LOCUS       sequence-124360-        3699 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses humanized Lb3Cpf1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3699)
  AUTHORS   Zetsche B, Gootenberg JS, Abudayyeh OO, Slaymaker IM, Makarova KS, 
            Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev A, Koonin 
            EV, Zhang F
  TITLE     Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas 
            System.
  JOURNAL   Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 
            10.1016/j.cell.2015.09.038.
  PUBMED    26422227
REFERENCE   2  (bases 1 to 3699)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2015 
            Sep 23. pii: S0092-8674(15)01200-3. doi: 
            10.1016/j.cell.2015.09.038."
COMMENT     Sequence Label: pY005 (pcDNA3.1-hLb3Cpf1)
FEATURES             Location/Qualifiers
     source          1..3699
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atggattacg gcaacggcca gtttgagcgg agagcccccc tgaccaagac aatcaccctg
       61 cgcctgaagc ctatcggcga gacacgggag acaatccgcg agcagaagct gctggagcag
      121 gacgccgcct tcagaaagct ggtggagaca gtgaccccta tcgtggacga ttgtatcagg
      181 aagatcgccg ataacgccct gtgccacttt ggcaccgagt atgacttcag ctgtctgggc
      241 aacgccatct ctaagaatga cagcaaggcc atcaagaagg agacagagaa ggtggagaag
      301 ctgctggcca aggtgctgac cgagaatctg ccagatggcc tgcgcaaggt gaacgacatc
      361 aattccgccg cctttatcca ggatacactg acctctttcg tgcaggacga tgccgacaag
      421 cgggtgctga tccaggagct gaagggcaag accgtgctga tgcagcggtt cctgaccaca
      481 cggatcacag ccctgaccgt gtggctgccc gacagagtgt tcgagaactt taatatcttc
      541 atcgagaacg ccgagaagat gagaatcctg ctggactccc ctctgaatga gaagatcatg
      601 aagtttgacc cagatgccga gcagtacgcc tctctggagt tctatggcca gtgcctgtct
      661 cagaaggaca tcgatagcta caacctgatc atctccggca tctatgccga cgatgaggtg
      721 aagaaccctg gcatcaatga gatcgtgaag gagtacaatc agcagatccg gggcgacaag
      781 gatgagtccc cactgcccaa gctgaagaag ctgcacaagc agatcctgat gccagtggag
      841 aaggccttct ttgtgcgcgt gctgtctaac gacagcgatg cccggagcat cctggagaag
      901 atcctgaagg acacagagat gctgccctcc aagatcatcg aggccatgaa ggaggcagat
      961 gcaggcgaca tcgccgtgta cggcagccgg ctgcacgagc tgagccacgt gatctacggc
     1021 gatcacggca agctgtccca gatcatctat gacaaggagt ccaagaggat ctctgagctg
     1081 atggagacac tgtctccaaa ggagcgcaag gagagcaaga agcggctgga gggcctggag
     1141 gagcacatca gaaagtctac atacaccttc gacgagctga acaggtatgc cgagaagaat
     1201 gtgatggcag catacatcgc agcagtggag gagtcttgtg ccgagatcat gagaaaggag
     1261 aaggatctga ggaccctgct gagcaaggag gacgtgaaga tccggggcaa cagacacaat
     1321 acactgatcg tgaagaacta ctttaatgcc tggaccgtgt tccggaacct gatcagaatc
     1381 ctgaggcgca agtccgaggc cgagatcgac tctgacttct acgatgtgct ggacgattcc
     1441 gtggaggtgc tgtctctgac atacaagggc gagaatctgt gccgcagcta tatcaccaag
     1501 aagatcggct ccgacctgaa gcccgagatc gccacatacg gcagcgccct gaggcctaac
     1561 agccgctggt ggtccccagg agagaagttt aatgtgaagt tccacaccat cgtgcggaga
     1621 gatggccggc tgtactattt catcctgccc aagggcgcca agcctgtgga gctggaggac
     1681 atggatggcg acatcgagtg tctgcagatg agaaagatcc ctaacccaac aatctttctg
     1741 cccaagctgg tgttcaagga ccctgaggcc ttctttaggg ataatccaga ggccgacgag
     1801 ttcgtgtttc tgagcggcat gaaggccccc gtgacaatca ccagagagac atacgaggcc
     1861 tacaggtata agctgtatac cgtgggcaag ctgcgcgatg gcgaggtgtc cgaagaggag
     1921 tacaagcggg ccctgctgca ggtgctgacc gcctacaagg agtttctgga gaacagaatg
     1981 atctatgccg acctgaattt cggctttaag gatctggagg agtataagga cagctccgag
     2041 tttatcaagc aggtggagac acacaacacc ttcatgtgct gggccaaggt gtctagctcc
     2101 cagctggacg atctggtgaa gtctggcaac ggcctgctgt tcgagatctg gagcgagcgc
     2161 ctggagtcct actataagta cggcaatgag aaggtgctgc ggggctatga gggcgtgctg
     2221 ctgagcatcc tgaaggatga gaacctggtg tccatgcgga ccctgctgaa cagccggccc
     2281 atgctggtgt accggccaaa ggagtctagc aagcctatgg tggtgcaccg ggatggcagc
     2341 agagtggtgg acaggtttga taaggacggc aagtacatcc cccctgaggt gcacgacgag
     2401 ctgtatcgct tctttaacaa tctgctgatc aaggagaagc tgggcgagaa ggcccggaag
     2461 atcctggaca acaagaaggt gaaggtgaag gtgctggaga gcgagagagt gaagtggtcc
     2521 aagttctacg atgagcagtt tgccgtgacc ttcagcgtga agaagaacgc cgattgtctg
     2581 gacaccacaa aggacctgaa tgccgaagtg atggagcagt atagcgagtc caacagactg
     2641 atcctgatca ggaataccac agatatcctg tactatctgg tgctggacaa gaatggcaag
     2701 gtgctgaagc agagatccct gaacatcatc aatgacggcg ccagggatgt ggactggaag
     2761 gagaggttcc gccaggtgac aaaggataga aacgagggct acaatgagtg ggattattcc
     2821 aggacctcta acgacctgaa ggaggtgtac ctgaattatg ccctgaagga gatcgccgag
     2881 gccgtgatcg agtacaacgc catcctgatc atcgagaaga tgtctaatgc ctttaaggac
     2941 aagtatagct tcctggacga cgtgaccttc aagggcttcg agacaaagct gctggccaag
     3001 ctgagcgatc tgcactttag gggcatcaag gacggcgagc catgttcctt cacaaacccc
     3061 ctgcagctgt gccagaacga ttctaataag atcctgcagg acggcgtgat ctttatggtg
     3121 ccaaattcta tgacacggag cctggacccc gacaccggct tcatctttgc catcaacgac
     3181 cacaatatca ggaccaagaa ggccaagctg aactttctga gcaagttcga tcagctgaag
     3241 gtgtcctctg agggctgcct gatcatgaag tacagcggcg attccctgcc tacacacaac
     3301 accgacaatc gcgtgtggaa ctgctgttgc aatcacccaa tcacaaacta tgaccgggag
     3361 acaaagaagg tggagttcat cgaggagccc gtggaggagc tgtcccgcgt gctggaggag
     3421 aatggcatcg agacagacac cgagctgaac aagctgaatg agcgggagaa cgtgcctggc
     3481 aaggtggtgg atgccatcta ctctctggtg ctgaattatc tgcgcggcac agtgagcgga
     3541 gtggcaggac agagggccgt gtactatagc cctgtgaccg gcaagaagta cgatatctcc
     3601 tttatccagg ccatgaacct gaataggaag tgtgactact ataggatcgg ctccaaggag
     3661 aggggagagt ggaccgattt cgtggcccag ctgatcaac
//
