LOCUS       sequence-124136-         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPaint universal donor for TagRFP fusion tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Schmid-Burgk JL, Honing K, Ebert TS, Hornung V
  TITLE     CRISPaint allows modular base-specific gene tagging using a 
            ligase-4-dependent mechanism.
  JOURNAL   Nat Commun. 2016 Jul 28;7:12338. doi: 10.1038/ncomms12338.
  PUBMED    27465542
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-28"; volume: "7:12338. doi"; pages: " 
            10.1038/ncomms12338"
COMMENT     Sequence Label: pCRISPaint-TagRFP
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             36..749
                     /codon_start=1
                     /product="monomeric derivative of red fluorescent protein 
                     from Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /label=TagRFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     primer_bind     complement(768..785)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 aaagcggggg gtctggtggc agtggagggg gatccatggt gtctaagggc gaagagctga
       61 ttaaggagaa catgcacatg aagctgtaca tggagggcac cgtgaacaac caccacttca
      121 agtgcacatc cgagggcgaa ggcaagccct acgagggcac ccagaccatg agaatcaagg
      181 tggtcgaggg cggccctctc cccttcgcct tcgacatcct ggctaccagc ttcatgtacg
      241 gcagcagaac cttcatcaac cacacccagg gcatccccga cttctttaag cagtccttcc
      301 ctgagggctt cacatgggag agagtcacca catacgaaga cgggggcgtg ctgaccgcta
      361 cccaggacac cagcctccag gacggctgcc tcatctacaa cgtcaagatc agaggggtga
      421 acttcccatc caacggccct gtgatgcaga agaaaacact cggctgggag gccaacaccg
      481 agatgctgta ccccgctgac ggcggcctgg aaggcagaag cgacatggcc ctgaagctcg
      541 tgggcggggg ccacctgatc tgcaacttca agaccacata cagatccaag aaacccgcta
      601 agaacctcaa gatgcccggc gtctactatg tggaccacag actggaaaga atcaaggagg
      661 ccgacaaaga gacctacgtc gagcagcacg aggtggctgt ggccagatac tgcgacctcc
      721 ctagcaaact ggggcacaaa cttaattgag cggccgccgc tgatcagcct cgactgtgcc
      781 ttctagttgc cagccatctg
//
