LOCUS       sequence-124223-         956 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  The first of the two RNA lassos required for the RNA chain 
            construction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 956)
  AUTHORS   Hassall J, MacDonald P, Cordero T, Rostain W, Jaramillo A
  TITLE     Design and Characterization of Topological Small RNAs.
  JOURNAL   Methods Mol Biol. 2015;1316:149-67. doi: 
            10.1007/978-1-4939-2730-2_13.
  PUBMED    25967060
REFERENCE   2  (bases 1 to 956)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2015"; volume: "1316:149-67. doi"; pages: " 
            10.1007/978-1-4939-2730-2_13"
COMMENT     Sequence Label: Lasso 1
FEATURES             Location/Qualifiers
     source          1..956
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(21..64)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     82..101
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        82..100
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      296..343
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      359..417
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(441..458)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(692..711)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tnggcgtatc acgaggcaga atttcagata aaaaaaatcc ttagctttcg ctaaggntga
       61 tttctggaat tcgaagacgc ttaatacgac tcactatagg gaggctgtcc tcgtccgtat
      121 gacgagagaa gcctaccaga gaaacacacg acgtaagtcg tggtacatta cctggtacag
      181 ttctcttcaa gggacaaggc taacaacaac taacaacata caatcaacat aatttgccat
      241 ctgggtaaca acaacataca atcaacaatc aaccaaggct gtcctcgtcg atatcctagc
      301 ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgagcctct tcctgcagtc
      361 cggcaaaaaa acggcaaggt gtcaccaccc tgcccttttt ctttaaaacc gaaaagatta
      421 cttcgcgtta tgcaggcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
      481 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
      541 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
      601 ttgctggcgt ttttccacag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
      661 agtcanaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
      721 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
      781 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggngtag
      841 gtcgttcgct ccnagctggg ctgtgtgcac gaaccccccg ttcagcccga cgctgcgcct
      901 ttatccggta actatcgtct tgagtccnac ccggtaanac acgacttatc gccact
//
