LOCUS       sequence-12430-e       10809 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10809)
  AUTHORS   Semple JI, Garcia-Verdugo R, Lehner B
  TITLE     Rapid selection of transgenic C. elegans using antibiotic 
            resistance.
  JOURNAL   Nat Methods. 2010 Sep;7(9):725-7. doi: 10.1038/nmeth.1495. Epub 2010
            Aug 22.
  PUBMED    20729840
REFERENCE   2  (bases 1 to 10809)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1495"; journalName: "Nat Methods"; date: "2010-09"; 
            volume: "7"; issue: "9"; pages: "725-7"
COMMENT     Sequence Label: pBCN27-R4R3
FEATURES             Location/Qualifiers
     source          1..10809
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(146..734)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(226..245)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(905..1696,1697..1765))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1528..1547
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1766..1870)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1938..1956
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1994..2016)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2116..2135
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4281..4301)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     4880..4899
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5515..5532
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5516..5532
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5540..5663
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(5688..5693,5694..5711,5712..5718)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             5772..6431
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(5838..5857)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             6773..7078
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     6957..6976
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(7119..7242)
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             9308..9319
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 tgtgaaattg ttatccgctg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
       61 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag accaggaacc
      121 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
      181 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
      241 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
      301 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
      361 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
      421 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
      481 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
      541 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
      601 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
      661 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
      721 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
      781 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
      841 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
      901 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
      961 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     1021 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     1081 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     1141 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     1201 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     1261 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     1321 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     1381 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     1441 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     1501 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     1561 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     1621 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     1681 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     1741 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     1801 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     1861 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
     1921 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg
     1981 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
     2041 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
     2101 gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg
     2161 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc
     2221 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
     2281 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
     2341 gtcacgtgtg tttttgaaca cggcgatatg tatctgtaga tcatccccaa ttttcaacaa
     2401 tgaatctaaa tactgaactt tttgtgtaat ctttagtgca gaaaaatgac caaaatcagc
     2461 gattttccga ttatacccaa aatatctcag ctcaatgagc acctataaaa atgtaaaaat
     2521 aaagaaagta gtcgaaccat attttgtaaa ggctaaaaaa ctgaataaat ttttatccat
     2581 ccagcgcggc gttacaatcc gtggcccatg tcacgcacgg ctttcaaaga aaaaaaaaca
     2641 gtctgtttgc gcgacatcga tctggcaaaa atcctaaaaa cgactatgcg tggctcactt
     2701 tatattaata tgaaaaatac aaacttggtg gtgcaggtgc tgcagaggcc atcgatgaag
     2761 aagtggaggc cttgttgtcg atctacaaaa ttgtatggtt gtttgagatt ttccttgaaa
     2821 cgctctcaat cttggatatg atataaattc tggataagat atgatggata tgatctatgt
     2881 ttgatagagt gcatttgaaa ggataacggc cctgcagaca tttttgaaaa aataacaaac
     2941 atttaatttt ctcactcgtt taggctattc ccctattttt gatattcctt cgcacatatg
     3001 aaaactactt tttttcgaaa ctgttaactc cagaatttat aaatctatag cccttacttg
     3061 attatttatt atcatggtta ctcacctcat gtctctttct cttttcaaga agtcttcgtt
     3121 catatctgtt gtctctttcc tccttttgaa tctctgcttt gctcttcgcc attgtttcct
     3181 gaaaataatg taacttgaat tgtgtaatat acttttttaa tttgaatttg gcttgtaacg
     3241 cggaatcact acgtgcggga tcatttctta ctagaaaacc cagaaaatgc catatttcac
     3301 ttatctcggg gtcatttcta attagaaaag ctacaaaaac catttctaat tacggtgact
     3361 ttagaactgg tcgaaatagc taattagaaa tgggtttttg ctaattagag gtgactctat
     3421 gttatctgag taagcctcgt ttgtatgtaa actcacagta ctaataaatg caagacaccc
     3481 gggtttgtct agatatgaaa taattgaaat atcaattctg acagacaata atggtaatct
     3541 tgataaggag ttccacgccc aggagaacac gttagttttc ttgtttttga ttgcgtgcgt
     3601 tattttggag aaaaactcga ttttttacaa aataattttt tgaaaggaac actgttcaat
     3661 aagttttgtc ttttttctca gttgtgatac ggttttttat tcttttttgt agttatacag
     3721 aagaccgtta cgaaacagac tgatatcgaa acaaacactg atcttacttg cacttataat
     3781 acgactcact agttttcctt cctcctctat atatttttat gttttctttt aaaccttcct
     3841 ccatatttct gaattgaatg ttgttgcata ttgtgtgtcc ttatttcatt tccatcatcc
     3901 ttttgtgtgt ttcccccttc caccaattcc ccccacgtcc taatgaaatg aaagcgagaa
     3961 gataaataaa cttgcacatt gtgttcagat gagaggagcg gaatacgaat tgggagacgg
     4021 aaagagaaaa aaaaatgttt agatttggat tgaattaatt ttttagaaag caagaaaaaa
     4081 aacacaaaga gaataaataa ataataggaa aaaaaacaaa tattcagtat ttaaaatttg
     4141 aaaattcaac gacgttggcg tcgatcatcc ggattacttg tatggccggc tagctcatca
     4201 ggcaccgggc ttgcgggtca tgcaccaggt gcgcggtcct tcgggcacct cgacgtcggc
     4261 ggtgacggtg aagccgagcc gctcgtagaa ggggaggttg cggggcgcgg aggtctccag
     4321 gaaggcgggc accccggcgc gctcggccgc ctccactccg gggagcacga cggcgctgcc
     4381 cagacccttg ccctggtgct gaaaatttaa atcagattag ttagttagta aaatcatgtt
     4441 taaacttacg tcgggcgaga cgccgacggt ggccaggaac cacgcgggct ccttgggccg
     4501 gtgcggcgcc aggaggcctt ccatctgttg ctgcgcggcc agccgggaac cgctcaactc
     4561 ggccatgcgc gggccgatct cggcgaacac cgcccccgct tcgacgctct ccggcgtggt
     4621 ccagaccgcc accgcggcgc cgtcgtccgc tgaaaattta aataatcagg gttagttagt
     4681 atatatatgt ttaaacttac cgacccacac cttgccgatg tcgagcccga cgcgcgtgag
     4741 gaagagttct tgcagctcgg tgacccgctc gatgtggcgg tccggatcga cggtgtggcg
     4801 cgtggcgggg tagtcggcga acgcggcggc gagggtgcgt acggccctgg ggacgtcgtc
     4861 gcgggtggcg aggcgcaccg tgggcttgta ctcggtcatg gtcggtacct ttttttctga
     4921 gccaatcccg gggatccacg agagcgtcgg atattttacc tggaaataaa ttattttatg
     4981 atgaaagtta aatacaataa gatacaacgg gaaacgtaag aaaaattcaa taaaatcaat
     5041 tgtacacgta ggttttcaag caaatttaag aatacaattt tacaaagtcc taaatgtcat
     5101 ttttcctgct caaacttttg actgaaatgc ataaaaattc gctgataaaa acaagcagaa
     5161 tacgccaaaa tagttgcaaa ttataaaatt tacagaatta atacaaaaaa ttcgatattt
     5221 tactcaaaaa aatggaaaaa aagaagaaaa ataaattaaa ctttcgttga gctggcctaa
     5281 aatctaatag caagcgcgct ccattgccaa acggtgtaaa cgcggagcct cgtttgcgtg
     5341 tagtttttat cgtaaatcga cacaacttct ttcaaaataa attatttctt atgagtaatt
     5401 ttctgcattc tattgttgtg tttcaattaa tcttattttt aatttgattt tattcaaaat
     5461 tatataattt tattaagatg ctgggttctt ctctccgcat tttctcccct cgagtgtaaa
     5521 acgacggcca gtgaattatc aactatgtat aataaagttg aacgagaaac gtaaaatgat
     5581 ataaatatca atatattaaa ttagattttg cataaaaaac agactacata atactgtaaa
     5641 acacaacata tccagtcact atggcggccg cattaggcac cccaggcttt acactttatg
     5701 cttccggctc gtataatgtg tggattttga gttaggatcc gtcgagattt tcaggagcta
     5761 aggaagctaa aatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc
     5821 atcgtaaaga acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg
     5881 ttcagctgga tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc
     5941 cggcctttat tcacattctt gcccgcctga tgaatgctca tccggaattc cgtatggcaa
     6001 tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttccatg
     6061 agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc cggcagtttc
     6121 tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag
     6181 ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc accagttttg
     6241 atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg ggcaaatatt
     6301 atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat gccgtttgtg
     6361 atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat gagtggcagg
     6421 gcggggcgta atctagagga tccggcttac taaaagccag ataacagtat gcgtatttgc
     6481 gcgctgattt ttgcggtata agaatatata ctgatatgta tacccgaagt atgtcaaaaa
     6541 gaggtatgct atgaagcagc gtattacagt gacagttgac agcgacagct atcagttgct
     6601 caaggcatat atgatgtcaa tatctccggt tcggtaagca caaccatgca gaatgaagcc
     6661 cgtcgtctgc gtgccgaacg ctggaaagcg gaaaatcagg aagggatggc tgaggtcgcc
     6721 cggtttattg aaatgaacgg ctcttttgcc gacgagaaca ggggctggtg aaatgcagtt
     6781 taaggtttac acctataaaa gagagagccg ttatcgtctg tttgtggatg tacagagtga
     6841 tattattgac acgcccgggc gacggatggt gatccccctg gccagtgcac gtctgctgtc
     6901 agataaagtc ccccgtgaac tttacccggt ggtgcatatc ggggatgaaa gctggcgcat
     6961 gatgaccacc gatatggcca gtgtgccggt ctccgttatc ggggaagaag tggctgatct
     7021 cagccaccgc gaaaatgaca tcaaaaacgc cattaacctg atgttctggg gaatataaat
     7081 gtcaggctcc cttatacaca gccagtctgc aggtcgacca tagtgactgg atatgttgtg
     7141 ttttacagta ttatgtagtc tgttttttat gcaaaatcta atttaatata ttgatattta
     7201 tatcatttta cgtttctcgt tcaacttttc tatacaaagt tgatagcttg gcgtaatcat
     7261 ggtcatagct gttcctgcct aggtgagctc tggtaccctc tagtcaaggc cttaagtgag
     7321 tcgtattacg tagcccgggc ctagttctag acattctcta atgaaaaaat ctttcagttg
     7381 aaattgaaaa tgagttaaag ttggagtttt tattgaaaac agatttccgt gtgattagtg
     7441 tttttagcga gtgtgacagg acagcgaaaa aatatagaaa caagggggga actgaaaagc
     7501 ttaggaatgc attgaacatg agaaggggaa ggggaaggaa caaactagac aggaattatt
     7561 ggaatttaat cacatttgga gttttttttc tattcgacag aataattatc cagaacattt
     7621 ttgtattaaa tatttatgca tcatatgagt agtcggcttt gttgtgcatg acgagtttgt
     7681 tatcgacgaa atagaagctg tcagaacgag tctcgtttgg attgttgatc atgtcgtcca
     7741 ctgaaaaaga gattagtctt ttgaattgta ctttttagaa taatgactca ctgagttgtt
     7801 gagagagttg agggaactca tagatatgtt cacagttgtt tcgtgaattc ggaatacaga
     7861 atccgaattc aaagtcaaaa cacttcagaa ggcgatcttt gaagaagtga cgttcgatca
     7921 ttcggaaatg atggattggg atgtctccta ctttgaattc cacagttgct ccgaccgttt
     7981 tgagtttcaa aaagtttgga gcaaatctat aacgcacgta tcttgccgac tcctgtggcg
     8041 actcatcatt ctcctgatcg ttctccggtt tggcgatctc gaaaagcact tgctcagtgt
     8101 ccagatcacg gatttggaac ttggtgaact cgatgttata gatgttcgca gatggggagc
     8161 ataagaatcc taaatttatg ttttaaactg aaatccaaag ggagcaagat accttgagtg
     8221 attcccggaa gtgctaaaac gtcgttcgga gtgatttgag ctttcttcgc aagctccgat
     8281 tccgttgtga ttccttgttc ggtgcttggt ggtggccgtg gcatctggaa atatggaaaa
     8341 gttcaacaaa aagaaaagag aaaagaatga aatcggatat caagagttag ttgagcggtt
     8401 tctctagttt tctgagtctc acctgcgacg ggaaggtcgc cgagccgggt ggaatcgatc
     8461 gttgttgctc ggctttcata tcggtttggt tggaagcggc tgaaaacgga aagaagtgga
     8521 agaaggaaaa gagtgtggtg tgacaggaaa atggtaatta gagggtgcca aataaccagc
     8581 tatattttgt ttttttttga aaacattttt aaaaagaaaa atacgataat gatatcagat
     8641 ggatttccgg aaaactggta tgaaaaattt caaccttttt gagtacatgt aatcaaaata
     8701 cactttgtaa attatcattt ttattgaaac tccaccattt ttctatttat aacgctaata
     8761 atttgaaaaa gaaacctatt gcgaaccgcg gggtgaatcc caaaaacgaa tgcgttttgg
     8821 tggagtgatt gattcgaatc gaagaagaaa aagaagaaga cgtggaatag agagctcact
     8881 cttaaccgag cagcacacac cgacagaaaa aaaaatgaaa tgaatgaggg tcttcttctt
     8941 cttcttcttc gaatgattga cagaaatggg aaaaagagga agattgagaa gggaaaaagg
     9001 aaggagaaaa gaagcagaag aagacgtcag agaggagagg aacgagcgga aaagcagcgg
     9061 gcgcaagtca tagaagtagc agagctgggg agaagaagac actatccaag aaaggaatga
     9121 cgagagagta tgcaaagggg tatagggtgc agacagaata ggaacagaat aacagatgat
     9181 gagccaagaa gagttgaaaa gggcgatgaa tttgtcatgt aacttaattt gggtcaattt
     9241 gagcatgatg aattgaaatc atcccttgtt gggagttaat aaccggtttg ttatcagaaa
     9301 ccctgtaata gaagggcgcc ctaactttga gccaattcat cccggtttct gtcaaatata
     9361 tcaaaaagtg gtcaactgac aaattgtttt tgatattata ataaacattt tatccgttaa
     9421 caattttcga atacttttta caaggacttg gataaattgg ctcaaagacg aagtgagttt
     9481 gctaccatca tggaatcagg aggacatttc gacaatgtca tataatgttt gaaaaatgat
     9541 tttaacaagg gtctcattcg agataagcaa tgctctgagc aagtggctcg tcgtcttcaa
     9601 gaatctcttg ttgatattca tttggatcct gttttgcacg aagcatgtgc catggatatt
     9661 cagagttttt gcaaaaaagt tccgccaggt caaacaatct ttttaatttt tatatgatac
     9721 ttcgggtgta tttttcttct ttttcctgtt cattttcgct tttttctcac ttttttgtaa
     9781 caaaatctct cacttttctg taagtcttaa caaaagttca attggctcga tcaagtaaaa
     9841 tttcggccgt atgaacgttt tcaaaggttt tcattttttt ttaagttcat tcacaatatc
     9901 atgaatctct cgtcctttgg tttccggtaa gtagcgaaat aatataagca tacttataaa
     9961 tgaaggaatt gaatatagaa taaggaaagc ataactacca ataacggaat atagaggtaa
    10021 tactgtaaat gtactgataa ccaccattac agtattcatt gaatatgcca cagattgagc
    10081 aatactacga tgtttttgag gtaccagttc agaggatata aaccacgaaa ttggaccaac
    10141 tccagttctg aaatttcatt attcgattaa atcaaaatat atttgaaaaa cctcacccat
    10201 aagtataacc gtagatgatc aatgcaacta ggcatccgta tttcatcggg tcaatcagaa
    10261 tacgtatttt tgcgaagaat acgaatgcaa ttagagctaa attattgaga aatgtgaaga
    10321 gaaggagaag cttgcgtcga ccaactctgt aaacttattc atacaatttt gtcttcaaat
    10381 tttgaacgtt taaaactatc ttattcaatg agttagcggg tttttatagt gagttagttc
    10441 acgattatct aataaaacta tgtccaaaca tcctaactgc gaccgtattc tcactaactc
    10501 gtcttaaaaa cgttgagatt attttaaacc cttcttcagt acaactacag tactgatgca
    10561 caaatacaat aataataata cattcagaca tactctgaac aatgtaactt gtttttgtta
    10621 tactgtggta acatttattg aaatgaaata taaccttact tttcaatcac aattccacca
    10681 gtaattgttc cagaaacata agctaaaccc attgcagttg tactccattc agcaatttct
    10741 gactcaacat ttgcattttc tagaaagaaa gtagaggata aaagaagagc ccataatccg
    10801 acagggccc
//
