LOCUS       sequence-124320-        2640 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  systhesis of vioE gene in the violacein pathway.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Guo Y, Dong J, Zhou T, Auxillos J, Li T, Zhang W, Wang L, Shen Y, 
            Luo Y, Zheng Y, Lin J, Chen GQ, Wu Q, Cai Y, Dai J
  TITLE     YeastFab: the design and construction of standard biological parts 
            for metabolic engineering in Saccharomyces cerevisiae.
  JOURNAL   Nucleic Acids Res. 2015 May 8. pii: gkv464.
  PUBMED    25956650
REFERENCE   2  (bases 1 to 2640)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 May 8. pii: gkv464."
COMMENT     Sequence Label: pKMV-VioE
FEATURES             Location/Qualifiers
     source          1..2640
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     50..68
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     77..94
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     78..94
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(744..760)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(744..760)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(772..789)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(943..1531)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1023..1042)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1702..2517)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(1702..2517)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     2425..2444
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(2518..2622)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
       61 cacgaggccc tttcgttgta aaacgacggc cagtcgaacc acgcaatgcg tctcgatccg
      121 cagtgtcttg cgtctctgat ggaaaacaga gaaccaccat tgttgccagc tagatggtct
      181 tctgcttacg tttcttactg gagtccaatg ttgccagacg accaattgac ctctggttac
      241 tgttggttcg actacgaaag agacatctgt agaatcgacg gtttgttcaa cccgtggtct
      301 gaaagagaca ccggttacag attgtggatg tctgaagttg gtaacgctgc ttctggtaga
      361 acctggaagc aaaaggttgc ttacggtaga gaaagaaccg ctttgggtga acaattgtgt
      421 gaaagaccat tggacgacga aaccggtcca ttcgctgaat tgttcttgcc acgcgacgtt
      481 ttgagaagat tgggtgctag acacatcggt agaagagttg ttttgggtag agaagctgac
      541 ggttggagat accaaagacc aggtaagggt ccatctacct tgtacttgga cgctgcttct
      601 ggtaccccat tgagaatggt taccggtgac gaggctagta gagcttcttt gagagacttc
      661 ccaaacgttt ctgaagctga aatcccagac gctgttttcg ctgctaagag atagcagaga
      721 cggagtcact gccaaccgag acggtcatag ctgtttcctg tgtgccgctt cctcgctcac
      781 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
      841 aatacggtta cccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
      901 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
      961 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     1021 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     1081 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     1141 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     1201 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     1261 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     1321 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     1381 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     1441 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     1501 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     1561 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     1621 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     1681 tgagtaaact tggtctgaca gttagaaaaa ctcatcgagc atcaaatgaa actgcaattt
     1741 attcatatca ggattatcaa taccatattt ttgaaaaagc cgtttctgta atgaaggaga
     1801 aaactcaccg aggcagttcc ataggatggc aagatcctgg tatcggtctg cgattccgac
     1861 tcgtccaaca tcaatacaac ctattaattt cccctcgtca aaaataaggt tatcaagtga
     1921 gaaatcacca tgagtgacga ctgaatccgg tgagaatggc aaaagtttat gcatttcttt
     1981 ccagacttgt tcaacaggcc agccattacg ctcgtcatca aaatcactcg catcaaccaa
     2041 accgttattc attcgtgatt gcgcctgagc gagtcgaaat acgcgatcgc tgttaaaagg
     2101 acaattacaa acaggaatcg aatgcaaccg gcgcaggaac acggccagcg catcaacaat
     2161 attttcacct gaatcaggat attcttctaa tacctggaat gctgttttcc cggggatcgc
     2221 tgtggtgagt aaccatgcat catcaggagt acggataaaa tgcttgatgg tcggaagagg
     2281 cataaattcc gtcagccagt ttagtctgac catctcatct gtaacatcat tggcaacgct
     2341 acctttgcca tgtttcagaa acaactctgg cgcatcgggc ttcccataca atcgatagat
     2401 tgtcgcacct gattgcccga cattatcgcg agcccattta tacccatata aatcagcatc
     2461 catgttggaa tttaatcgcg gcctagagca agacgtttcc cgttgaatat ggctcatact
     2521 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     2581 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
//
