LOCUS       sequence-124129-         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Alpha Actinin, Excitation: 505, Emission: 515.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Lam AJ, St-Pierre F, Gong Y, Marshall JD, Cranfill PJ, Baird MA, 
            McKeown MR, Wiedenmann J, Davidson MW, Schnitzer MJ, Tsien RY, Lin 
            MZ
  TITLE     Improving FRET dynamic range with bright green and red fluorescent 
            proteins.
  JOURNAL   Nat Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171.
  PUBMED    22961245
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171."
COMMENT     Sequence Label: Clover-Alpha-Actinin-19
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(226..247)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     575..594
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt
       61 tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga ttatgatcag ttatctagat
      121 ccggtggatc ccgggcccgc ggtaccgtcg actgcagaat tcttacagct cgtcctttcc
      181 ggacttgtac agctcgtcca tgccgagagt gatcccggcg gcggtcacga actccagcag
      241 gaccatgtga tcgcgcttct cgttggggtc tttgctcagc ttggactggg tgctcaggta
      301 gtggttgtcg ggcagcagca cggggccgtc gccgatgggg gtgttctgct ggtagtggtc
      361 ggcgagctgc acgctgccgt cctcgatgtt gcagttgagg ccgaagttgg ccttgatgcc
      421 gttcttctgc ttgtcggcgg tgatatagac gttgccgtgg atggcgttgt actccagctt
      481 gtgccccagg atgttgccgt cctccttgaa gtcgatgccc ttcagctcga tgcggttcac
      541 cagggtgtcg ccctcgaact tcacctcggc gcgggtcttg tagttgccgt cgtccttgaa
      601 gaagatggtg cgctcctgga cgtagccttc gggcatggcg gacttgaaga agtcgtgctg
      661 cttcatgtgg tcggggtagc gggcgaagca ctgcacgccc cagctcaggg tggtcacgag
      721 ggtgggccag ggcacgggca gcttgccggt ggtgcagatg aacttcaggg tcagcttgcc
      781 gtaggtggca tcgccctcgc
//
