LOCUS       sequence-124361-        3858 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses humanized BpCpf1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3858)
  AUTHORS   Zetsche B, Gootenberg JS, Abudayyeh OO, Slaymaker IM, Makarova KS, 
            Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev A, Koonin 
            EV, Zhang F
  TITLE     Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas 
            System.
  JOURNAL   Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 
            10.1016/j.cell.2015.09.038.
  PUBMED    26422227
REFERENCE   2  (bases 1 to 3858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2015 
            Sep 23. pii: S0092-8674(15)01200-3. doi: 
            10.1016/j.cell.2015.09.038."
COMMENT     Sequence Label: pY006 (pcDNA3.1-hBpCpf1)
FEATURES             Location/Qualifiers
     source          1..3858
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             3724..3771
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             3778..3858
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
ORIGIN
        1 atgctgctgt atgagaacta caccaagcgg aatcagatca caaagagcct gaggctggag
       61 ctgcgccctc agggcaagac cctgagaaac atcaaggagc tgaatctgct ggagcaggac
      121 aaggccatct acgccctgct ggagcggctg aagccagtga tcgatgaggg catcaaggac
      181 atcgccagag ataccctgaa gaactgcgag ctgtcttttg agaagctgta cgagcacttc
      241 ctgagcggcg acaagaaggc ctatgccaag gagtccgagc ggctgaagaa ggagatcgtg
      301 aaaaccctga tcaagaacct gcccgagggc atcggcaaga tctctgagat caacagcgcc
      361 aagtatctga atggcgtgct gtacgacttc atcgataaga cacacaagga ctctgaggag
      421 aagcagaata tcctgagcga tatcctggag acaaagggct acctggccct gttctccaag
      481 tttctgacat ctcggatcac cacactggag cagtccatgc ctaagagagt gatcgagaac
      541 ttcgagatct atgccgccaa tatcccaaag atgcaggacg ccctggagag gggagccgtg
      601 tcttttgcca tcgagtacga gagcatctgt tccgtggatt actataacca gatcctgtcc
      661 caggaggaca tcgattctta taatcgcctg atcagcggca tcatggacga ggatggcgcc
      721 aaggagaagg gcatcaacca gaccatctcc gagaagaata tcaagatcaa gtctgagcac
      781 ctggaggaga agcccttcag aatcctgaag cagctgcaca agcagatcct ggaggagcgc
      841 gagaaggcct ttacaatcga ccacatcgac agcgatgagg aggtggtgca ggtgaccaag
      901 gaggccttcg agcagacaaa ggagcagtgg gagaacatca agaagatcaa tggcttctac
      961 gccaaggacc ccggcgatat caccctgttt atcgtggtgg gccctaacca gacacacgtg
     1021 ctgtcccagc tgatctacgg cgagcacgac cggatcagac tgctgctgga ggagtatgag
     1081 aagaacaccc tggaggtgct gccccggaga acaaagtctg agaaggccag atacgataag
     1141 ttcgtgaatg ccgtgcctaa gaaggtggcc aaggagagcc acaccttcga cggcctgcag
     1201 aagatgacag gcgacgatcg gctgtttatc ctgtatagag atgagctggc ccggaactac
     1261 atgagaatca aggaggccta tggcaccttt gagcgggaca tcctgaagag caggcgcggc
     1321 atcaagggca atcgggacgt gcaggagtct ctggtgagct tttacgatga gctgacaaag
     1381 ttcaggagcg ccctgcgcat catcaattcc ggcaacgacg agaaggccga tccaatcttc
     1441 tataacacct ttgatggcat cttcgagaag gccaatagga catacaaggc cgagaatctg
     1501 tgccgcaact atgtgaccaa gtctccagcc gacgatgcca ggatcatggc cagctgtctg
     1561 ggcaccccag caaggctgcg cacacactgg tggaatggcg aggagaactt cgccatcaat
     1621 gacgtggcca tgatccggag aggcgatgag tactattact ttgtgctgac cccagacgtg
     1681 aagcccgtgg acctgaaaac caaggacgag acagatgccc agatcttcgt gcagcgcaag
     1741 ggcgccaagt cctttctggg cctgccaaag gccctgttca agtgcatcct ggagccttac
     1801 tttgagtccc cagagcacaa gaatgacaag aactgcgtga tcgaggagta cgtgtctaag
     1861 cccctgacca tcgacaggcg cgcctatgat atctttaaga acggcacctt caagaaaacc
     1921 aatatcggca tcgacggcct gaccgaggag aagttcaagg acgattgccg ctatctgatc
     1981 gacgtgtata aggagttcat cgccgtgtat acaaggtaca gctgttttaa catgtccggc
     2041 ctgaagcgcg ccgacgagta caatgatatc ggcgagttct tttctgacgt ggataccagg
     2101 ctgtgcacaa tggagtggat tcccgtgagc ttcgagcgca tcaacgacat ggtggataag
     2161 aaggagggcc tgctgtttct ggtgaggagc atgttcctgt ataaccggcc cagaaagcct
     2221 tatgagcgca cctttatcca gctgttcagc gactccaaca tggagcacac atctatgctg
     2281 ctgaatagca gggccatgat ccagtacagg gcagcctccc tgccacggag agtgacccac
     2341 aagaagggca gcatcctggt ggccctgcgg gattccaacg gcgagcacat ccccatgcac
     2401 atcagagagg ccatctacaa gatgaagaac aattttgaca tcagctccga ggatttcatc
     2461 atggccaagg cctatctggc cgagcacgac gtggccatca agaaggccaa cgaggatatc
     2521 atcaggaata ggcgctacac cgaggacaag ttctttctgt ctctgagcta taccaagaac
     2581 gccgatatca gcgcccgcac actggactac atcaatgata aggtggagga ggacacacag
     2641 gattccagga tggccgtgat cgtgacccgc aacctgaagg acctgacata cgtggccgtg
     2701 gtggatgaga agaacaatgt gctggaggag aagagcctga acgagatcga cggcgtgaat
     2761 tatcgggagc tgctgaagga gagaaccaag atcaagtacc acgacaagac acggctgtgg
     2821 cagtatgacg tgagcagcaa gggcctgaag gaggcctacg tggagctggc cgtgacccag
     2881 atctccaagc tggccacaaa gtataacgcc gtggtggtgg tggagtccat gtcctctacc
     2941 ttcaaggaca agttctcttt tctggatgag cagatcttca aggcctttga ggcccggctg
     3001 tgcgccagaa tgtccgacct gtcttttaat acaatcaagg agggcgaggc cggctccatc
     3061 tctaacccca tccaggtgtc caacaataac ggcaattctt atcaggacgg cgtgatctac
     3121 ttcctgaata acgcctatac ccggaccctg tgccctgata ccggctttgt ggacgtgttc
     3181 gataagaccc ggctgatcac aatgcagtct aagagacagt tctttgccaa gatgaaggac
     3241 atcagaatcg acgatggcga gatgctgttc acctttaacc tggaggagta ccctacaaag
     3301 aggctgctgg accgcaagga gtggaccgtg aagatcgccg gcgatggctc ctatttcgac
     3361 aaggataagg gcgagtacgt gtacgtgaac gacatcgtga gagagcagat catcccagcc
     3421 ctgctggagg acaaggccgt gttcgatggc aatatggccg agaagtttct ggataagacc
     3481 gccatcagcg gcaagtccgt ggagctgatc tacaagtggt tcgccaacgc cctgtatggc
     3541 atcatcacaa agaaggacgg cgagaagatc taccggagcc ccatcaccgg cacagagatc
     3601 gacgtgagca agaacaccac atacaacttc ggcaagaagt tcatgttcaa gcaggagtat
     3661 agaggcgacg gcgattttct ggacgccttc ctgaattaca tgcaggccca ggatatcgcc
     3721 gtgaaaaggc cggcggccac gaaaaaggcc ggccaggcaa aaaagaaaaa gggatcctac
     3781 ccatacgatg ttccagatta cgcttatccc tacgacgtgc ctgattatgc atacccatat
     3841 gatgtccccg actatgcc
//
