LOCUS       sequence-124315-         884 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  construction of CYC1 promoter in HCKan-P receiving vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Guo Y, Dong J, Zhou T, Auxillos J, Li T, Zhang W, Wang L, Shen Y, 
            Luo Y, Zheng Y, Lin J, Chen GQ, Wu Q, Cai Y, Dai J
  TITLE     YeastFab: the design and construction of standard biological parts 
            for metabolic engineering in Saccharomyces cerevisiae.
  JOURNAL   Nucleic Acids Res. 2015 May 8. pii: gkv464.
  PUBMED    25956650
REFERENCE   2  (bases 1 to 884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 May 8. pii: gkv464."
COMMENT     Sequence Label: HCKan-P-CYC1
FEATURES             Location/Qualifiers
     source          1..884
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(43..145)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      complement(146..177)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     terminator      567..596
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
ORIGIN
        1 tcgcggcctc gagcaagacg tttcccgttg aatatggctc attttagctt ccttagctcc
       61 tgaaaatctc gataactcaa aaaatacgcc cggtagtgat cttatttcat tatggtgaaa
      121 gttggaacct cttacgtgcc gatcaagtca aaagcctccg gtcggaggct tttgactttc
      181 tgctatggag gtcaggtatg atttaaatgg tcagtcgtct cgcatcttag tgtgtgtatt
      241 tgtgtttgcg tgtctataga agtatagtaa tttatgctgc aaaggtccta atgtataagg
      301 aaagaatatt tagagaaaag aagaaaacaa gagttttata tacatacaga gcacatgcat
      361 gccatatgat catgtgtcgt cgcacacata tatatatgcc tgtatgtgtc agcactaaag
      421 ttgcctggcc atccacgcta tatatacacg cctggcggat ctgctcgagg attgcctacg
      481 cgtgggcttg atccaccaac caacgctcgc caaatgagct cagcccgaga cggactccag
      541 cgtaactgga ctgcaatcaa ctcactggct caccttcacg ggtgggcctt tcttcggtag
      601 aaaatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
      661 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
      721 ccgaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
      781 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
      841 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct tacc
//
