LOCUS       sequence-124218-         478 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a toehold mutant of the circRAJ31 riboregulator encased in
            a permuted intron exon ribozyme, which circularises the 
            riboregulator. This mutant does not activate cisRAJ31..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 478)
  AUTHORS   Rostain W, Shen S, Cordero T, Rodrigo G, Jaramillo A
  TITLE     Engineering a Circular Riboregulator in Escherichia coli.
  JOURNAL   Biodes Res. 2020 Sep 12;2020:1916789. doi: 10.34133/2020/1916789. 
            eCollection 2020.
  PUBMED    37849901
REFERENCE   2  (bases 1 to 478)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biodes 
            Res."; date: "2020-09-12"; volume: "2020:1916789. doi"; pages: " 
            10.34133/2020/1916789. eCollection 2020"
COMMENT     Sequence Label: circRAJ31-Toehold_mutant
FEATURES             Location/Qualifiers
     source          1..478
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    7..25
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    32..50
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 gaattctccc tatcagtgat agagattgac atccctatca gtgatagaga tactgagcac
       61 tctagaggtt ctacataaat gcctaacgac tatccctttg gggagtaggg tcaagtgact
      121 cgaaacgata gacaacttgc tttaacaagt tggagatata gtctgctctg catggtgaca
      181 tgcagctgga tataattccg gggtaagatt aacgacctta tctgaacata atgctaccgt
      241 ttaatattcc taggggatca atattaagct ttaccgacac cccatccgca attaagtccc
      301 tatccgctgt cactgtattg gtctgctagc atgttttctt gggttaattg aggcctgagt
      361 ataaggtgac ttatacttgt aatctatcta aacggggaac ctctctagta gacaatcccg
      421 tgctaaattg taggactcaa ttgatcctta gcgaaagcta aggatttttt ttctgcag
//
