LOCUS       sequence-12434-e        9324 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9324)
  AUTHORS   Giordano-Santini R, Milstein S, Svrzikapa N, Tu D, Johnsen R, 
            Baillie D, Vidal M, Dupuy D
  TITLE     An antibiotic selection marker for nematode transgenesis.
  JOURNAL   Nat Methods. 2010 Sep;7(9):721-3. doi: 10.1038/nmeth.1494. Epub 2010
            Aug 22.
  PUBMED    20729841
REFERENCE   2  (bases 1 to 9324)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1494"; journalName: "Nat Methods"; date: "2010-09"; 
            volume: "7"; issue: "9"; pages: "721-3"
COMMENT     Sequence Label: pdestDD04Neo
FEATURES             Location/Qualifiers
     source          1..9324
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(33..461)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(143..164)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     355..374
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     588..610
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     602..619
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     603..619
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    702..726
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(1658..1678)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     2426..2454
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     CDS             complement(2497..2517)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(2526..2625)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        2782..2884
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             2885..3544
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2951..2970)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             3886..4191
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     4070..4089
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(4581..4704)
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             5540..6334
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5594..5613)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6204..6223
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     7035..7051
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     7036..7052
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7086..7102)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7086..7102)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(7139..7157)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7178..7194)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7178..7194)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7191..7213)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7202..7218
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7226..7232,7233..7250,7251..7256))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7271..7292
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7409..7426)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7580..8168)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7660..7679)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8339..9130,9131..9199))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8962..8981
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9200..9304)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
       61 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
      121 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
      181 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
      241 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
      301 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
      361 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
      421 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc cattcaggct
      481 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
      541 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
      601 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tgggtaccgg
      661 gcccaaagct ggtaccggtc cggaattccc gggtcgagat atcaactttg tacaagaaag
      721 ctgggttacg gccgactagt aggaaacagt tatgtttggt atattgggaa tgtattctgt
      781 catttaaggc aaaaacccca tagacactac tccactttca aattatcaac ttctactcac
      841 cttccactga gcctcaaacc caaaccttct tccgatcttt cttgcatcgt gctcatcaat
      901 acttgtgatt ttagcaagaa aaacgccgtc ctcgaaactc ttgacaaaca ataggggaaa
      961 cccccaaaaa gcaaaagcag gaaaattaat ttcataaact ttcacttgat tgaaaaattc
     1021 caaaaatact ccaaaacttt ttcacgatga gcatgatttg acgtcatgag aggcccagac
     1081 gtgcgaagaa ataaaaattg cggtcataaa ctgaaacgta acatatgata aggtattttg
     1141 tgtgcggttt tttctatgat gtttcaaaaa aaatttatca gaagtaaaaa aaacataaga
     1201 agtgtacaca gaacattgtg tagattttgg gatagaatta ttattaatgg ggggagggag
     1261 cacaattttt ttcgactttt tattacgaat taattctatt tttgaatcta cacaatttca
     1321 ttgttagagg tgacttaaaa gaagctaaaa aacaaagaaa ttaagaagaa gtttttttga
     1381 taataacaaa aatagggggt gggagcacag ggagaaagag catgtaggga tgttgaagag
     1441 taattggact tagaagtcag aggcacgggc gcgagatgct gaaaatttaa atattactcg
     1501 ttagttagta gaactcagtt taaacttact ctgatgacag cggcccctat tatttttgac
     1561 accagacaag ttggtaatgg tagcgaccgg cgctcagttg gaattctacg aatgctattt
     1621 gtatagttca tccatgccat gtgtaatccc agcagctgtt acaaactcaa gaaggaccat
     1681 gtggtctctc ttttcgttgg gatctttcga aagggcagat tgtgtggaca ggtaatggtt
     1741 gtctggtaaa aggacagggc catcgccaat tggagtattt tgttgataat ggtctgctag
     1801 ttgaacgctt ccatcttcaa tgttgtgtct aattttgaag ttctgaaaat ttaaatcaga
     1861 ttagttagtt agtaaaatca tgtttaaact tacaactttg attccattct tttgtttgtc
     1921 tgccatgatg tatacattgt gtgagttata gttgtattcc aatttgtgtc caagaatgtt
     1981 tccatcttct ttaaaatcaa taccttttaa ctcgattcta ttaacaaggg tatcaccttc
     2041 aaacttgact tcagcacctg aaaatttaaa tatgtatggt tagttagtac cgaactgttt
     2101 aaacttacgt gtcttgtagt tcccgtcatc tttgaaaaat atagttcttt cctgtacata
     2161 accttcgggc atggcactct tgaaaaagtc atgccgtttc atatgatctg ggtatctcga
     2221 gaagcattga acaccataac agaaagtagt gacaagtgtt ggctgaaaat ttaaataatc
     2281 agggttagtt agtatatata tgtttaaact tacccatgga acaggtagtt ttccagtagt
     2341 gcaaataaat ttaagggtaa gttttccgta tgttgcatca ccttcaccct ctccactgac
     2401 agaaaatttg tgcccattaa catcaccatc taattcaaca agaattggga caactccagt
     2461 gaaaagttct tctcctttac tcattttttc taccggtacc ttacgcttct tctttggagc
     2521 agtcaagcct gcttttttgt acaaagttgg cattataaaa aagcattgct tatcaatttg
     2581 ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt atttggagct ctagagcgtc
     2641 gactaagttg gcagcatcac ccgacgcact ttgcgccgaa taaatacctg tgacggaaga
     2701 tcacttcgca gaataaataa atcctggtgt ccctgttgat accgggaagc cctgggccaa
     2761 cttttggcga aaatgagacg ttgatcggca cgtaagaggt tccaactttc accataatga
     2821 aataagatca ctaccgggcg tattttttga gttatcgaga ttttcaggag ctaaggaagc
     2881 taaaatggag aaaaaaatca ctggatatac caccgttgat atatcccaat ggcatcgtaa
     2941 agaacatttt gaggcatttc agtcagttgc tcaatgtacc tataaccaga ccgttcagct
     3001 ggatattacg gcctttttaa agaccgtaaa gaaaaataag cacaagtttt atccggcctt
     3061 tattcacatt cttgcccgcc tgatgaatgc tcatccggaa ttccgtatgg caatgaaaga
     3121 cggtgagctg gtgatatggg atagtgttca cccttgttac accgttttcc atgagcaaac
     3181 tgaaacgttt tcatcgctct ggagtgaata ccacgacgat ttccggcagt ttctacacat
     3241 atattcgcaa gatgtggcgt gttacggtga aaacctggcc tatttcccta aagggtttat
     3301 tgagaatatg tttttcgtct cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa
     3361 cgtggccaat atggacaact tcttcgcccc cgttttcacc atgggcaaat attatacgca
     3421 aggcgacaag gtgctgatgc cgctggcgat tcaggttcat catgccgtct gtgatggctt
     3481 ccatgtcggc agaatgctta atgaattaca acagtactgc gatgagtggc agggcggggc
     3541 gtaatcgcgt ggatccggct tactaaaagc cagataacag tatgcgtatt tgcgcgctga
     3601 tttttgcggt ataagaatat atactgatat gtatacccga agtatgtcaa aaagaggtgt
     3661 gctatgaagc agcgtattac agtgacagtt gacagcgaca gctatcagtt gctcaaggca
     3721 tatatgatgt caatatctcc ggtctggtaa gcacaaccat gcagaatgaa gcccgtcgtc
     3781 tgcgtgccga acgctggaaa gcggaaaatc aggaagggat ggctgaggtc gcccggttta
     3841 ttgaaatgaa cggctctttt gctgacgaga acagggactg gtgaaatgca gtttaaggtt
     3901 tacacctata aaagagagag ccgttatcgt ctgtttgtgg atgtacagag tgatattatt
     3961 gacacgcccg ggcgacggat ggtgatcccc ctggccagtg cacgtctgct gtcagataaa
     4021 gtctcccgtg aactttaccc ggtggtgcat atcggggatg aaagctggcg catgatgacc
     4081 accgatatgg ccagtgtgcc ggtctccgtt atcggggaag aagtggctga tctcagccac
     4141 cgcgaaaatg acatcaaaaa cgccattaac ctgatgttct ggggaatata aatgtcaggc
     4201 tcccttatac acagccagtc tgcaggtcga tacagtagaa attacagaaa ctttatcacg
     4261 tttagtaagt atagaggctg aaaatccaga tgaagccgaa cgacttgtaa gagaaaagta
     4321 taagagttgt gaaattgttc ttgatgcaga tgattttcag gactatgaca ctagcgtata
     4381 tgaataggta gatgttttta ttttgtcaca caaaaaagag gctcgcacct ctttttctta
     4441 tttcttttta tgatttaata cggcattgag gacaatagcg agtaggctgg atacgacgat
     4501 tccgtttgag aagaacattt ggaaggctgt cggtcgagct cgaattctac aggtcactaa
     4561 taccatctaa gtagttgatt catagtgact gcatatgttg tgttttacag tattatgtag
     4621 tctgtttttt atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc
     4681 gttcaacttt tctatacaaa gttgatatca ctagtcggcg gccgctctag aggatccaag
     4741 ggcccatttc taaattttag tttaataaaa aaattttaaa ataatacgag ttttgagact
     4801 aactttcaat aaaaaccaaa aaattgcttc aaaaaagagc tcgcgccgat ttacgataaa
     4861 cacagcgcgc tacgacactc cgaaaaccgc gccgcaccat tacattctcc gccccaccgc
     4921 acggtttgcg gaaggcgatg aaattgctgg gcctaaaatt ttaacccttt ttttcccgtt
     4981 tttcaactgt tttcccgatt ttttcgatat ttttttcagg tttttagtta gaaaagtata
     5041 taaaaattgc attatttgta gttttcgctc gtaatttgtg atagaaaacg gccaatttgc
     5101 ctagatttgt gattctacca agtggaattt ttgggaaaat atcaatattc cacgattttt
     5161 attcaaatca taacgtttcg aaattatcag cttaaataaa actaaaataa ataataaaat
     5221 actaaaaacc ctgaatttct cggttctcgg ttcagcttga gttcgttcta tacacgttat
     5281 cgatttttgt catttcgttt aatcatgggt tttgattcat tttaaatatg aaatttgatt
     5341 tattcctcgt ttcgaagttg gtttgtcttg tccggccggt aaacaaatgt tattaaaaaa
     5401 aataataaat ttcgaaattt ttcgtaatgt tccgcaatcg tgtttttccg tttatctcgt
     5461 cttcccttcc ttcaaatata ctatttattt tctcaattca catattttca gccagccctg
     5521 ctcaacaagt ggaataaaaa tgattgaaca agatggattg cacgcaggtt ctccggccgc
     5581 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc
     5641 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc
     5701 cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg
     5761 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt
     5821 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc
     5881 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga
     5941 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga
     6001 tcaggatgat ctggacgaag agcatcaggg gctcgcacca gccgaactgt tcgccaggct
     6061 caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc
     6121 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt
     6181 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg
     6241 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat
     6301 cgccttctat cgccttcttg acgagttctt ctgagtccaa ttactcttca acatccctac
     6361 atgctctttc tccctgtgct cccaccccct atttttgtta ttatcaaaaa acttctctta
     6421 atttctttgt tttttagctt cttttaagcc acctctaaca atgaaattgt gtagattcaa
     6481 aaatagaatt aattcgtaat aaaaagtcga aaaaaattgt gctccctccc cccattaata
     6541 ataattctat cccaaaatct acacaatgtt ctgtgtacac ttcttatgtt ttttacttct
     6601 gataaatttt tttgaaacat catagaaaaa accgcacaca aaatacctta tcatatgtta
     6661 cgtttcagtt tatgaccgca atttttattt cttcgcacgt ctgggcctct catgacgtca
     6721 aatcatgctc atcgtgaaaa agttttggag tatttttgga atttttcaat caagtgaaag
     6781 tttatgaaat taattttcct gcttttgctt tttggggttt cccctattgt ttgtcaagat
     6841 ttcgaggacg gcgtttttct tgctaaaatc acaagtattg atgagcacga tgcaagaaag
     6901 atcggaagaa ggtttgggtt tgaggctcag tggaaggtga gtagaagttg ataatttgaa
     6961 agtggagtag tgtctatggg gtttttgcct taaatgacag aatacattcc caatatacca
     7021 aacataactg tttctcgagg tcgacggtat cgataagctt gatatcgaat tcctgcagcc
     7081 cgggggatcc actagttcta gagcggccgc caccgcggtg gagctccagc ttttgttccc
     7141 tttagtgagg gttaatttcg agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     7201 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     7261 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     7321 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     7381 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     7441 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     7501 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     7561 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     7621 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     7681 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     7741 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     7801 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     7861 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     7921 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     7981 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
     8041 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     8101 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     8161 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     8221 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     8281 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     8341 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     8401 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     8461 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     8521 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     8581 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     8641 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     8701 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     8761 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     8821 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     8881 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     8941 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     9001 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     9061 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     9121 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     9181 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     9241 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     9301 cgcgcacatt tccccgaaaa gtgc
//
