LOCUS       sequence-124259-         926 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Nachury MV, Loktev AV, Zhang Q, Westlake CJ, Peranen J, Merdes A, 
            Slusarski DC, Scheller RH, Bazan JF, Sheffield VC, Jackson PK
  TITLE     A core complex of BBS proteins cooperates with the GTPase Rab8 to 
            promote ciliary membrane biogenesis.
  JOURNAL   Cell. 2007 Jun 15. 129(6):1201-13.
  PUBMED    17574030
REFERENCE   2  (bases 1 to 926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2007 
            Jun 15. 129(6):1201-13."
COMMENT     Sequence Label: pGFP-Rab8A
FEATURES             Location/Qualifiers
     source          1..926
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(103..105,106..108,109..819)
                     /codon_start=1
                     /product="Cycle 3 GFP (Crameri et al., 1996)"
                     /label=Cycle 3 GFP
                     /note="mammalian codon-optimized"
                     /translation="MASKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(136..164)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     759..779
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     protein_bind    841..865
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc tggctagaca ccatggccag caaaggagaa
      121 gaacttttca ctggagttgt cccaattctt gttgaattag atggtgatgt taatgggcac
      181 aaattttctg tcagtggaga gggtgaaggt gatgctacat acggaaagct tacccttaaa
      241 tttatttgca ctactggaaa actacctgtt ccatggccaa cacttgtcac tactttctct
      301 tatggtgttc aatgcttttc ccgttatccg gatcatatga aacggcatga ctttttcaag
      361 agtgccatgc ccgaaggtta tgtacaggaa cgcactatat ctttcaaaga tgacgggaac
      421 tacaagacgc gtgctgaagt caagtttgaa ggtgataccc ttgttaatcg tatcgagtta
      481 aaaggtattg attttaaaga agatggaaac attctcggac acaaactcga gtacaactat
      541 aactcacaca atgtatacat cacggcagac aaacaaaaga atggaatcaa agctaacttc
      601 aaaattcgcc acaacattga agatggatcc gttcaactag cagaccatta tcaacaaaat
      661 actccaattg gcgatggccc tgtcctttta ccagacaacc attacctgtc gacacaatct
      721 gccctttcga aagatcccaa cgaaaagcgt gaccacatgg tccttcttga gtttgtaact
      781 gctgctggga ttacacatgg catggatgag ctctacaaaa gcggttccgg accggatcaa
      841 acaagtttgt acaaaaaagc aggcaccatg gcgaagacct acgattacct gttcaagctg
      901 ctgctgatcg gggactcggg ggtggg
//
