LOCUS       sequence-124354-        1058 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  gRNA targeting human HPRT gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Liao S, Tammaro M, Yan H
  TITLE     Enriching CRISPR-Cas9 targeted cells by co-targeting the HPRT gene.
  JOURNAL   Nucleic Acids Res. 2015 Jun 29. pii: gkv675.
  PUBMED    26130722
REFERENCE   2  (bases 1 to 1058)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Jun 29. pii: gkv675."
COMMENT     Sequence Label: HPRT2b-pGEM
FEATURES             Location/Qualifiers
     source          1..1058
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      complement(105..560)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(242..263)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     454..473
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     686..708
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     700..717
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     701..717
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     724..743
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        724..742
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      773..782
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     861..881
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     1032..1051
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgatgcggt
       61 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggaaattg taagcgttaa
      121 tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc
      181 cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt
      241 tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa
      301 aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg
      361 gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg
      421 acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc
      481 tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa
      541 tgcgccgcta cagggcgcgt ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
      601 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      661 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa
      721 ttgtaatacg actcactata gggcgaattg ggcccgacgt cgcatgctcc cggccgccat
      781 ggccgcggga ttgtacaaaa aagcaggctt taaaggaacc aattcagtcg actggatccg
      841 gtaccaaggt cgggcaggaa gagggcctat ttcccatgat tccttcatat ttgcatatac
      901 gatacaaggc tgttagagag ataattagaa ttaatttgac tgtaaacaca aagatattag
      961 tacaaaatac gtgacgtaga aagtaataat ttcttgggta gtttgcagtt ttaaaattat
     1021 gttttaaaat ggactatcat atgcttaccg taacttga
//
