LOCUS       sequence-124240-        1156 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  The second of the two RNA lassos required for the RNA chain 
            construction..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1156)
  AUTHORS   Hassall J, MacDonald P, Cordero T, Rostain W, Jaramillo A
  TITLE     Design and Characterization of Topological Small RNAs.
  JOURNAL   Methods Mol Biol. 2015;1316:149-67. doi: 
            10.1007/978-1-4939-2730-2_13.
  PUBMED    25967060
REFERENCE   2  (bases 1 to 1156)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2015"; volume: "1316:149-67. doi"; pages: " 
            10.1007/978-1-4939-2730-2_13"
COMMENT     Sequence Label: Lasso 2
FEATURES             Location/Qualifiers
     source          1..1156
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     13..31
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(35..78)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     96..115
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        96..114
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      310..357
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      373..431
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(455..472)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(706..725)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 anttactata aaaataggcg tatcacgagg cagaatttca gataaaaaaa atccttagct
       61 ttcgctaagg atgatttctg gaattcgaag acgcttaata cgactcacta tagggaggct
      121 gtcctcgtcc gtatgacgag agaagcctac cagagaaaca cacgacgtaa gtcgtggtac
      181 attacctggt acaagccttg tcccttgaag agaacaacta acaactaaca acaacaatca
      241 actattaaac ggtagaccca aacatacaac aacaatcaac aatcaaccaa ggctgtcctc
      301 gtcgatatcc tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgagc
      361 ctcttcctgc agtccggcaa aaaaacggca aggtgtcacc accctgccct ttttctttaa
      421 aaccgaaaag attacttcgc gttatgcagg cttcctcgct cactgactcg ctgcgctcgg
      481 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
      541 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
      601 gtaaaaaggc cgcgttgctg gcgtttttcc acaggctccg cccccctgac gagcatcaca
      661 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
      721 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
      781 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
      841 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
      901 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
      961 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggnggtg
     1021 ctacagagtt cttgaagtgg tggcctaact acggctacnc tagaanaaca gtatttggta
     1081 tctgcgctct gctgaagcca gttaccttcg gaaaannnnt tggtanntct tgatccggna
     1141 ancaaaccac cgctgg
//
