LOCUS       sequence-124194-        1123 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Improved version for complementation of yeast auxotrophic marker 
            TRP1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1123)
  AUTHORS   Li D, Song JZ, Shan MH, Li SP, Liu W, Li H, Zhu J, Wang Y, Lin J, 
            Xie Z
  TITLE     A fluorescent tool set for yeast Atg proteins.
  JOURNAL   Autophagy. 2015;11(6):954-60. doi: 10.1080/15548627.2015.1040971.
  PUBMED    25998947
REFERENCE   2  (bases 1 to 1123)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Autophagy."; date: "2015"; volume: "11(6):954-60. doi"; pages: " 
            10.1080/15548627.2015.1040971"
COMMENT     Sequence Label: LD-BS-Trp1
FEATURES             Location/Qualifiers
     source          1..1123
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     4..24
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6..24
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        760..1037
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
ORIGIN
        1 cgcgcaatta accctcacta aagggaacaa aagctggtac tattagctga attgccactg
       61 ctatcgttgt tagtggcgtt agtgcttgca ttcaaagaca tggagggcgt tattacgccg
      121 gagctcctcg acagcagatc tgatgactgg tcaatatatt tttgcattga ggctctgttt
      181 ggaattatat tttgagatga cccatctaat gtactggtat caccagattt catgtcgttt
      241 tttaaagcgg ctgcttgagt cttagcaata gcgtcaccat ctggtgaatc ctttgaagga
      301 accactgacg aaggtttgga cagtgacgaa gaggatcttt cctgctttga attagtcgcg
      361 ctgggagcag atgacgagtt ggtggagctg ggggcaggat tgctggccgt cgtgggtcct
      421 gaatgggtcc ttggctggtc catctctatt ctgaaaacgg aagaggagta gggaatatta
      481 ctggctgaaa ataagtcttg aatgaacgta tacgcgtata tttctaccaa tctctcaaca
      541 ctgagtaatg gtagttataa gaaagagacc gagttaggga cagttagagg cggtggagat
      601 attccttatg gcatgtctgg cgatgataaa acttttcaaa cggcagcccc gatctaaaag
      661 agctgacagg gaaatggtca gaaaaagaaa cgtgcacccg cccgtctgga cgcgccgctc
      721 acccgcacgg cagagaccaa tcagtaaaaa tcaacggtta acgacattac tatatatata
      781 atataggaag catttaatag aacagcatcg taatatatgt gtactttgca gttatgacgc
      841 cagatggcag tagtggaaga tattctttat tgaaaaatag cttgtcacct tacgtacaat
      901 cttgatccgg agcttttctt tttttgccga ttaagaattc ggtcgaaaaa agaaaaggag
      961 agggccaaga gggagggcat tggtgactat tgagcacgtg agtatacgtg attaagcaca
     1021 caaaggcagc ttggagtatg ctcgttaaag tgtgtggttt gcaaaccgtt gaagctgcaa
     1081 agactgctgt ggatgatggt gctgattact taggtatcat ttg
//
