LOCUS       sequence-124333-         761 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive dCas9-VP64 lentiviral expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Kearns NA, Genga RM, Enuameh MS, Garber M, Wolfe SA, Maehr R
  TITLE     Cas9 effector-mediated regulation of transcription and 
            differentiation in human pluripotent stem cells.
  JOURNAL   Development. 2014 Jan;141(1):219-23. doi: 10.1242/dev.103341.
  PUBMED    24346702
REFERENCE   2  (bases 1 to 761)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1242/dev"; 
            journalName: "Development"; date: "2014-01"; volume: "141"; issue: 
            "1"; pages: "219-23"
COMMENT     Sequence Label: pHAGE EF1-alpha dCas9-VP64
FEATURES             Location/Qualifiers
     source          1..761
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(43..69)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    122..146
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(193..342)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             complement(364..453)
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     CDS             complement(454..474)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ctccctaccc ggtagaattt ctagtttaat taatcattac taagcgtagt ctgggacgtc
       61 gtatgggtat tcgaaccgcg ggccctctag actcgagcgg ccgccactgt gctggatatc
      121 aaccactttg tacaagaaag ctgggtctag atatctcgag gcggccgctt atccggattc
      181 gaattagtta atcagcatgt ccaggtcgaa atcatcaagg gcgtcactgc cgagcatgtc
      241 gaggtcaaag tcatcaaggg catccgaacc aagcatgtca aggtcaaaat catcgagggc
      301 gtcacttccc agcatatcca gatcaaaatc gtccaatgcg tcagcccgtc cggaaccgga
      361 tccagcgtaa tctggaacgt cataaggata cgatcctgca tagtccggga cgtcataggg
      421 atagcccgca tagtcaggaa catcgtatgg gtataccttc ctcttcttct tgggcccgcc
      481 ggtgccgtct ccaccgagct gagagaggtc gattcttgtt tcatagagcc ccgtaattga
      541 ctgatgaatc agtgtggcgt ccaggacctc ctttgtagag gtgtaccgct ttctgtctat
      601 ggtggtgtcg aagtacttga aggctgcagg cgcgcccaag ttggtcagag taaacaagtg
      661 gataatgttt tctgcctgct ccctgatggg cttatccctg tgcttattgt aagcagaaag
      721 caccttatcg aggttagcgt cggcgaggat cactcttttg g
//
