LOCUS       sequence-124146-         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tet-inducible RRNA promoter driven plasmid for the generation of 
            stem loop RNAi transcripts from a random RRNA spacer locus in T. 
            brucei..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  TITLE     Horn Lab plasmid (unpublished)
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pRP-iSL(MCS1+2)
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(175..196)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     387..406
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tcaatacaac acacaatagg ataataatga taaagttaaa aaagtatata tagtaataga
       61 aatatatctt atataggaaa gattaagcag taaaagtagc gcttacggcg tacaccctat
      121 caatgataga gtggtacccg ggcttaagga tccgagatag ggttgagtgt tgttccagtt
      181 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc
      241 tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg
      301 tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga
      361 aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg
      421 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg
      481 ctacagggcg cgtccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc
      541 gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt
      601 gggtaacgcc agggttttct agaagcttct cgagggcccc tgcaggaatt cgatgcgagg
      661 cgaatcgctc agtagtgtta gtgcaggcat tgtttctctt ttccatttcg gctcgctctc
      721 tgtgccagaa aggcttttgg agtcttggtt actcgtggga ctagggcaag cgtcgcttcc
      781 ctaataattg ttgctaacgt ttcaccgtgc gtgtgtggat atcttccaag ttgtcatttt
      841 ctctacccct
//
