LOCUS       sequence-124258-        8164 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses GFP-labeled mitochondrial outer membrane protein 25.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8164)
  AUTHORS   Katajisto P, Dohla J, Chaffer CL, Pentinmikko N, Marjanovic N, Iqbal
            S, Zoncu R, Chen W, Weinberg RA, Sabatini DM
  TITLE     Stem cells. Asymmetric apportioning of aged mitochondria between 
            daughter cells is required for stemness.
  JOURNAL   Science. 2015 Apr 17;348(6232):340-3. doi: 10.1126/science.1260384. 
            Epub 2015 Apr 2.
  PUBMED    25837514
REFERENCE   2  (bases 1 to 8164)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1260384"; journalName: "Science"; date: 
            "2015-04-17- 17"; volume: "348"; issue: "6232"; pages: "340-3"
COMMENT     Sequence Label: paGFP-Omp25
FEATURES             Location/Qualifiers
     source          1..8164
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        131..334
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     285..305
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      363..372
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(369..371,372..374,375..1085)
                     /codon_start=1
                     /product="photoactivatable GFP variant (Patterson et al., 
                     2002)"
                     /label=PA-GFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(369..371,372..374,375..1085)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(414..435)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(675..694)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1022..1043
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    1247..1364
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1413..1923
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     1842..1860
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             1945..2544
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1945..1964)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2441..2461
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             2672..2905
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2977..3098
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3014..3033)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3068..3087
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3138..3273
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3293..3312)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3294..3312)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3322..3339)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3322..3338)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3331..3353)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3480..3935
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3567..3586)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3777..3798
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3961..4065
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4066..4134,4135..4926)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4284..4303)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5097..5685
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5586..5605
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5839..5856
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5973..5994
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6009..6014,6015..6032,6033..6039)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6047..6063
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6052..6074
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6071..6087
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6071..6087
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6106..6126
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6108..6126
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6154..6380
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6381..6561
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6608..6733
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7226..7459
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7644..7688
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     7986..8006
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
       61 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      121 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
      181 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
      241 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
      301 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta
      361 gcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
      421 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
      481 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
      541 ggcccaccct cgtgaccacc ttttcctacg gcgtgcagtg cttcagccgc taccccgacc
      601 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
      661 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
      721 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
      781 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc
      841 agaagaacgg catcaaggcg aactttaaga tccgccacaa catcgaggac ggcagcgtgc
      901 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
      961 acaaccacta cctgagccac cagtccaaac tgagcaaaga ccccaacgag aagcgcgatc
     1021 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
     1081 acaagacgcg tgggcgaggc gacggagagc cgagtggagt tcctgtagct gtggtgctgc
     1141 tgccagtgtt tgcccttacc ctggtagcag tttgggcctt cgtgagatac cgaaagcagc
     1201 tctgagaatt ctcgacctcg agacaaatgg cagtattcat ccacaatttt aaaagaaaag
     1261 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     1321 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     1381 acagcagaga tccactttgg ccgcggctcg agggggttgg ggttgcgcct tttccaaggc
     1441 agccctgggt ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa acgcagcggc
     1501 gccgaccctg ggtctcgcac attcttcacg tccgttcgca gcgtcacccg gatcttcgcc
     1561 gctacccttg tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg ggaaggttcc
     1621 ttgcggttcg cggcgtgccg gacgtgacaa acggaagccg cacgtctcac tagtaccctc
     1681 gcagacggac agcgccaggg agcaatggca gcgcgccgac cgcgatgggc tgtggccaat
     1741 agcggctgct cagcagggcg cgccgagagc agcggccggg aaggggcggt gcgggaggcg
     1801 gggtgtgggg cggtagtgtg ggccctgttc ctgcccgcgc ggtgttccgc attctgcaag
     1861 cctccggagc gcacgtcggc agtcggctcc ctcgttgacc gaatcaccga cctctctccc
     1921 cagggggatc caccggagct taccatgacc gagtacaagc ccacggtgcg cctcgccacc
     1981 cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc
     2041 acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc
     2101 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg
     2161 gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc
     2221 ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc
     2281 ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc
     2341 gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag
     2401 cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag
     2461 cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc
     2521 atgacccgca agcccggtgc ctgacgcccg ccccacgacc cgcagcgccc gaccgaaagg
     2581 agcgcacgac cccatgcatc ggtaccttta agaccaatga cttacaaggc agctgtagat
     2641 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga
     2701 caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag
     2761 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     2821 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     2881 tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta
     2941 taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat
     3001 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     3061 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc
     3121 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     3181 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
     3241 tccagaagta gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag
     3301 tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
     3361 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
     3421 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga
     3481 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     3541 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     3601 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     3661 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     3721 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     3781 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     3841 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     3901 cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg
     3961 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     4021 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     4081 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     4141 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     4201 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     4261 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     4321 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     4381 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     4441 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     4501 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     4561 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     4621 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     4681 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     4741 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     4801 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     4861 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     4921 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     4981 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     5041 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     5101 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     5161 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     5221 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     5281 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     5341 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     5401 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     5461 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     5521 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     5581 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     5641 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     5701 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     5761 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     5821 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     5881 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     5941 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
     6001 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
     6061 caatttcaca caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac
     6121 taaagggaac aaaagctgga gctgcaagct taatgtagtc ttatgcaata ctcttgtagt
     6181 cttgcaacat ggtaacgatg agttagcaac atgccttaca aggagagaaa aagcaccgtg
     6241 catgccgatt ggtggaagta aggtggtacg atcgtgcctt attaggaagg caacagacgg
     6301 gtctgacatg gattggacga accactgaat tgccgcattg cagagatatt gtatttaagt
     6361 gcctagctcg atacataaac gggtctctct ggttagacca gatctgagcc tgggagctct
     6421 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     6481 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     6541 cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc
     6601 agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg
     6661 gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg
     6721 gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt
     6781 aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct
     6841 agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact
     6901 gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac
     6961 agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt
     7021 agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccgctga
     7081 tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata
     7141 aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc
     7201 agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag
     7261 gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg
     7321 gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc
     7381 aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc
     7441 taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg
     7501 ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga
     7561 cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg
     7621 aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg
     7681 caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa
     7741 tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata
     7801 gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac
     7861 ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc
     7921 gattagtgaa cggatctcga cggtatcgat cacgagacta gcctcgagcg gccgccccct
     7981 tcaccgaggg cctatttccc atgattcctt catatttgca tatacgatac aaggctgtta
     8041 gagagataat tggaattaat ttgactgtaa acacaaagat attagtacaa aatacgtgac
     8101 gtagaaagta ataatttctt gggtagtttg cagttttaaa attatgtttt aaaatggact
     8161 atca
//
