LOCUS       sequence-124225-         451 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses RAJ31 riboregulator encased in a permuted intron exon 
            ribozyme, which circularises the riboregulator. This mutant is 
            deficient in circularisation. Under control of a T7 promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Rostain W, Shen S, Cordero T, Rodrigo G, Jaramillo A
  TITLE     Engineering a Circular Riboregulator in Escherichia coli.
  JOURNAL   Biodes Res. 2020 Sep 12;2020:1916789. doi: 10.34133/2020/1916789. 
            eCollection 2020.
  PUBMED    37849901
REFERENCE   2  (bases 1 to 451)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biodes 
            Res."; date: "2020-09-12"; volume: "2020:1916789. doi"; pages: " 
            10.34133/2020/1916789. eCollection 2020"
COMMENT     Sequence Label: pT7_circRAJ31_C873U
FEATURES             Location/Qualifiers
     source          1..451
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      404..451
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 taatacgact cactataggg tctagaggtt ctacataaat gcctaacgat tatccctttg
       61 gggagtaggg tcaagtgact cgaaacgata gacaacttgc tttaacaagt tggagatata
      121 gtctgctctg catggtgaca tgcagctgga tataattccg gggtaagatt aacgacctta
      181 tctgaacata atgctaccgt ttaatattcc taggggatca atattaagct ttaccgtcgt
      241 ctcatccgca attaagtccc tatccgctgt cactgtattg gtctgctagc atgttttctt
      301 gggttaattg aggcctgagt ataaggtgac ttatacttgt aatctatcta aacggggaac
      361 ctctctagta gacaatcccg tgctaaattg taggactcaa ttgctagcat aaccccttgg
      421 ggcctctaaa cgggtcttga ggggtttttt g
//
