LOCUS       sequence-124318-         808 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  systhesis of vioE gene in the violacein pathway.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 808)
  AUTHORS   Guo Y, Dong J, Zhou T, Auxillos J, Li T, Zhang W, Wang L, Shen Y, 
            Luo Y, Zheng Y, Lin J, Chen GQ, Wu Q, Cai Y, Dai J
  TITLE     YeastFab: the design and construction of standard biological parts 
            for metabolic engineering in Saccharomyces cerevisiae.
  JOURNAL   Nucleic Acids Res. 2015 May 8. pii: gkv464.
  PUBMED    25956650
REFERENCE   2  (bases 1 to 808)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 May 8. pii: gkv464."
COMMENT     Sequence Label: pKMV-VioE
FEATURES             Location/Qualifiers
     source          1..808
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(603..620)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(603..619)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(629..647)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 aacagcgtct gggatttcag cttcagaaac gtttgggaag tctctcaaag aagctctact
       61 agcctcgtca ccggtaacca ttctcaatgg ggtaccagaa gcagcgtcca agtacaaggt
      121 agatggaccc ttacctggtc tttggtatct ccaaccgtca gcttctctac ccaaaacaac
      181 tcttctaccg atgtgtctag cacccaatct tctcaaaacg tcgcgtggca agaacaattc
      241 agcgaatgga ccggtttcgt cgtccaatgg tctttcacac aattgttcac ccaaagcggt
      301 tctttctcta ccgtaagcaa ccttttgctt ccaggttcta ccagaagcag cgttaccaac
      361 ttcagacatc cacaatctgt aaccggtgtc tctttcagac cacgggttga acaaaccgtc
      421 gattctacag atgtctcttt cgtagtcgaa ccaacagtaa ccagaggtca attggtcgtc
      481 tggcaacatt ggactccagt aagaaacgta agcagaagac catctagctg gcaacaatgg
      541 tggttctctg ttttccatca gagacgcaag acactgcgga tcgagacgca ttgcgtggtt
      601 cgactggccg tcgttttaca acgaaagggc ctcgtgatac gcctattttt ataggttaat
      661 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
      721 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
      781 ccctgataaa tgcttcaata atattgaa
//
