LOCUS       sequence-124228-         872 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses cisRAJ31-GFP-LAA, a cis-repressed mRNA regulated by 
            RAJ31..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Rostain W, Shen S, Cordero T, Rodrigo G, Jaramillo A
  TITLE     Engineering a Circular Riboregulator in Escherichia coli.
  JOURNAL   Biodes Res. 2020 Sep 12;2020:1916789. doi: 10.34133/2020/1916789. 
            eCollection 2020.
  PUBMED    37849901
REFERENCE   2  (bases 1 to 872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biodes 
            Res."; date: "2020-09-12"; volume: "2020:1916789. doi"; pages: " 
            10.34133/2020/1916789. eCollection 2020"
COMMENT     Sequence Label: cisRAJ31-GFP-LAA
FEATURES             Location/Qualifiers
     source          1..872
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    27..43
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             90..101
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             117..827
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="RKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
ORIGIN
        1 ataaatgtga gcggataaca ttgacattgt gagcggataa caagatactg agcacagtgg
       61 atatgcttta cctcttggtg agacgacata aagaggagaa acataatatg actagccgta
      121 aaggcgaaga gctgttcact ggtgtcgtcc ctattctggt ggaactggat ggtgatgtca
      181 acggtcataa gttttccgtg cgtggcgagg gtgaaggtga cgcaactaat ggtaaactga
      241 cgctgaagtt catctgtact actggtaaac tgccggtacc ttggccgact ctggtaacga
      301 cgctgactta tggtgttcag tgctttgctc gttatccgga ccatatgaag cagcatgact
      361 tcttcaagtc cgccatgccg gaaggctatg tgcaggaacg cacgatttcc tttaaggatg
      421 acggcacgta caaaacgcgt gcggaagtga aatttgaagg cgataccctg gtaaaccgca
      481 ttgagctgaa aggcattgac tttaaagaag acggcaatat cctgggccat aagctggaat
      541 acaattttaa cagccacaat gtttacatca ccgccgataa acaaaaaaat ggcattaaag
      601 cgaattttaa aattcgccac aacgtggagg atggcagcgt gcagctggct gatcactacc
      661 agcaaaacac tccaatcggt gatggtcctg ttctgctgcc agacaatcac tatctgagca
      721 cgcaaagcgt tctgtctaaa gatccgaacg agaaacgcga tcatatggtt ctgctggagt
      781 tcgtaaccgc agcgggcatc acgcatggta tggatgaact gtacaaagct agcgcagcga
      841 acgacgaaaa ttacgccctt gcagcgtaat aa
//
