LOCUS       sequence-124208-       10434 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Human CDK19 W105M Mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10434)
  AUTHORS   Pelish HE, Liau BB, Nitulescu II, Tangpeerachaikul A, Poss ZC, Da 
            Silva DH, Caruso BT, Arefolov A, Fadeyi O, Christie AL, Du K, Banka 
            D, Schneider EV, Jestel A, Zou G, Si C, Ebmeier CC, Bronson RT, 
            Krivtsov AV, Myers AG, Kohl NE, Kung AL, Armstrong SA, Lemieux ME, 
            Taatjes DJ, Shair MD
  TITLE     Mediator kinase inhibition further activates 
            super-enhancer-associated genes in AML.
  JOURNAL   Nature. 2015 Sep 28. doi: 10.1038/nature14904.
  PUBMED    26416749
REFERENCE   2  (bases 1 to 10434)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Sep 28. doi: 10.1038/nature14904."
COMMENT     Sequence Label: pLVX-Flag-CDK19-W105M-IRES-ZsGreen
FEATURES             Location/Qualifiers
     source          1..10434
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2337..3519
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2568..3510
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3467..3487
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3559..3582
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    5120..5692
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5143..5694
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5287..5304)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             5694..6389
                     /codon_start=1
                     /product="Zoanthus green fluorescent protein"
                     /label=ZsGreen1
                     /note="mammalian codon-optimized"
                     /translation="MAQSKHGLTKEMTMKYRMEGCVDGHKFVITGEGIGYPFKGKQAIN
                     LCVVEGGPLPFAEDILSAAFMYGNRVFTEYPQDIVDYFKNSCPAGYTWDRSFLFEDGAV
                     CICNADITVSVEENCMYHESKFYGVNFPADGPVMKKMTDNWEPSCEKIIPVPKQGILKG
                     DVSMYLLLKDGGRLRCQFDTVYKAKSVPRKMPDWHFIQHKLTREDRSDAKNQKWHLTEH
                     AIASGSALP"
     misc_feature    6403..6991
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6456..6476)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6874..6885)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7198..7831
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7959..7975)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7959..7975)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7972..7994)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7983..7999
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8007..8013,8014..8031,8032..8037))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8052..8073
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8190..8207)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8361..8949)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8441..8460)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9120..9911,9912..9980))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9743..9762
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9981..10085)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    10133..10267
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10170..10189)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10224..10243
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgagtaa ttcatacaaa aggactcgcc cctgccttgg
     2221 ggaatcccag ggaccgtcgt taaactccca ctaacgtaga acccagagat cgctgcgttc
     2281 ccgccccctc acccgcccgc tctcgtcatc actgaggtgg agaagagcat gcgtgaggct
     2341 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag
     2401 gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg
     2461 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag
     2521 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt
     2581 gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt
     2641 ccacgcccct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
     2701 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
     2761 cttgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
     2821 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
     2881 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
     2941 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
     3001 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
     3061 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
     3121 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
     3181 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
     3241 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
     3301 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     3361 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     3421 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     3481 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gatctatttc cggtgaattc
     3541 ctcgagacta gtatgccaga ctacaaggac gatgatgaca agctcgagat ggattatgat
     3601 ttcaaggcga agctggcggc ggagcgggag cgggtggagg atttgtttga gtacgaaggg
     3661 tgcaaagtgg gacgcggcac ctacggtcac gtctacaagg cgaggcggaa agatggaaaa
     3721 gatgaaaagg aatatgcatt gaagcaaatt gaaggcacag gaatatccat gtcggcttgt
     3781 agagagattg cacttttgcg agaattgaag caccctaatg tgattgcatt gcagaaggtg
     3841 ttcctttctc acagtgacag gaaggtatgg ctgctgtttg attatgcaga gcatgacttg
     3901 atgcatatta ttaagtttca ccgtgcatca aaagcaaata aaaagcccat gcagttgcca
     3961 agatctatgg ttaaatcctt actttaccag attcttgatg gtatccatta cctccatgca
     4021 aattgggtgc ttcacagaga cttgaaacca gcaaatatcc tagtaatggg agaaggtcct
     4081 gagaggggga gagtcaaaat agctgacatg ggttttgcca gattattcaa ttctcctcta
     4141 aagccactag cagatttgga tccagtagtt gtgacatttt ggtatcgggc tccagaactt
     4201 ttgcttggtg caaggcatta tacaaaggcc attgatatat gggcaatagg ttgtatattt
     4261 gctgaattgt tgacttcgga acctattttt cactgtcgtc aggaagatat aaaaacaagc
     4321 aatccctttc atcatgatca actggatcgg atatttagtg tcatggggtt tcctgcagat
     4381 aaagactggg aagatattag aaagatgcca gaatatccca cacttcaaaa agactttaga
     4441 agaacaacgt atgccaacag tagcctcata aagtacatgg agaaacacaa ggtcaagcct
     4501 gacagcaaag tgttcctctt gcttcagaaa ctcctgacca tggatccaac caagagaatt
     4561 acctcggagc aagctctgca ggatccctat tttcaggagg accctttgcc aacattagat
     4621 gtatttgccg gctgccagat tccatacccc aaacgagaat tccttaatga agatgatcct
     4681 gaagaaaaag gtgacaagaa tcagcaacag cagcagaacc agcatcagca gcccacagcc
     4741 cctccacagc aggcagcagc ccctccacag gcgcccccac cacagcagaa cagcacccag
     4801 accaacggga ccgcaggtgg ggctggggcc ggggtcgggg gcaccggagc agggttgcag
     4861 cacagccagg actccagcct gaaccaggtg cctccaaaca agaagccacg gctagggcct
     4921 tcaggcgcaa actcaggtgg acctgtgatg ccctcggatt atcagcactc cagttctcgc
     4981 ctgaattacc aaagcagcgt tcagggatcc tctcagtccc agagcacact tggctactct
     5041 tcctcgtctc agcagagctc acagtaccac ccatctcacc aggcccaccg gtactgatct
     5101 agagcggccg cggatcccgc ccctctccct cccccccccc taacgttact ggccgaagcc
     5161 gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt
     5221 ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc
     5281 tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc
     5341 tggaagcttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc
     5401 cacctggcga caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg
     5461 cggcacaacc ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctca
     5521 cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat
     5581 ctgatctggg gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaacgtct
     5641 aggccccccg aaccacgggg acgtggtttt cctttgaaaa acacgatgat aatatggccc
     5701 agtccaagca cggcctgacc aaggagatga ccatgaagta ccgcatggag ggctgcgtgg
     5761 acggccacaa gttcgtgatc accggcgagg gcatcggcta ccccttcaag ggcaagcagg
     5821 ccatcaacct gtgcgtggtg gagggcggcc ccttgccctt cgccgaggac atcttgtccg
     5881 ccgccttcat gtacggcaac cgcgtgttca ccgagtaccc ccaggacatc gtcgactact
     5941 tcaagaactc ctgccccgcc ggctacacct gggaccgctc cttcctgttc gaggacggcg
     6001 ccgtgtgcat ctgcaacgcc gacatcaccg tgagcgtgga ggagaactgc atgtaccacg
     6061 agtccaagtt ctacggcgtg aacttccccg ccgacggccc cgtgatgaag aagatgaccg
     6121 acaactggga gccctcctgc gagaagatca tccccgtgcc caagcagggc atcttgaagg
     6181 gcgacgtgag catgtacctg ctgctgaagg acggtggccg cttgcgctgc cagttcgaca
     6241 ccgtgtacaa ggccaagtcc gtgccccgca agatgcccga ctggcacttc atccagcaca
     6301 agctgacccg cgaggaccgc agcgacgcca agaaccagaa gtggcacctg accgagcacg
     6361 ccatcgcctc cggctccgcc ttgccctgaa cgcgtctgga acaatcaacc tctggattac
     6421 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     6481 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     6541 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     6601 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     6661 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     6721 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     6781 gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg
     6841 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     6901 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     6961 agtcggatct ccctttgggc cgcctccccg cctggaatta attctgcagt cgagacctag
     7021 aaaaacatgg agcaatcaca agtagcaata cagcagctac caatgctgat tgtgcctggc
     7081 tagaagcaca agaggaggag gaggtgggtt ttccagtcac acctcaggta cctttaagac
     7141 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag aggggactgg
     7201 aagggctaat tcactcccaa cgaagacaag atatccttga tctgtggatc taccacacac
     7261 aaggctactt ccctgattag cagaactaca caccagggcc aggggtcaga tatccactga
     7321 cctttggatg gtgctacaag ctagtaccag ttgagccaga taaggtagaa gaggccaata
     7381 aaggagagaa caccagcttg ttacaccctg tgagcctgca tgggatggat gacccggaga
     7441 gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg gcccgagagc
     7501 tgcatccgga gtacttcaag aactgctgat atcgagcttg ctacaaggga ctttccgctg
     7561 gggactttcc agggaggcgt ggcctgggcg ggactgggga gtggcgagcc ctcagatcct
     7621 gcatataagc agctgctttt tgcctgtact gggtctctct ggttagacca gatctgagcc
     7681 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     7741 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     7801 cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag
     7861 tatttataac ttgcaaagaa atgaatatca gagagtgaga ggccttgaca ttgctagcgt
     7921 ttaccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga
     7981 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     8041 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     8101 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     8161 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     8221 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     8281 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     8341 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     8401 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     8461 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     8521 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     8581 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     8641 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     8701 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     8761 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     8821 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     8881 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     8941 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     9001 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     9061 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     9121 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     9181 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     9241 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     9301 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     9361 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     9421 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     9481 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     9541 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     9601 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     9661 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     9721 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     9781 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     9841 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     9901 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     9961 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
    10021 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
    10081 ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tcaacttgtt
    10141 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
    10201 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
    10261 ctggatcaac tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc
    10321 tattaccact gccaattacc tgtggtttca tttactctaa acctgtgatt cctctgaatt
    10381 attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag tagt
//
