LOCUS       sequence-124362-        4569 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses humanized PeCpf1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4569)
  AUTHORS   Zetsche B, Gootenberg JS, Abudayyeh OO, Slaymaker IM, Makarova KS, 
            Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev A, Koonin 
            EV, Zhang F
  TITLE     Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas 
            System.
  JOURNAL   Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 
            10.1016/j.cell.2015.09.038.
  PUBMED    26422227
REFERENCE   2  (bases 1 to 4569)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2015 
            Sep 23. pii: S0092-8674(15)01200-3. doi: 
            10.1016/j.cell.2015.09.038."
COMMENT     Sequence Label: pY007 (pcDNA3.1-hPeCpf1)
FEATURES             Location/Qualifiers
     source          1..4569
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4432..4479
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4486..4566
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
ORIGIN
        1 atgtccaact tctttaagaa tttcaccaac ctgtatgagc tgtccaagac actgaggttt
       61 gagctgaagc ccgtgggcga caccctgaca aacatgaagg accacctgga gtacgatgag
      121 aagctgcaga ccttcctgaa ggatcagaat atcgacgatg cctatcaggc cctgaagcct
      181 cagttcgacg agatccacga ggagtttatc acagattctc tggagagcaa gaaggccaag
      241 gagatcgact tctccgagta cctggatctg tttcaggaga agaaggagct gaacgactct
      301 gagaagaagc tgcgcaacaa gatcggcgag acattcaaca aggccggcga gaagtggaag
      361 aaggagaagt accctcagta tgagtggaag aagggctcca agatcgccaa tggcgccgac
      421 atcctgtctt gccaggatat gctgcagttt atcaagtata agaacccaga ggatgagaag
      481 atcaagaatt acatcgacga tacactgaag ggcttcttta cctatttcgg cggctttaat
      541 cagaacaggg ccaactacta tgagacaaag aaggaggcct ccaccgcagt ggcaacaagg
      601 atcgtgcacg agaacctgcc aaagttctgt gacaatgtga tccagtttaa gcacatcatc
      661 aagcggaaga aggatggcac cgtggagaaa accgagagaa agaccgagta cctgaacgcc
      721 taccagtatc tgaagaacaa taacaagatc acacagatca aggacgccga gacagagaag
      781 atgatcgagt ctacacccat cgccgagaag atcttcgacg tgtactactt cagcagctgc
      841 ctgagccaga agcagatcga ggagtacaac cggatcatcg gccactataa tctgctgatc
      901 aacctgtata accaggccaa gagatctgag ggcaagcacc tgagcgccaa cgagaagaag
      961 tataaggacc tgcctaagtt caagaccctg tataagcaga tcggctgcgg caagaagaag
     1021 gacctgtttt acacaatcaa gtgtgatacc gaggaggagg ccaataagtc ccggaacgag
     1081 ggcaaggagt cccactctgt ggaggagatc atcaacaagg cccaggaggc catcaataag
     1141 tacttcaagt ctaataacga ctgtgagaat atcaacaccg tgcccgactt catcaactat
     1201 atcctgacaa aggagaatta cgagggcgtg tattggagca aggccgccat gaacaccatc
     1261 tccgacaagt acttcgccaa ttatcacgac ctgcaggata gactgaagga ggccaaggtg
     1321 tttcagaagg ccgataagaa gtccgaggac gatatcaaga tcccagaggc catcgagctg
     1381 tctggcctgt tcggcgtgct ggacagcctg gccgattggc agaccacact gtttaagtct
     1441 agcatcctga gcaacgagga caagctgaag atcatcacag attcccagac cccctctgag
     1501 gccctgctga agatgatctt caatgacatc gagaagaaca tggagtcctt tctgaaggag
     1561 acaaacgata tcatcaccct gaagaagtat aagggcaata aggagggcac cgagaagatc
     1621 aagcagtggt tcgactatac actggccatc aaccggatgc tgaagtactt tctggtgaag
     1681 gagaataaga tcaagggcaa ctccctggat accaatatct ctgaggccct gaaaaccctg
     1741 atctacagcg acgatgccga gtggttcaag tggtacgacg ccctgagaaa ctatctgacc
     1801 cagaagcctc aggatgaggc caaggagaat aagctgaagc tgaatttcga caacccatct
     1861 ctggccggcg gctgggatgt gaacaaggag tgcagcaatt tttgcgtgat cctgaaggac
     1921 aagaacgaga agaagtacct ggccatcatg aagaagggcg agaataccct gttccagaag
     1981 gagtggacag agggccgggg caagaacctg acaaagaagt ctaatccact gttcgagatc
     2041 aataactgcg agatcctgag caagatggag tatgactttt gggccgacgt gagcaagatg
     2101 atccccaagt gtagcaccca gctgaaggcc gtggtgaacc acttcaagca gtccgacaat
     2161 gagttcatct ttcctatcgg ctacaaggtg acaagcggcg agaagtttag ggaggagtgc
     2221 aagatctcca agcaggactt cgagctgaat aacaaggtgt ttaataagaa cgagctgagc
     2281 gtgaccgcca tgcgctacga tctgtcctct acacaggaga agcagtatat caaggccttc
     2341 cagaaggagt actgggagct gctgtttaag caggagaagc gggacaccaa gctgacaaat
     2401 aacgagatct tcaacgagtg gatcaatttt tgcaacaaga agtatagcga gctgctgtcc
     2461 tgggagagaa agtacaagga tgccctgacc aattggatca acttctgtaa gtactttctg
     2521 agcaagtatc ccaagaccac actgttcaac tactctttta aggagagcga gaattataac
     2581 tccctggacg agttctaccg ggacgtggat atctgttctt acaagctgaa tatcaacacc
     2641 acaatcaata agagcatcct ggatagactg gtggaggagg gcaagctgta cctgtttgag
     2701 atcaagaatc aggacagcaa cgatggcaag tccatcggcc acaagaataa cctgcacacc
     2761 atctactgga acgccatctt cgagaatttt gacaacaggc ctaagctgaa tggcgaggcc
     2821 gagatcttct atcgcaaggc catctccaag gataagctgg gcatcgtgaa gggcaagaaa
     2881 accaagaacg gcaccgagat catcaagaat tacagattca gcaaggagaa gtttatcctg
     2941 cacgtgccaa tcaccctgaa cttctgctcc aataacgagt atgtgaatga catcgtgaac
     3001 acaaagttct acaatttttc caacctgcac tttctgggca tcgatagggg cgagaagcac
     3061 ctggcctact attctctggt gaataagaac ggcgagatcg tggaccaggg cacactgaac
     3121 ctgcctttca ccgacaagga tggcaatcag cgcagcatca agaaggagaa gtacttttat
     3181 aacaagcagg aggacaagtg ggaggccaag gaggtggatt gttggaatta taacgacctg
     3241 ctggatgcca tggcctctaa ccgggacatg gccagaaaga attggcagag gatcggcacc
     3301 atcaaggagg ccaagaacgg ctacgtgagc ctggtcatca ggaagatcgc cgatctggcc
     3361 gtgaataacg agcgccccgc cttcatcgtg ctggaggacc tgaatacagg ctttaagcgg
     3421 tccagacaga agatcgataa gagcgtgtac cagaagttcg agctggccct ggccaagaag
     3481 ctgaactttc tggtggacaa gaatgccaag cgcgatgaga tcggctcccc tacaaaggcc
     3541 ctgcagctga ccccccctgt gaataactac ggcgacattg agaacaagaa gcaggccggc
     3601 atcatgctgt atacccgggc caattatacc tctcagacag atccagccac aggctggaga
     3661 aagaccatct atctgaaggc cggccccgag gagacaacat acaagaagga cggcaagatc
     3721 aagaacaaga gcgtgaagga ccagatcatc gagacattca ccgatatcgg ctttgacggc
     3781 aaggattact atttcgagta cgacaagggc gagtttgtgg atgagaaaac cggcgagatc
     3841 aagcccaaga agtggcggct gtactccggc gagaatggca agtccctgga caggttccgc
     3901 ggagagaggg agaaggataa gtatgagtgg aagatcgaca agatcgatat cgtgaagatc
     3961 ctggacgatc tgttcgtgaa ttttgacaag aacatcagcc tgctgaagca gctgaaggag
     4021 ggcgtggagc tgacccggaa taacgagcac ggcacaggcg agtccctgag attcgccatc
     4081 aacctgatcc agcagatccg gaataccggc aataacgaga gagacaacga tttcatcctg
     4141 tccccagtga gggacgagaa tggcaagcac tttgactctc gcgagtactg ggataaggag
     4201 acaaagggcg agaagatcag catgcccagc tccggcgatg ccaatggcgc cttcaacatc
     4261 gcccggaagg gcatcatcat gaacgcccac atcctggcca atagcgactc caaggatctg
     4321 tccctgttcg tgtctgacga ggagtgggat ctgcacctga ataacaagac cgagtggaag
     4381 aagcagctga acatcttttc tagcaggaag gccatggcca agcgcaagaa gaaaaggccg
     4441 gcggccacga aaaaggccgg ccaggcaaaa aagaaaaagg gatcctaccc atacgatgtt
     4501 ccagattacg cttatcccta cgacgtgcct gattatgcat acccatatga tgtccccgac
     4561 tatgcctaa
//
