LOCUS       sequence-124310-         829 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of PABPC1-MS2 fusion, double mutant..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Fatscher T, Boehm V, Weiche B, Gehring NH
  TITLE     The interaction of cytoplasmic poly(A)-binding protein with 
            eukaryotic initiation factor 4G suppresses nonsense-mediated mRNA 
            decay.
  JOURNAL   RNA. 2014 Oct;20(10):1579-92. doi: 10.1261/rna.044933.114. Epub 2014
            Aug 21.
  PUBMED    25147240
REFERENCE   2  (bases 1 to 829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.044933.114"; journalName: "RNA"; date: "2014-10"; 
            volume: "20"; issue: "10"; pages: "1579-92"
COMMENT     Sequence Label: pCI-MS2V5-PABPC1 MLLEmut M161A/D165K
FEATURES             Location/Qualifiers
     source          1..829
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        14..32
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             44..430
                     /codon_start=1
                     /product="bacteriophage MS2 coat protein"
                     /label=MS2
                     /note="binds to a specific stem-loop structure in the viral
                     RNA (Peabody, 1993)"
                     /translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
                     CSVRQSSAQNRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
                     VKAMQGLLKDGNPIPSAIAANSGIY"
     CDS             437..478
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 aggctagagt acttaatacg actcactata ggctagcacc atggcttcta actttactca
       61 gttcgttctc gtggacaatg gcggaactgg cgacgtgact gtcgccccaa gcaacttcgc
      121 taacggggtc gctgaatgga tcagctctaa ctcgcgttca caggcttaca aagtaacctg
      181 tagcgttcgt cagagctctg cgcagaatcg caaatacacc atcaaagtcg aggtgcctaa
      241 agtggcaacc cagactgttg gtggtgtaga gcttcctgta gccgcatggc gttcgtactt
      301 aaatatggaa ctaaccattc caattttcgc cacgaactcc gactgcgagc ttattgttaa
      361 ggcaatgcaa ggtctcctaa aagatggaaa cccgattccc tcagcaatcg cagcaaactc
      421 cggcatctac ggcggtggca agcccatccc taacccactg ctgggcctgg acagcaccct
      481 cgagaacccc agtgccccca gctacccgat ggcctcgctc tacgtggggg acctccaccc
      541 cgacgtgacc gaggcgatgc tctacgagaa gttcagcccg gccgggccca tcctctccat
      601 ccgggtctgc agggacatga tcacccgccg ctccttgggc tacgcgtatg tgaacttcca
      661 gcagccggcg gacgcggagc gtgctttgga caccatgaat tttgatgtta taaagggcaa
      721 gccagtacgc atcatgtggt ctcagcgtga tccatcactt cgcaaaagtg gagtaggcaa
      781 catattcatt aaaaatctgg acaaatccat tgataataaa gcactgtat
//
