LOCUS       sequence-124219-        2271 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses taRAJ31min, a linear riboregulator..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Rostain W, Shen S, Cordero T, Rodrigo G, Jaramillo A
  TITLE     Engineering a Circular Riboregulator in Escherichia coli.
  JOURNAL   Biodes Res. 2020 Sep 12;2020:1916789. doi: 10.34133/2020/1916789. 
            eCollection 2020.
  PUBMED    37849901
REFERENCE   2  (bases 1 to 2271)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biodes 
            Res."; date: "2020-09-12"; volume: "2020:1916789. doi"; pages: " 
            10.34133/2020/1916789. eCollection 2020"
COMMENT     Sequence Label: taRAJ31min
FEATURES             Location/Qualifiers
     source          1..2271
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     5..22
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6..22
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    197..215
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    222..240
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(479..495)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(479..495)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      543..574
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        575..677
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             join(678..746,747..1538)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESLRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLATIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGSLLRSA
                     LPAGWFIADKSGAGERGTRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(896..915)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      1610..1637
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1649..2236)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1729..1748)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cccgtgtaaa acgacggcca gtttatctag tcagcttgat tctagctgat cgtggaccgg
       61 aaggtgagcc agtgagttga ttgcagtcca gttacgctgg agtctgaggc tcgtcctgaa
      121 tgatatgcga ccgccggagg gttgcgtttg agacgggcga cagatcgaca ctgctcgatc
      181 cgctcgcacc gaattctccc tatcagtgat agagattgac atccctatca gtgatagaga
      241 tactgagcac ggatcaatat taagctttac cgtcgtctca tccgcaatta agtccctatc
      301 cgctgtcact gtattggtct atccttagcg aaagctaagg attttttttc tgcagggatc
      361 gacgagagca gcgcgactgg atcagttctg gacgagcgag ctgtcgtccg acccgtgatc
      421 ttacggcatt atacgtatga tcggtccacg atcagctaga ttatctagtc agcttgatgt
      481 catagctgtt tcctgaggct caatactgac catttaaatc atacctgacc tccatagcag
      541 aaagtcaaaa gcctccgacc ggaggctttt gacttgatcg gcacgtaaga ggttccaact
      601 ttcaccataa tgaaataaga tcactaccgg gcgtattttt tgagttatcg agattttcag
      661 gagctaagga agctaaaatg agtattcaac atttccgtgt cgcccttatt cccttttttg
      721 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
      781 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
      841 ttgagagttt acgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
      901 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
      961 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctc acggatggca
     1021 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     1081 tacttctggc aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     1141 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     1201 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     1261 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     1321 caggatcact tctgcgctcg gccctcccgg ctggctggtt tattgctgat aaatctggag
     1381 ccggtgagcg tgggactcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     1441 gcatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     1501 tcgctgagat aggtgcctca ctgattaagc attggtaatg agggcccaaa tgtaatcacc
     1561 tggctcacct tcgggtgggc ctttcttgag gacctaaatg taatcacctg gctcaccttc
     1621 gggtgggcct ttctgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     1681 acaaaaatcg atgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     1741 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     1801 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     1861 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     1921 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     1981 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     2041 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     2101 gtatctgcgc tctgctgaag ccagttacct cggaaaaaga gttggtagct cttgatccgg
     2161 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     2221 aaaaaaagga tctcaagaag atcctttgat tttctaccga agaaaggccc a
//
