LOCUS       sequence-124165-         871 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses 3XturboGFP-NLS-PEST under MERVL_LTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Ishiuchi T, Enriquez-Gasca R, Mizutani E, Boskovic A, 
            Ziegler-Birling C, Rodriguez-Terrones D, Wakayama T, Vaquerizas JM, 
            Torres-Padilla ME
  TITLE     Early embryonic-like cells are induced by downregulating 
            replication-dependent chromatin assembly.
  JOURNAL   Nat Struct Mol Biol. 2015 Aug 3. doi: 10.1038/nsmb.3066.
  PUBMED    26237512
REFERENCE   2  (bases 1 to 871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Struct 
            Mol Biol. 2015 Aug 3. doi: 10.1038/nsmb.3066."
COMMENT     Sequence Label: 2C-3XtbGFP-PEST
FEATURES             Location/Qualifiers
     source          1..871
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(149..169)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(173..193)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(197..217)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tacttgtaca ctacacattg atcctagcag aagcacaggc tgcagggtga cggtccatcc
       61 cgctctcctg ggcacaagac atgggcagcg tgccatcatc ctgctcctcc acctccggcg
      121 ggaagccatg gctagatccg gtggatccta cctttctctt cttttttgga tctacctttc
      181 tcttcttttt tggatctacc tttctcttct tttttggatc agctcgagat ctgagttctt
      241 caccggcatc tgcatccggg gtcttgaagg cgtgctggta ctccacgatg cccagctcgg
      301 tgttgctgtg atcctcctcc acgcggcgga aggcgaacat ggggcccccg ttctgcagga
      361 tgctggggtg gatggcgctc ttgaagtgca tgtggctgtc caccacggag ctgtagtagc
      421 cgccgtcgcg caggctgaag gtgcgggtga agctgccatc cagatcgtta tcgcccatgg
      481 ggtgcaggtg ctccacggtg gcgttgctgc ggatgatctt gtcggtgaag atcacgctgt
      541 cctcggggaa gccggtgccc atcaccttga agtcgccgat cacgcggccg gcctcgtagc
      601 ggtagctgaa gctcacgtgc agcacgccgc cgtcctcgta cttctcgatg cgggtgttgg
      661 tgtagccgcc gttgttgatg gcgtgcagga aggggttctc gtagccgctg gggtaggtgc
      721 cgaagtggta gaagccgtag cccatcacgt ggctcagcag gtaggggctg aaggtcaggg
      781 cgcctttggt gctcttcatc ttgttggtca tgcggccctg ctcgggggtg ccctctccgc
      841 cgcccaccag ctcgaactcc acgccgttca g
//
