LOCUS       sequence-124246-        1135 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Construct targeting the PIE-target sequence..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1135)
  AUTHORS   Hassall J, MacDonald P, Cordero T, Rostain W, Jaramillo A
  TITLE     Design and Characterization of Topological Small RNAs.
  JOURNAL   Methods Mol Biol. 2015;1316:149-67. doi: 
            10.1007/978-1-4939-2730-2_13.
  PUBMED    25967060
REFERENCE   2  (bases 1 to 1135)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2015"; volume: "1316:149-67. doi"; pages: " 
            10.1007/978-1-4939-2730-2_13"
COMMENT     Sequence Label: AL562-2
FEATURES             Location/Qualifiers
     source          1..1135
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     9..27
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(31..74)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     92..111
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        92..110
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      254..301
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      317..375
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(399..416)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(650..669)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 anctataaaa taggcgtatc acgaggcaga atttcagata aaaaaaatcc ttagctttcg
       61 ctaaggatga tttctggaat tcgaagacgc ttaatacgac tcactatagg gaggctgtcc
      121 tcgtccgtat gacgagagaa gcctaccaga gaaacacacg acgtaagtcg tggtacatta
      181 cctggtacat attaaacggt agacccaaac aacaacaaga acaacaacaa ccaaggctgt
      241 cctcgtctgc gcactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt
      301 gagcctcttc ctgcagtccg gcaaaaaaac ggcaaggtgt caccaccctg ccctttttct
      361 ttaaaaccga aaagattact tcgcgttatg caggcttcct cgctcactga ctcgctgcgc
      421 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
      481 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
      541 aaccgtaaaa aggccgcgtt gctggcgttt ttccacaggc tccgcccccc tgacgagcat
      601 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
      661 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
      721 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
      781 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
      841 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
      901 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
      961 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     1021 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaan gagttggtag ctcttgatcc
     1081 ggcaanaaac caccgctggt agcggnggnt ttttttgttt gcaagcagca gatta
//
