LOCUS       sequence-124138-         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian Expression of Venus-iLID-CAAX.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Guntas G, Hallett RA, Zimmerman SP, Williams T, Yumerefendi H, Bear 
            JE, Kuhlman B
  TITLE     Engineering an improved light-induced dimer (iLID) for controlling 
            the localization and activity of signaling proteins.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Jan 6;112(1):112-7. doi: 
            10.1073/pnas.1417910112. Epub 2014 Dec 22.
  PUBMED    25535392
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1417910112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-01-6- 6"; volume: "112"; issue: "1"; pages: "112-7"
COMMENT     Sequence Label: pLL7.0: Venus-iLID-CAAX (from KRas4B)
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    109..142
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(233..670)
                     /codon_start=1
                     /product="improved light-inducible dimer when used with 
                     SspB nano or SspB micro (Guntas et al., 2015)"
                     /label=iLID
                     /translation="LATTLERIEKNFVITDPRLPDNPIIFASDSFLQLTEYSREEILGR
                     NCRFLQGPETDRATVRKIRDAIDNQTEVTVQLINYTKSGKKFWNVFHLQPMRDYKGDVQ
                     YFIGVQLDGTERLHGAAEREAVCLIKKTAFQIAEAANDENYF"
     primer_bind     complement(734..755)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tagttaagaa taccagtcaa tctttcacaa attttgtaat ccagaggttg attatcgata
       61 agcttgatat cgaattgtac ctagtggaac cggaaccctt aaacatgtat aacttcgtat
      121 aatgtatgct atacgaagtt attaggtccc tcgacgaatt gcctgcaggc tacataatta
      181 cacactttgt ctttgacttc tttttcttct ttttgctccc actcccgctt ccaaagtaat
      241 tttcgtcgtt cgctgcctca gcaatctgaa aggctgtctt tttgatcagg cagaccgcct
      301 cacgctcagc ggcgccatga agacgttcgg tgccatccag ctgcacgcca atgaagtact
      361 ggacgtcgcc cttataatcg cgcatcggct gcaggtggaa cacgttccag aacttcttgc
      421 cgctcttggt ataattgatc agctgaacgg tcacttcagt ctggttgtca atagcatctc
      481 tgattttccg aacagtggca cggtctgtct cgggtccctg cagaaaacgg caattgcgcc
      541 ccaggatctc ttcccgagaa tattctgtca gctgcaggaa ggaatcgctc gcaaaaatga
      601 ttgggttgtc aggcagtctc ggatcagtaa tcacgaaatt tttctcgatc cgttccagtg
      661 tggttgccag aaactcccca ctaccactac ccttgtacag ctcgtccatg ccgagagtga
      721 tcccggcggc ggtcacgaac tccagcagga ccatgtgatc gcgcttctcg ttggggtctt
      781 tgctcagttt ggactggtag ctcaggtagt ggttgtcggg cagcagcacg gggccgtcgc
      841 cgatgggggt
//
