LOCUS       sequence-124339-         869 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive dCas9-VP64 lentiviral expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Kearns NA, Genga RM, Enuameh MS, Garber M, Wolfe SA, Maehr R
  TITLE     Cas9 effector-mediated regulation of transcription and 
            differentiation in human pluripotent stem cells.
  JOURNAL   Development. 2014 Jan;141(1):219-23. doi: 10.1242/dev.103341.
  PUBMED    24346702
REFERENCE   2  (bases 1 to 869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1242/dev"; 
            journalName: "Development"; date: "2014-01"; volume: "141"; issue: 
            "1"; pages: "219-23"
COMMENT     Sequence Label: pHAGE EF1-alpha dCas9-VP64
FEATURES             Location/Qualifiers
     source          1..869
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    35..59
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      63..72
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 actagtccag tgtggtggaa ttctgcagat atcaacaagt ttgtacaaaa aagcaggctg
       61 gcgccaccat ggacaagaag tactccattg ggctcgctat cggtaccaac agcgtcggct
      121 gggccgtcat tacggacgag tacaaggtgc cgagcaaaaa attcaaagtt ctgggcaata
      181 ccgatcgcca cagcataaag aagaacctca ttggagccct cctgttcgac tccggggaga
      241 cggccgaagc cacgcggctc aaaagaacag cacggcgcag atatacccgc agaaagaatc
      301 ggatctgcta cctgcaggag atctttagta atgagatggc taaggtggat gactctttct
      361 tccataggct ggaggagtcc tttttggtgg aggaggataa aaagcacgag cgccacccaa
      421 tctttggcaa tatcgtggac gaggtggcgt accatgaaaa gtacccaacc atatatcatc
      481 tgaggaagaa gctggtagac agtactgata aggctgactt gcggttgatc tatctcgcgc
      541 tggcgcacat gatcaaattt cggggacact tcctcatcga gggggacctg aacccagaca
      601 acagcgatgt cgacaaactc tttatccaac tggttcagac ttacaatcag cttttcgagg
      661 agaacccgat caacgcatcc ggcgttgacg ccaaagcaat cctgagcgct aggctgtcca
      721 aatcccggcg gctcgaaaac ctcatcgcac agctccctgg ggagaagaag aacggcctgt
      781 ttggtaatct tatcgccctg tcactcgggc tgacccccaa ctttaaatct aacttcgacc
      841 tggccgaaga tgccaagctg caactgagc
//
