtgaagaaaa agtgaatgat
     3121 gtagccgtca agttgtcata attggtaacg aatcagacaa ttgacggctt gacggagtag
     3181 catagggttt gcagaatccc tgcttcgtcc atttgacagg cacattatgc tagcgatatc
     3241 cgtcggcttg aacgaattgt tagacattat ttgccgacta ccttggtgat ctcgcctttc
     3301 acgtagtgga caaattcttc caactgatct gcgcgcgagg ccaagcgatc ttcttcttgt
     3361 ccaagataag cctgtctagc ttcaagtatg acgggctgat actgggccgg caggcgctcc
     3421 attgcccagt cggcagcgac atccttcggc gcgattttgc cggttactgc gctgtaccaa
     3481 atgcgggaca acgtaagcac tacatttcgc tcatcgccag cccagtcggg cggcgagttc
     3541 catagcgtta aggtttcatt tagcgcctca aatagatcct gttcaggaac cggatcaaag
     3601 agttcctccg ccgctggacc taccaaggca acgctatgtt ctcttgcttt tgtcagcaag
     3661 atagccagat caatgtcgta cgtggctggc tcgaagatac ctgcaagaat gtcattgcgc
     3721 tgccattctc caaattgcag ttcgcgctta gctggataac gccacggaat gatgtcgtcg
     3781 tgcacaacaa tggtgacttc tacagcgcgg agaatctcgc tctctccagg ggaagccgaa
     3841 gtttccaaaa ggtcgttgat caaagctcgc cgcgttgttt catcaagcct tacggtcacc
     3901 gtaaccagca aatcaatatc actgtgtggc ttcaggccgc catccactgc ggagccgtac
     3961 aaatgtacgg ccagcaacgt cggttcgaga tggcgctcga tgacgccaac tacctctgat
     4021 agttgagtcg atacttcggc gatcaccgct tccctcatga tgtttaactt tgttttaggg
     4081 cgactgccct gctgcgtaac atcgttgctg ctccataaca tcaaacatcg acccacggcg
     4141 taacgcgctt gctgcttgga tgcccgaggc atagactgta ccccaaaaaa acatgtcata
     4201 acaagaagcc atgaaaaccg ccggatatat tccgcttcct cgctcactga ctcgctacgc
     4261 tcggtcgttc gactgcggcg agcggaaatg gcttacgaac ggggcggaga tttcctggaa
     4321 gatgccagga agatacttaa cagggaagtg agagggccgc ggcaaagccg tttttccata
     4381 ggctccgccc ccctgacaag catcacgaaa tctgacgctc aaatcagtgg tggcgaaacc
     4441 cgacaggact ataaagatac caggcgtttc cccctggcgg ctccctcgtg cgctctcctg
     4501 ttcctgcctt tcggtttacc ggtgtcattc cgctgttatg gccgcgtttg tctcattcca
     4561 cgcctgacac tcagttccgg gtaggcagtt cgctccaagc tggactgtat gcacgaaccc
     4621 cccgttcagt ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggaa
     4681 agacatgcaa aagcaccact ggcagcagcc actggtaatt gatttagagg agttagtctt
     4741 gaagtcatgc gccggttaag gctaaactga aaggacaagt tttggtgact gcgctcctcc
     4801 aagccagtta cctcggttca aagagttggt agctcagaga accttcgaaa aaccgccctg
     4861 caaggcggtt ttttcgtttt cagagcaaga gattacgcgc agaccaaaac gatctcaaga
     4921 agatcatctt attaatcaga taaaatattt gctcatgagc ccgaagtggc gagcccgatc
     4981 ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat
     5041 gccggccacg atgcgtccgg cgtagaggat ctgctcatgt ttgacagctt atcgagctc
//
