LOCUS       sequence-12431-e       12445 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12445)
  AUTHORS   Semple JI, Garcia-Verdugo R, Lehner B
  TITLE     Rapid selection of transgenic C. elegans using antibiotic 
            resistance.
  JOURNAL   Nat Methods. 2010 Sep;7(9):725-7. doi: 10.1038/nmeth.1495. Epub 2010
            Aug 22.
  PUBMED    20729840
REFERENCE   2  (bases 1 to 12445)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1495"; journalName: "Nat Methods"; date: "2010-09"; 
            volume: "7"; issue: "9"; pages: "725-7"
COMMENT     Sequence Label: pBCN27-Pmyo-2::GFP::unc-54_3'UTR
FEATURES             Location/Qualifiers
     source          1..12445
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(146..734)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(226..245)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(905..1696,1697..1765))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1528..1547
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1766..1870)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1938..1956
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     2116..2135
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4281..4301)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     4880..4899
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5515..5532
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5516..5532
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5540..5560
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    6925..6949
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(6993..7013)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     7761..7789
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     protein_bind    complement(7840..7864)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(8858..8878)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             10944..10955
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 tgtgaaattg ttatccgctg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
       61 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag accaggaacc
      121 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
      181 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
      241 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
      301 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
      361 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
      421 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
      481 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
      541 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
      601 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
      661 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
      721 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
      781 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
      841 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
      901 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
      961 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     1021 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     1081 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     1141 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     1201 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     1261 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     1321 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     1381 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     1441 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     1501 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     1561 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     1621 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     1681 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     1741 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     1801 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     1861 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
     1921 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg
     1981 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
     2041 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
     2101 gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg
     2161 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc
     2221 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
     2281 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
     2341 gtcacgtgtg tttttgaaca cggcgatatg tatctgtaga tcatccccaa ttttcaacaa
     2401 tgaatctaaa tactgaactt tttgtgtaat ctttagtgca gaaaaatgac caaaatcagc
     2461 gattttccga ttatacccaa aatatctcag ctcaatgagc acctataaaa atgtaaaaat
     2521 aaagaaagta gtcgaaccat attttgtaaa ggctaaaaaa ctgaataaat ttttatccat
     2581 ccagcgcggc gttacaatcc gtggcccatg tcacgcacgg ctttcaaaga aaaaaaaaca
     2641 gtctgtttgc gcgacatcga tctggcaaaa atcctaaaaa cgactatgcg tggctcactt
     2701 tatattaata tgaaaaatac aaacttggtg gtgcaggtgc tgcagaggcc atcgatgaag
     2761 aagtggaggc cttgttgtcg atctacaaaa ttgtatggtt gtttgagatt ttccttgaaa
     2821 cgctctcaat cttggatatg atataaattc tggataagat atgatggata tgatctatgt
     2881 ttgatagagt gcatttgaaa ggataacggc cctgcagaca tttttgaaaa aataacaaac
     2941 atttaatttt ctcactcgtt taggctattc ccctattttt gatattcctt cgcacatatg
     3001 aaaactactt tttttcgaaa ctgttaactc cagaatttat aaatctatag cccttacttg
     3061 attatttatt atcatggtta ctcacctcat gtctctttct cttttcaaga agtcttcgtt
     3121 catatctgtt gtctctttcc tccttttgaa tctctgcttt gctcttcgcc attgtttcct
     3181 gaaaataatg taacttgaat tgtgtaatat acttttttaa tttgaatttg gcttgtaacg
     3241 cggaatcact acgtgcggga tcatttctta ctagaaaacc cagaaaatgc catatttcac
     3301 ttatctcggg gtcatttcta attagaaaag ctacaaaaac catttctaat tacggtgact
     3361 ttagaactgg tcgaaatagc taattagaaa tgggtttttg ctaattagag gtgactctat
     3421 gttatctgag taagcctcgt ttgtatgtaa actcacagta ctaataaatg caagacaccc
     3481 gggtttgtct agatatgaaa taattgaaat atcaattctg acagacaata atggtaatct
     3541 tgataaggag ttccacgccc aggagaacac gttagttttc ttgtttttga ttgcgtgcgt
     3601 tattttggag aaaaactcga ttttttacaa aataattttt tgaaaggaac actgttcaat
     3661 aagttttgtc ttttttctca gttgtgatac ggttttttat tcttttttgt agttatacag
     3721 aagaccgtta cgaaacagac tgatatcgaa acaaacactg atcttacttg cacttataat
     3781 acgactcact agttttcctt cctcctctat atatttttat gttttctttt aaaccttcct
     3841 ccatatttct gaattgaatg ttgttgcata ttgtgtgtcc ttatttcatt tccatcatcc
     3901 ttttgtgtgt ttcccccttc caccaattcc ccccacgtcc taatgaaatg aaagcgagaa
     3961 gataaataaa cttgcacatt gtgttcagat gagaggagcg gaatacgaat tgggagacgg
     4021 aaagagaaaa aaaaatgttt agatttggat tgaattaatt ttttagaaag caagaaaaaa
     4081 aacacaaaga gaataaataa ataataggaa aaaaaacaaa tattcagtat ttaaaatttg
     4141 aaaattcaac gacgttggcg tcgatcatcc ggattacttg tatggccggc tagctcatca
     4201 ggcaccgggc ttgcgggtca tgcaccaggt gcgcggtcct tcgggcacct cgacgtcggc
     4261 ggtgacggtg aagccgagcc gctcgtagaa ggggaggttg cggggcgcgg aggtctccag
     4321 gaaggcgggc accccggcgc gctcggccgc ctccactccg gggagcacga cggcgctgcc
     4381 cagacccttg ccctggtgct gaaaatttaa atcagattag ttagttagta aaatcatgtt
     4441 taaacttacg tcgggcgaga cgccgacggt ggccaggaac cacgcgggct ccttgggccg
     4501 gtgcggcgcc aggaggcctt ccatctgttg ctgcgcggcc agccgggaac cgctcaactc
     4561 ggccatgcgc gggccgatct cggcgaacac cgcccccgct tcgacgctct ccggcgtggt
     4621 ccagaccgcc accgcggcgc cgtcgtccgc tgaaaattta aataatcagg gttagttagt
     4681 atatatatgt ttaaacttac cgacccacac cttgccgatg tcgagcccga cgcgcgtgag
     4741 gaagagttct tgcagctcgg tgacccgctc gatgtggcgg tccggatcga cggtgtggcg
     4801 cgtggcgggg tagtcggcga acgcggcggc gagggtgcgt acggccctgg ggacgtcgtc
     4861 gcgggtggcg aggcgcaccg tgggcttgta ctcggtcatg gtcggtacct ttttttctga
     4921 gccaatcccg gggatccacg agagcgtcgg atattttacc tggaaataaa ttattttatg
     4981 atgaaagtta aatacaataa gatacaacgg gaaacgtaag aaaaattcaa taaaatcaat
     5041 tgtacacgta ggttttcaag caaatttaag aatacaattt tacaaagtcc taaatgtcat
     5101 ttttcctgct caaacttttg actgaaatgc ataaaaattc gctgataaaa acaagcagaa
     5161 tacgccaaaa tagttgcaaa ttataaaatt tacagaatta atacaaaaaa ttcgatattt
     5221 tactcaaaaa aatggaaaaa aagaagaaaa ataaattaaa ctttcgttga gctggcctaa
     5281 aatctaatag caagcgcgct ccattgccaa acggtgtaaa cgcggagcct cgtttgcgtg
     5341 tagtttttat cgtaaatcga cacaacttct ttcaaaataa attatttctt atgagtaatt
     5401 ttctgcattc tattgttgtg tttcaattaa tcttattttt aatttgattt tattcaaaat
     5461 tatataattt tattaagatg ctgggttctt ctctccgcat tttctcccct cgagtgtaaa
     5521 acgacggcca gtgaattatc aactatgtat aataaagttg acttgtctgc tcccggcatc
     5581 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
     5641 atcaccgaaa cgcgcgagac gaaagggccc gtacggccga ctagtggatc ccccaacggt
     5701 ggagtagcgt ctctggcttt tgccctaaat gacagaatac agtcccacta taactattaa
     5761 aaaatttttc taaatgtttt acgattttcc aaaaaaataa aaaaggccga attattcttg
     5821 atttcccgcg cagatgagtg acgtcatttt cgacattttt gcgttttatg gccaaactaa
     5881 taggtttttc ttcatttttt tctttgtttt ttatttgaaa aataattttc caatttttcc
     5941 agattaatca tattttttct ttagggaaat atgttcgaaa atttacaaat tggagttcga
     6001 atcataaaat aaagaaaaac acaaacaaat acctattaat atagccggaa aacacaataa
     6061 tgcagaaaat gaagtcactc atttgcgcgg gaattcaaat ttaagtaatt tttgcaaatt
     6121 taaaagaaat cataaaaaat ttagaaaaat gtttaatttt aaacagttat gtttggtata
     6181 ttgggaatgt attctgtcat ttaaggcaaa aaccccatag acactactcc actttcaaat
     6241 tatcaacttc tactcacctt ccactgagcc tcaaacccaa accttcttcc gatctttctt
     6301 gcatcgtgct catcaatact tgtgatttta gcaagaaaaa cgccgtcctc gaaatcttga
     6361 caaacaatag gggaaacccc aaaaagcaaa agcaggaaaa ttaatttcat aaactttcac
     6421 ttgattgaaa aattccaaaa atactccaaa actttttcac gatgagcatg atttgacgtc
     6481 atgagaggcc cagacgtgcg aagaaataaa aattgcggtc ataaactgaa acgtaacata
     6541 tgataaggta ttttgtgtgc ggttttttct atgatgtttc aaaaaaattt atcagaagta
     6601 aaaaacataa gaagtgtaca cagaacattg tgtagatttt gggatagaat tattattaat
     6661 ggggggaggg agcacaattt ttttcgactt tttattacga attaattcta tttttgaatc
     6721 tacacaattt cattgttaga ggtgacttaa aagaagctaa aaaacaaaga aattaagaga
     6781 agttttttga taataacaaa aatagggggt gggagcacag ggagaaagag catgtaggga
     6841 tgttgaagag taattggact tagaagtcag aggcacgggc gcgagatggc gatctgatga
     6901 cagcggccga tgttagttag ttaggccact ttgtacaaga aagctgggtc tatttgtata
     6961 gttcatccat gccatgtgta atcccagcag ctgttacaaa ctcaagaagg accatgtggt
     7021 ctctcttttc gttgggatct ttcgaaaggg cagattgtgt ggacaggtaa tggttgtctg
     7081 gtaaaaggac agggccatcg ccaattggag tattttgttg ataatggtct gctagttgaa
     7141 cgcttccatc ttcaatgttg tgtctaattt tgaagttctg aaaatttaaa tcagattagt
     7201 tagttagtaa aatcatgttt aaacttacaa ctttgattcc attcttttgt ttgtctgcca
     7261 tgatgtatac attgtgtgag ttatagttgt attccaattt gtgtccaaga atgtttccat
     7321 cttctttaaa atcaatacct tttaactcga ttctattaac aagggtatca ccttcaaact
     7381 tgacttcagc acctgaaaat ttaaatatgt atggttagtt agtaccgaac tgtttaaact
     7441 tacgtgtctt gtagttcccg tcatctttga aaaatatagt tctttcctgt acataacctt
     7501 cgggcatggc actcttgaaa aagtcatgcc gtttcatatg atctgggtat ctcgagaagc
     7561 attgaacacc ataacagaaa gtagtgacaa gtgttggctg aaaatttaaa taatcagggt
     7621 tagttagtat atatatgttt aaacttaccc atggaacagg tagttttcca gtagtgcaaa
     7681 taaatttaag ggtaagtttt ccgtatgttg catcaccttc accctctcca ctgacagaaa
     7741 atttgtgccc attaacatca ccatctaatt caacaagaat tgggacaact ccagtgaaaa
     7801 gttcttctcc tttactcatg gtgaaggggg cggccgcgga gcctgctttt ttgtacaaac
     7861 ttgggtcgaa ttcgcccttt ttctgtgtct gacgatcgag ggttaaaatg aaaagtggtg
     7921 gagagaagtc aaccgctcgg tgggatgcgc gagcctggag ataactttga ttttcggcga
     7981 gcccttcttt ttccctgggt gggtggattg ggaggcgggg atatccaatt gtaattctag
     8041 gtagctgaaa agtgtctgca cactgttctc taaccatcga cgcaggaaaa gtctctttct
     8101 caagtgcaaa tagcaaaaat cttctttctt cccccccatc ttccgagcaa ggcgccccca
     8161 cggatagttg ctcgctggcg gtagggagcg aagagagcag agaacctcaa cgggatagtg
     8221 gggatacgag aaaagaaacg gcattttagg ttaactaaag atagtgaaga agccggctca
     8281 atgcagagag gcagacatcc gacggcaaac aacgagagaa ttttatccag aacaatgagt
     8341 acagtgtgtg cgcgcaaagt gctcttcctg ccattttgct actcttatcc acacaaccac
     8401 tttagcgatt accaacctat cttgagactt atctttattt tctcttcttt tgccgacgaa
     8461 acgggagaga ggggcaaaaa atgaagaatc gggggcactg attaacacac ttactttttt
     8521 ttaggctgaa gcacacattg ctgttcgggg tgtaaagttc agcagaattc aaagcaaaag
     8581 accacccgca tcggtagtca atattgatct ggaagattat taaaaattgt cagagtgagc
     8641 catatgcata tgaatttaat tcatacataa catttataag acaggaataa ataccaaagt
     8701 gaagttatta tttggtcgat tatcacttta gcatagtgaa agtatccctc aatgtctcta
     8761 cttgtaaact cagttgattt acaaacgttg ttttattgat tataaaatta gttttatgga
     8821 catttaaagc aaaggatact actcagatat aaaatggcaa cttttctata caaagttgat
     8881 agcttggcgt aatcatggtc atagctgttc ctgcctaggt gagctctggt accctctagt
     8941 caaggcctta agtgagtcgt attacgtagc ccgggcctag ttctagacat tctctaatga
     9001 aaaaatcttt cagttgaaat tgaaaatgag ttaaagttgg agtttttatt gaaaacagat
     9061 ttccgtgtga ttagtgtttt tagcgagtgt gacaggacag cgaaaaaata tagaaacaag
     9121 gggggaactg aaaagcttag gaatgcattg aacatgagaa ggggaagggg aaggaacaaa
     9181 ctagacagga attattggaa tttaatcaca tttggagttt tttttctatt cgacagaata
     9241 attatccaga acatttttgt attaaatatt tatgcatcat atgagtagtc ggctttgttg
     9301 tgcatgacga gtttgttatc gacgaaatag aagctgtcag aacgagtctc gtttggattg
     9361 ttgatcatgt cgtccactga aaaagagatt agtcttttga attgtacttt ttagaataat
     9421 gactcactga gttgttgaga gagttgaggg aactcataga tatgttcaca gttgtttcgt
     9481 gaattcggaa tacagaatcc gaattcaaag tcaaaacact tcagaaggcg atctttgaag
     9541 aagtgacgtt cgatcattcg gaaatgatgg attgggatgt ctcctacttt gaattccaca
     9601 gttgctccga ccgttttgag tttcaaaaag tttggagcaa atctataacg cacgtatctt
     9661 gccgactcct gtggcgactc atcattctcc tgatcgttct ccggtttggc gatctcgaaa
     9721 agcacttgct cagtgtccag atcacggatt tggaacttgg tgaactcgat gttatagatg
     9781 ttcgcagatg gggagcataa gaatcctaaa tttatgtttt aaactgaaat ccaaagggag
     9841 caagatacct tgagtgattc ccggaagtgc taaaacgtcg ttcggagtga tttgagcttt
     9901 cttcgcaagc tccgattccg ttgtgattcc ttgttcggtg cttggtggtg gccgtggcat
     9961 ctggaaatat ggaaaagttc aacaaaaaga aaagagaaaa gaatgaaatc ggatatcaag
    10021 agttagttga gcggtttctc tagttttctg agtctcacct gcgacgggaa ggtcgccgag
    10081 ccgggtggaa tcgatcgttg ttgctcggct ttcatatcgg tttggttgga agcggctgaa
    10141 aacggaaaga agtggaagaa ggaaaagagt gtggtgtgac aggaaaatgg taattagagg
    10201 gtgccaaata accagctata ttttgttttt ttttgaaaac atttttaaaa agaaaaatac
    10261 gataatgata tcagatggat ttccggaaaa ctggtatgaa aaatttcaac ctttttgagt
    10321 acatgtaatc aaaatacact ttgtaaatta tcatttttat tgaaactcca ccatttttct
    10381 atttataacg ctaataattt gaaaaagaaa cctattgcga accgcggggt gaatcccaaa
    10441 aacgaatgcg ttttggtgga gtgattgatt cgaatcgaag aagaaaaaga agaagacgtg
    10501 gaatagagag ctcactctta accgagcagc acacaccgac agaaaaaaaa atgaaatgaa
    10561 tgagggtctt cttcttcttc ttcttcgaat gattgacaga aatgggaaaa agaggaagat
    10621 tgagaaggga aaaaggaagg agaaaagaag cagaagaaga cgtcagagag gagaggaacg
    10681 agcggaaaag cagcgggcgc aagtcataga agtagcagag ctggggagaa gaagacacta
    10741 tccaagaaag gaatgacgag agagtatgca aaggggtata gggtgcagac agaataggaa
    10801 cagaataaca gatgatgagc caagaagagt tgaaaagggc gatgaatttg tcatgtaact
    10861 taatttgggt caatttgagc atgatgaatt gaaatcatcc cttgttggga gttaataacc
    10921 ggtttgttat cagaaaccct gtaatagaag ggcgccctaa ctttgagcca attcatcccg
    10981 gtttctgtca aatatatcaa aaagtggtca actgacaaat tgtttttgat attataataa
    11041 acattttatc cgttaacaat tttcgaatac tttttacaag gacttggata aattggctca
    11101 aagacgaagt gagtttgcta ccatcatgga atcaggagga catttcgaca atgtcatata
    11161 atgtttgaaa aatgatttta acaagggtct cattcgagat aagcaatgct ctgagcaagt
    11221 ggctcgtcgt cttcaagaat ctcttgttga tattcatttg gatcctgttt tgcacgaagc
    11281 atgtgccatg gatattcaga gtttttgcaa aaaagttccg ccaggtcaaa caatcttttt
    11341 aatttttata tgatacttcg ggtgtatttt tcttcttttt cctgttcatt ttcgcttttt
    11401 tctcactttt ttgtaacaaa atctctcact tttctgtaag tcttaacaaa agttcaattg
    11461 gctcgatcaa gtaaaatttc ggccgtatga acgttttcaa aggttttcat ttttttttaa
    11521 gttcattcac aatatcatga atctctcgtc ctttggtttc cggtaagtag cgaaataata
    11581 taagcatact tataaatgaa ggaattgaat atagaataag gaaagcataa ctaccaataa
    11641 cggaatatag aggtaatact gtaaatgtac tgataaccac cattacagta ttcattgaat
    11701 atgccacaga ttgagcaata ctacgatgtt tttgaggtac cagttcagag gatataaacc
    11761 acgaaattgg accaactcca gttctgaaat ttcattattc gattaaatca aaatatattt
    11821 gaaaaacctc acccataagt ataaccgtag atgatcaatg caactaggca tccgtatttc
    11881 atcgggtcaa tcagaatacg tatttttgcg aagaatacga atgcaattag agctaaatta
    11941 ttgagaaatg tgaagagaag gagaagcttg cgtcgaccaa ctctgtaaac ttattcatac
    12001 aattttgtct tcaaattttg aacgtttaaa actatcttat tcaatgagtt agcgggtttt
    12061 tatagtgagt tagttcacga ttatctaata aaactatgtc caaacatcct aactgcgacc
    12121 gtattctcac taactcgtct taaaaacgtt gagattattt taaacccttc ttcagtacaa
    12181 ctacagtact gatgcacaaa tacaataata ataatacatt cagacatact ctgaacaatg
    12241 taacttgttt ttgttatact gtggtaacat ttattgaaat gaaatataac cttacttttc
    12301 aatcacaatt ccaccagtaa ttgttccaga aacataagct aaacccattg cagttgtact
    12361 ccattcagca atttctgact caacatttgc attttctaga aagaaagtag aggataaaag
    12421 aagagcccat aatccgacag ggccc
//
